Lactobacillus mucosae LM1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Limosilactobacillus; Limosilactobacillus mucosae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1970 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D4CNW9|A0A0D4CNW9_LACMC Uncharacterized protein OS=Lactobacillus mucosae LM1 OX=1130798 GN=LBLM1_11105 PE=4 SV=1
MM1 pKa = 7.88LDD3 pKa = 3.46GLGDD7 pKa = 3.95SVTWYY12 pKa = 10.76CDD14 pKa = 3.06NCDD17 pKa = 4.25DD18 pKa = 4.55IMPVGHH24 pKa = 6.49EE25 pKa = 4.42AYY27 pKa = 10.49RR28 pKa = 11.84MPDD31 pKa = 3.18GTILCSEE38 pKa = 4.52VCVDD42 pKa = 5.5DD43 pKa = 3.75YY44 pKa = 12.04AFMHH48 pKa = 6.9NDD50 pKa = 3.36DD51 pKa = 3.91TTKK54 pKa = 10.98ADD56 pKa = 3.52SDD58 pKa = 3.77ILAEE62 pKa = 4.1TWDD65 pKa = 3.65KK66 pKa = 11.78VEE68 pKa = 4.09TFVIPRR74 pKa = 11.84EE75 pKa = 4.09DD76 pKa = 3.68EE77 pKa = 3.79II78 pKa = 6.23

Molecular weight: 8.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D4CHX0|A0A0D4CHX0_LACMC Oxidoreductase OS=Lactobacillus mucosae LM1 OX=1130798 GN=LBLM1_00585 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.02KK9 pKa = 7.4RR10 pKa = 11.84HH11 pKa = 5.36RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 5.84GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1970

0

1970

621732

38

3774

315.6

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.634 ± 0.062

0.592 ± 0.015

6.122 ± 0.057

5.618 ± 0.058

4.005 ± 0.041

6.586 ± 0.049

2.351 ± 0.025

6.501 ± 0.053

6.286 ± 0.053

9.65 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.828 ± 0.033

4.65 ± 0.046

3.732 ± 0.041

4.992 ± 0.057

4.413 ± 0.045

5.547 ± 0.051

5.801 ± 0.072

6.908 ± 0.051

1.154 ± 0.021

3.628 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski