Salmonella phage UPF_BP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4FNB3|A0A2H4FNB3_9CAUD Uncharacterized protein OS=Salmonella phage UPF_BP1 OX=1912492 GN=SPUPF_00075 PE=4 SV=1
MM1 pKa = 7.49TIITRR6 pKa = 11.84EE7 pKa = 3.84RR8 pKa = 11.84LLTIQQWRR16 pKa = 11.84EE17 pKa = 3.67TYY19 pKa = 10.46GPGSNVVLPAEE30 pKa = 4.27EE31 pKa = 4.5AEE33 pKa = 4.18EE34 pKa = 4.01LARR37 pKa = 11.84IALVSLEE44 pKa = 4.36AEE46 pKa = 4.37PVSEE50 pKa = 4.28TYY52 pKa = 10.7NLPEE56 pKa = 5.38LIEE59 pKa = 4.21GMEE62 pKa = 3.92VSIDD66 pKa = 3.9VSTCDD71 pKa = 3.23ADD73 pKa = 3.4AGNRR77 pKa = 11.84YY78 pKa = 9.3FGTVTEE84 pKa = 4.21VSEE87 pKa = 5.39LYY89 pKa = 9.2TARR92 pKa = 11.84NGYY95 pKa = 9.16ILLVQDD101 pKa = 4.58AKK103 pKa = 11.37PNFDD107 pKa = 3.71VNGNTPVQSPIDD119 pKa = 3.24HH120 pKa = 7.27GYY122 pKa = 10.35RR123 pKa = 11.84PEE125 pKa = 4.51CEE127 pKa = 4.14CSGCKK132 pKa = 8.57ATARR136 pKa = 11.84ICAEE140 pKa = 4.16LNNEE144 pKa = 3.97EE145 pKa = 4.63AEE147 pKa = 4.27IFADD151 pKa = 5.03GYY153 pKa = 9.1NAAMQSFGNSEE164 pKa = 4.02QLNSPVTPDD173 pKa = 2.71GWISCSEE180 pKa = 3.93RR181 pKa = 11.84MPDD184 pKa = 3.36NDD186 pKa = 3.34EE187 pKa = 4.51SKK189 pKa = 10.6PIAIFTGKK197 pKa = 10.33CLGQGMFVATYY208 pKa = 10.9DD209 pKa = 3.46DD210 pKa = 5.34DD211 pKa = 5.81AFFDD215 pKa = 3.73YY216 pKa = 10.54WEE218 pKa = 4.39GMEE221 pKa = 4.89IIGVTHH227 pKa = 7.05WMPLPEE233 pKa = 4.85PPQEE237 pKa = 4.03EE238 pKa = 4.62VKK240 pKa = 10.96

Molecular weight: 26.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4FWM7|A0A2H4FWM7_9CAUD Uncharacterized protein OS=Salmonella phage UPF_BP1 OX=1912492 GN=SPUPF_0003 PE=4 SV=1
MM1 pKa = 7.98DD2 pKa = 4.9GASKK6 pKa = 10.35RR7 pKa = 11.84VSRR10 pKa = 11.84AISLAGTRR18 pKa = 11.84QKK20 pKa = 10.6EE21 pKa = 4.34VEE23 pKa = 4.11VTAVKK28 pKa = 10.37KK29 pKa = 10.53NRR31 pKa = 11.84IYY33 pKa = 11.07YY34 pKa = 10.02RR35 pKa = 11.84DD36 pKa = 3.78VNPLGNKK43 pKa = 7.92IHH45 pKa = 6.76AVQRR49 pKa = 11.84MKK51 pKa = 10.97LSSKK55 pKa = 10.39PLII58 pKa = 4.18

Molecular weight: 6.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

12302

32

725

173.3

19.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.535 ± 0.524

1.187 ± 0.148

5.983 ± 0.257

6.698 ± 0.394

3.325 ± 0.235

7.007 ± 0.382

1.512 ± 0.18

5.926 ± 0.241

6.479 ± 0.308

7.69 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.869 ± 0.169

4.918 ± 0.208

3.853 ± 0.214

4.552 ± 0.363

5.812 ± 0.298

6.357 ± 0.249

5.292 ± 0.261

6.015 ± 0.291

1.618 ± 0.154

3.373 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski