Acidovorax sp. (strain JS42)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Acidovorax; unclassified Acidovorax

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4045 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1W5X6|A1W5X6_ACISJ Uncharacterized protein OS=Acidovorax sp. (strain JS42) OX=232721 GN=Ajs_1440 PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 6.64PILAALPLSLLKK14 pKa = 10.72LVEE17 pKa = 4.51GSLSNDD23 pKa = 2.79EE24 pKa = 4.32VSSDD28 pKa = 3.84EE29 pKa = 4.09EE30 pKa = 3.88MLGYY34 pKa = 10.51FIDD37 pKa = 4.0NGLTEE42 pKa = 4.09EE43 pKa = 4.3QARR46 pKa = 11.84QALTYY51 pKa = 10.19RR52 pKa = 11.84DD53 pKa = 3.41QYY55 pKa = 11.38LNNIYY60 pKa = 10.62LDD62 pKa = 3.71GFTPITAVDD71 pKa = 3.7EE72 pKa = 4.47ALHH75 pKa = 6.07FNPHH79 pKa = 4.8TQQFEE84 pKa = 3.9PDD86 pKa = 3.07

Molecular weight: 9.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1WDD1|A1WDD1_ACISJ Homoserine dehydrogenase OS=Acidovorax sp. (strain JS42) OX=232721 GN=Ajs_4155 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4045

0

4045

1298069

24

2026

320.9

34.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.255 ± 0.059

0.978 ± 0.013

5.128 ± 0.03

5.273 ± 0.03

3.289 ± 0.023

8.198 ± 0.041

2.43 ± 0.021

4.048 ± 0.029

3.157 ± 0.037

10.784 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.019

2.431 ± 0.022

5.451 ± 0.03

4.54 ± 0.029

7.159 ± 0.041

4.991 ± 0.027

5.122 ± 0.024

7.581 ± 0.034

1.529 ± 0.019

2.189 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski