Nocardioides immobilis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6094 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A417XZB1|A0A417XZB1_9ACTN Spirocyclase AveC family OS=Nocardioides immobilis OX=2049295 GN=D0Z08_18275 PE=4 SV=1
MM1 pKa = 7.48TFRR4 pKa = 11.84ALALCFLTAGALAAPIGSPEE24 pKa = 4.14GATASGPPTCGGMAATIVGTSGNDD48 pKa = 3.32VLTGTAGDD56 pKa = 3.96DD57 pKa = 3.91VIVGLKK63 pKa = 10.18GDD65 pKa = 4.03DD66 pKa = 4.16VIDD69 pKa = 4.36GGLGADD75 pKa = 4.65RR76 pKa = 11.84ICGRR80 pKa = 11.84GGDD83 pKa = 4.67DD84 pKa = 4.67SITGNGGTDD93 pKa = 3.23HH94 pKa = 7.21LFGGAGVDD102 pKa = 3.84YY103 pKa = 10.28MDD105 pKa = 4.93GGVGGCCDD113 pKa = 3.48ASANTGDD120 pKa = 4.39DD121 pKa = 3.74VLSGGIGDD129 pKa = 5.06DD130 pKa = 3.76NLHH133 pKa = 6.72ASDD136 pKa = 5.9FSTTGNTIHH145 pKa = 6.57GRR147 pKa = 11.84QGADD151 pKa = 3.8DD152 pKa = 3.93IYY154 pKa = 10.63LYY156 pKa = 10.95SGGAAYY162 pKa = 10.01GGKK165 pKa = 10.38GRR167 pKa = 11.84DD168 pKa = 3.47TLSQYY173 pKa = 11.41AGDD176 pKa = 3.81ATLEE180 pKa = 4.23GGIGADD186 pKa = 3.33TLGNANNPAIDD197 pKa = 3.59HH198 pKa = 5.56VIIRR202 pKa = 11.84GGDD205 pKa = 3.38GSDD208 pKa = 3.11TLTSDD213 pKa = 3.48DD214 pKa = 4.0TTGTTAMNGNRR225 pKa = 11.84GRR227 pKa = 11.84DD228 pKa = 3.31VCTGGTSTVHH238 pKa = 6.61CEE240 pKa = 3.85SS241 pKa = 3.77

Molecular weight: 23.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A417Y1Z7|A0A417Y1Z7_9ACTN Aspartate 1-decarboxylase OS=Nocardioides immobilis OX=2049295 GN=panD PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6094

0

6094

1939228

26

2341

318.2

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.859 ± 0.046

0.802 ± 0.009

6.668 ± 0.026

5.952 ± 0.03

2.867 ± 0.018

9.144 ± 0.026

2.214 ± 0.013

3.778 ± 0.02

1.971 ± 0.021

10.047 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.859 ± 0.011

1.896 ± 0.018

5.614 ± 0.025

2.785 ± 0.014

7.638 ± 0.035

5.212 ± 0.023

5.972 ± 0.022

9.157 ± 0.028

1.526 ± 0.012

2.04 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski