Nitrosospira sp. Nl5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosospira; unclassified Nitrosospira

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2892 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5DY89|A0A1G5DY89_9PROT Murein hydrolase A OS=Nitrosospira sp. Nl5 OX=200120 GN=SAMN05216420_103140 PE=4 SV=1
MM1 pKa = 7.86TDD3 pKa = 4.23TIPQASRR10 pKa = 11.84DD11 pKa = 3.79YY12 pKa = 11.33DD13 pKa = 3.3NTLIIEE19 pKa = 4.57RR20 pKa = 11.84PDD22 pKa = 2.8GFYY25 pKa = 9.83WHH27 pKa = 7.8DD28 pKa = 3.65EE29 pKa = 4.3NEE31 pKa = 4.3SEE33 pKa = 4.96RR34 pKa = 11.84VFGPFPTLLDD44 pKa = 5.11AIQDD48 pKa = 3.6MEE50 pKa = 4.53YY51 pKa = 10.65NAEE54 pKa = 3.9SDD56 pKa = 4.15YY57 pKa = 11.59EE58 pKa = 4.19PGEE61 pKa = 4.07TLEE64 pKa = 4.12QAEE67 pKa = 4.44EE68 pKa = 4.26EE69 pKa = 4.2IGISSWIDD77 pKa = 3.23HH78 pKa = 5.84EE79 pKa = 4.45TGEE82 pKa = 4.5PGEE85 pKa = 4.22EE86 pKa = 4.38SFPHH90 pKa = 7.12DD91 pKa = 3.43NN92 pKa = 3.85

Molecular weight: 10.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5HII4|A0A1G5HII4_9PROT ABC-type uncharacterized transport system permease component OS=Nitrosospira sp. Nl5 OX=200120 GN=SAMN05216420_11119 PE=4 SV=1
MM1 pKa = 7.52CKK3 pKa = 10.39VSVEE7 pKa = 4.15RR8 pKa = 11.84ACRR11 pKa = 11.84LGNFSRR17 pKa = 11.84GGWYY21 pKa = 9.79RR22 pKa = 11.84KK23 pKa = 9.46SRR25 pKa = 11.84ARR27 pKa = 11.84DD28 pKa = 3.34QSGLRR33 pKa = 11.84MRR35 pKa = 11.84IRR37 pKa = 11.84DD38 pKa = 3.23IAMSRR43 pKa = 11.84PRR45 pKa = 11.84FGYY48 pKa = 10.09EE49 pKa = 3.74RR50 pKa = 11.84IHH52 pKa = 6.26VLLRR56 pKa = 11.84RR57 pKa = 11.84EE58 pKa = 4.09GWHH61 pKa = 6.41VNLEE65 pKa = 4.34STGCIAWKK73 pKa = 10.14VFKK76 pKa = 10.16CACGYY81 pKa = 10.87AEE83 pKa = 4.34RR84 pKa = 11.84SVV86 pKa = 3.13

Molecular weight: 10.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2892

0

2892

867502

26

2888

300.0

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.175 ± 0.055

0.974 ± 0.015

5.256 ± 0.035

6.053 ± 0.037

3.851 ± 0.029

7.797 ± 0.045

2.386 ± 0.024

5.803 ± 0.032

4.294 ± 0.037

10.371 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.559 ± 0.025

3.448 ± 0.029

4.838 ± 0.033

3.74 ± 0.029

6.411 ± 0.037

5.941 ± 0.033

5.176 ± 0.035

6.901 ± 0.04

1.304 ± 0.02

2.721 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski