Proteome-pI 2.0 - Proteome Isoelectric Point Database

Beihai narna-like virus 14

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.99

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1L3KHV4|A0A1L3KHV4_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 14 OX=1922441 PE=4 SV=1

MM1 pKa = 6.44TTAIPTDD8 pKa = 3.62QLSKK12 pKa = 10.94AFKK15 pKa = 10.12ISLATFFSKK24 pKa = 10.96EE25 pKa = 3.9NGQEE29 pKa = 3.73MPEE32 pKa = 4.49GYY34 pKa = 10.19KK35 pKa = 10.28SAFRR39 pKa = 11.84LFPNRR44 pKa = 11.84FYY46 pKa = 11.05PLLRR50 pKa = 11.84KK51 pKa = 9.61RR52 pKa = 11.84WTKK55 pKa = 9.18KK56 pKa = 8.6ARR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84TRR62 pKa = 11.84EE63 pKa = 3.31TFMLLKK69 pKa = 10.27AKK71 pKa = 10.08RR72 pKa = 11.84LSAPVPRR79 pKa = 11.84EE80 pKa = 4.14MIRR83 pKa = 11.84KK84 pKa = 8.87AYY86 pKa = 9.03EE87 pKa = 3.85SHH89 pKa = 6.66GSLLSTVGKK98 pKa = 8.54TPEE101 pKa = 4.42DD102 pKa = 3.34VLLTLRR108 pKa = 11.84LVARR112 pKa = 11.84EE113 pKa = 3.27WAEE116 pKa = 4.0RR117 pKa = 11.84VRR119 pKa = 11.84HH120 pKa = 6.24HH121 pKa = 6.89YY122 pKa = 10.9DD123 pKa = 2.86EE124 pKa = 5.4TIPLGPSKK132 pKa = 10.8AYY134 pKa = 9.93FGCPRR139 pKa = 11.84SSGGCFSSLKK149 pKa = 10.3NLYY152 pKa = 10.19SRR154 pKa = 11.84LNTRR158 pKa = 11.84GNPLMVGTRR167 pKa = 11.84VDD169 pKa = 3.43PPLIYY174 pKa = 10.71LFGRR178 pKa = 11.84PGVGKK183 pKa = 10.46SFICNSIVRR192 pKa = 11.84SLSEE196 pKa = 4.28CFGEE200 pKa = 5.59GPASVYY206 pKa = 8.08WRR208 pKa = 11.84NYY210 pKa = 4.73TTEE213 pKa = 3.7HH214 pKa = 6.59HH215 pKa = 7.16DD216 pKa = 3.86GYY218 pKa = 9.46TGQLIYY224 pKa = 10.77GIDD227 pKa = 3.52DD228 pKa = 4.1AFQNRR233 pKa = 11.84DD234 pKa = 3.43SVSAPDD240 pKa = 3.55SVRR243 pKa = 11.84DD244 pKa = 3.67EE245 pKa = 4.64IIQLRR250 pKa = 11.84SNNPFTMKK258 pKa = 9.81MARR261 pKa = 11.84LEE263 pKa = 4.26DD264 pKa = 3.1KK265 pKa = 10.85GRR267 pKa = 11.84NFNSEE272 pKa = 4.18FLLLSSNLSPEE283 pKa = 4.04QVVACTDD290 pKa = 4.38HH291 pKa = 6.12IHH293 pKa = 6.98CSAALGRR300 pKa = 11.84RR301 pKa = 11.84MKK303 pKa = 10.44TSFEE307 pKa = 3.99IKK309 pKa = 10.33DD310 pKa = 3.89FNHH313 pKa = 5.92KK314 pKa = 8.6TRR316 pKa = 11.84LYY318 pKa = 9.85RR319 pKa = 11.84ITVNEE324 pKa = 3.96LVNLEE329 pKa = 3.97GRR331 pKa = 11.84SPRR334 pKa = 11.84LEE336 pKa = 3.46QRR338 pKa = 11.84RR339 pKa = 11.84YY340 pKa = 10.68LEE342 pKa = 4.31LDD344 pKa = 3.13LRR346 pKa = 11.84SLVALIVRR354 pKa = 11.84WAVDD358 pKa = 3.18EE359 pKa = 4.47HH360 pKa = 7.44RR361 pKa = 11.84DD362 pKa = 4.12AITHH366 pKa = 6.71ALASTGRR373 pKa = 11.84EE374 pKa = 3.96RR375 pKa = 11.84GWRR378 pKa = 11.84IPMINYY384 pKa = 9.58GPGRR388 pKa = 11.84PCLGYY393 pKa = 10.08EE394 pKa = 4.54FPSEE398 pKa = 4.21LPEE401 pKa = 4.28KK402 pKa = 10.42NQVEE406 pKa = 4.74AYY408 pKa = 10.26AISEE412 pKa = 4.14PLKK415 pKa = 10.61VRR417 pKa = 11.84LITKK421 pKa = 7.49EE422 pKa = 3.88QPLSYY427 pKa = 10.89AMKK430 pKa = 9.29PLQRR434 pKa = 11.84AMTRR438 pKa = 11.84ALRR441 pKa = 11.84DD442 pKa = 3.8FNCFFPGWKK451 pKa = 8.36GTLEE455 pKa = 4.35DD456 pKa = 4.75FVNLNLAGQKK466 pKa = 10.18GHH468 pKa = 6.96ILSGDD473 pKa = 3.44YY474 pKa = 10.71SAATDD479 pKa = 3.8NLNSDD484 pKa = 3.72VMNTVLEE491 pKa = 4.22EE492 pKa = 4.16VKK494 pKa = 10.56KK495 pKa = 10.49VFPGHH500 pKa = 6.54HH501 pKa = 6.93LLHH504 pKa = 6.86KK505 pKa = 10.63YY506 pKa = 10.59VDD508 pKa = 4.58FFGGPHH514 pKa = 5.87EE515 pKa = 4.19VLYY518 pKa = 9.52PKK520 pKa = 8.24WTKK523 pKa = 10.71LPAIQQRR530 pKa = 11.84RR531 pKa = 11.84GQLMGSLLSFPVLCIANAATLCHH554 pKa = 6.34VRR556 pKa = 11.84QQDD559 pKa = 3.55LHH561 pKa = 5.81EE562 pKa = 5.2LKK564 pKa = 10.92ACINGDD570 pKa = 3.96DD571 pKa = 4.03ILFVDD576 pKa = 4.23TNRR579 pKa = 11.84CIRR582 pKa = 11.84RR583 pKa = 11.84WKK585 pKa = 10.34KK586 pKa = 9.84VATSLGLVPSIGKK599 pKa = 8.74NFQDD603 pKa = 3.7MSFGTINSEE612 pKa = 3.95LLFRR616 pKa = 11.84RR617 pKa = 11.84KK618 pKa = 9.74KK619 pKa = 10.79GNTFRR624 pKa = 11.84SADD627 pKa = 3.32TGKK630 pKa = 9.92PGLLVRR636 pKa = 11.84KK637 pKa = 9.5NMPLAHH643 pKa = 6.9LALKK647 pKa = 10.22EE648 pKa = 4.46GYY650 pKa = 10.08SKK652 pKa = 11.25GQVVLWGKK660 pKa = 9.8PSLEE664 pKa = 3.98RR665 pKa = 11.84HH666 pKa = 6.28PGSIDD671 pKa = 3.15VPLNFGGLGVEE682 pKa = 4.82FDD684 pKa = 3.66PSRR687 pKa = 11.84RR688 pKa = 11.84PTRR691 pKa = 11.84KK692 pKa = 9.69DD693 pKa = 2.77RR694 pKa = 11.84SLYY697 pKa = 10.44LFHH700 pKa = 7.9ADD702 pKa = 3.03RR703 pKa = 11.84LLRR706 pKa = 11.84HH707 pKa = 5.92IRR709 pKa = 11.84QVSRR713 pKa = 11.84VEE715 pKa = 3.95MTPGRR720 pKa = 11.84SLVCLPRR727 pKa = 11.84ALAKK731 pKa = 10.69GPLRR735 pKa = 11.84SLCEE739 pKa = 3.9DD740 pKa = 3.15ARR742 pKa = 11.84QQVADD747 pKa = 4.46FRR749 pKa = 11.84LVAQSVMDD757 pKa = 3.84TLLTEE762 pKa = 4.42QEE764 pKa = 4.96AEE766 pKa = 4.25FPWRR770 pKa = 11.84EE771 pKa = 3.35FHH773 pKa = 7.4LFRR776 pKa = 11.84RR777 pKa = 11.84SAWEE781 pKa = 3.48KK782 pKa = 8.92CKK784 pKa = 10.31RR785 pKa = 11.84WRR787 pKa = 11.84DD788 pKa = 3.83FLASGSLEE796 pKa = 3.99TCQPLTALTPHH807 pKa = 6.4WTTVSTEE814 pKa = 4.2DD815 pKa = 4.82LPFIDD820 pKa = 5.3NYY822 pKa = 7.82TSRR825 pKa = 11.84WTTSQLFSRR834 pKa = 11.84EE835 pKa = 3.95TRR837 pKa = 11.84RR838 pKa = 11.84TPCPVV843 pKa = 2.69

Molecular weight: 96.32 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1L3KHV4|A0A1L3KHV4_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 14 OX=1922441 PE=4 SV=1

MM1 pKa = 6.44TTAIPTDD8 pKa = 3.62QLSKK12 pKa = 10.94AFKK15 pKa = 10.12ISLATFFSKK24 pKa = 10.96EE25 pKa = 3.9NGQEE29 pKa = 3.73MPEE32 pKa = 4.49GYY34 pKa = 10.19KK35 pKa = 10.28SAFRR39 pKa = 11.84LFPNRR44 pKa = 11.84FYY46 pKa = 11.05PLLRR50 pKa = 11.84KK51 pKa = 9.61RR52 pKa = 11.84WTKK55 pKa = 9.18KK56 pKa = 8.6ARR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84TRR62 pKa = 11.84EE63 pKa = 3.31TFMLLKK69 pKa = 10.27AKK71 pKa = 10.08RR72 pKa = 11.84LSAPVPRR79 pKa = 11.84EE80 pKa = 4.14MIRR83 pKa = 11.84KK84 pKa = 8.87AYY86 pKa = 9.03EE87 pKa = 3.85SHH89 pKa = 6.66GSLLSTVGKK98 pKa = 8.54TPEE101 pKa = 4.42DD102 pKa = 3.34VLLTLRR108 pKa = 11.84LVARR112 pKa = 11.84EE113 pKa = 3.27WAEE116 pKa = 4.0RR117 pKa = 11.84VRR119 pKa = 11.84HH120 pKa = 6.24HH121 pKa = 6.89YY122 pKa = 10.9DD123 pKa = 2.86EE124 pKa = 5.4TIPLGPSKK132 pKa = 10.8AYY134 pKa = 9.93FGCPRR139 pKa = 11.84SSGGCFSSLKK149 pKa = 10.3NLYY152 pKa = 10.19SRR154 pKa = 11.84LNTRR158 pKa = 11.84GNPLMVGTRR167 pKa = 11.84VDD169 pKa = 3.43PPLIYY174 pKa = 10.71LFGRR178 pKa = 11.84PGVGKK183 pKa = 10.46SFICNSIVRR192 pKa = 11.84SLSEE196 pKa = 4.28CFGEE200 pKa = 5.59GPASVYY206 pKa = 8.08WRR208 pKa = 11.84NYY210 pKa = 4.73TTEE213 pKa = 3.7HH214 pKa = 6.59HH215 pKa = 7.16DD216 pKa = 3.86GYY218 pKa = 9.46TGQLIYY224 pKa = 10.77GIDD227 pKa = 3.52DD228 pKa = 4.1AFQNRR233 pKa = 11.84DD234 pKa = 3.43SVSAPDD240 pKa = 3.55SVRR243 pKa = 11.84DD244 pKa = 3.67EE245 pKa = 4.64IIQLRR250 pKa = 11.84SNNPFTMKK258 pKa = 9.81MARR261 pKa = 11.84LEE263 pKa = 4.26DD264 pKa = 3.1KK265 pKa = 10.85GRR267 pKa = 11.84NFNSEE272 pKa = 4.18FLLLSSNLSPEE283 pKa = 4.04QVVACTDD290 pKa = 4.38HH291 pKa = 6.12IHH293 pKa = 6.98CSAALGRR300 pKa = 11.84RR301 pKa = 11.84MKK303 pKa = 10.44TSFEE307 pKa = 3.99IKK309 pKa = 10.33DD310 pKa = 3.89FNHH313 pKa = 5.92KK314 pKa = 8.6TRR316 pKa = 11.84LYY318 pKa = 9.85RR319 pKa = 11.84ITVNEE324 pKa = 3.96LVNLEE329 pKa = 3.97GRR331 pKa = 11.84SPRR334 pKa = 11.84LEE336 pKa = 3.46QRR338 pKa = 11.84RR339 pKa = 11.84YY340 pKa = 10.68LEE342 pKa = 4.31LDD344 pKa = 3.13LRR346 pKa = 11.84SLVALIVRR354 pKa = 11.84WAVDD358 pKa = 3.18EE359 pKa = 4.47HH360 pKa = 7.44RR361 pKa = 11.84DD362 pKa = 4.12AITHH366 pKa = 6.71ALASTGRR373 pKa = 11.84EE374 pKa = 3.96RR375 pKa = 11.84GWRR378 pKa = 11.84IPMINYY384 pKa = 9.58GPGRR388 pKa = 11.84PCLGYY393 pKa = 10.08EE394 pKa = 4.54FPSEE398 pKa = 4.21LPEE401 pKa = 4.28KK402 pKa = 10.42NQVEE406 pKa = 4.74AYY408 pKa = 10.26AISEE412 pKa = 4.14PLKK415 pKa = 10.61VRR417 pKa = 11.84LITKK421 pKa = 7.49EE422 pKa = 3.88QPLSYY427 pKa = 10.89AMKK430 pKa = 9.29PLQRR434 pKa = 11.84AMTRR438 pKa = 11.84ALRR441 pKa = 11.84DD442 pKa = 3.8FNCFFPGWKK451 pKa = 8.36GTLEE455 pKa = 4.35DD456 pKa = 4.75FVNLNLAGQKK466 pKa = 10.18GHH468 pKa = 6.96ILSGDD473 pKa = 3.44YY474 pKa = 10.71SAATDD479 pKa = 3.8NLNSDD484 pKa = 3.72VMNTVLEE491 pKa = 4.22EE492 pKa = 4.16VKK494 pKa = 10.56KK495 pKa = 10.49VFPGHH500 pKa = 6.54HH501 pKa = 6.93LLHH504 pKa = 6.86KK505 pKa = 10.63YY506 pKa = 10.59VDD508 pKa = 4.58FFGGPHH514 pKa = 5.87EE515 pKa = 4.19VLYY518 pKa = 9.52PKK520 pKa = 8.24WTKK523 pKa = 10.71LPAIQQRR530 pKa = 11.84RR531 pKa = 11.84GQLMGSLLSFPVLCIANAATLCHH554 pKa = 6.34VRR556 pKa = 11.84QQDD559 pKa = 3.55LHH561 pKa = 5.81EE562 pKa = 5.2LKK564 pKa = 10.92ACINGDD570 pKa = 3.96DD571 pKa = 4.03ILFVDD576 pKa = 4.23TNRR579 pKa = 11.84CIRR582 pKa = 11.84RR583 pKa = 11.84WKK585 pKa = 10.34KK586 pKa = 9.84VATSLGLVPSIGKK599 pKa = 8.74NFQDD603 pKa = 3.7MSFGTINSEE612 pKa = 3.95LLFRR616 pKa = 11.84RR617 pKa = 11.84KK618 pKa = 9.74KK619 pKa = 10.79GNTFRR624 pKa = 11.84SADD627 pKa = 3.32TGKK630 pKa = 9.92PGLLVRR636 pKa = 11.84KK637 pKa = 9.5NMPLAHH643 pKa = 6.9LALKK647 pKa = 10.22EE648 pKa = 4.46GYY650 pKa = 10.08SKK652 pKa = 11.25GQVVLWGKK660 pKa = 9.8PSLEE664 pKa = 3.98RR665 pKa = 11.84HH666 pKa = 6.28PGSIDD671 pKa = 3.15VPLNFGGLGVEE682 pKa = 4.82FDD684 pKa = 3.66PSRR687 pKa = 11.84RR688 pKa = 11.84PTRR691 pKa = 11.84KK692 pKa = 9.69DD693 pKa = 2.77RR694 pKa = 11.84SLYY697 pKa = 10.44LFHH700 pKa = 7.9ADD702 pKa = 3.03RR703 pKa = 11.84LLRR706 pKa = 11.84HH707 pKa = 5.92IRR709 pKa = 11.84QVSRR713 pKa = 11.84VEE715 pKa = 3.95MTPGRR720 pKa = 11.84SLVCLPRR727 pKa = 11.84ALAKK731 pKa = 10.69GPLRR735 pKa = 11.84SLCEE739 pKa = 3.9DD740 pKa = 3.15ARR742 pKa = 11.84QQVADD747 pKa = 4.46FRR749 pKa = 11.84LVAQSVMDD757 pKa = 3.84TLLTEE762 pKa = 4.42QEE764 pKa = 4.96AEE766 pKa = 4.25FPWRR770 pKa = 11.84EE771 pKa = 3.35FHH773 pKa = 7.4LFRR776 pKa = 11.84RR777 pKa = 11.84SAWEE781 pKa = 3.48KK782 pKa = 8.92CKK784 pKa = 10.31RR785 pKa = 11.84WRR787 pKa = 11.84DD788 pKa = 3.83FLASGSLEE796 pKa = 3.99TCQPLTALTPHH807 pKa = 6.4WTTVSTEE814 pKa = 4.2DD815 pKa = 4.82LPFIDD820 pKa = 5.3NYY822 pKa = 7.82TSRR825 pKa = 11.84WTTSQLFSRR834 pKa = 11.84EE835 pKa = 3.95TRR837 pKa = 11.84RR838 pKa = 11.84TPCPVV843 pKa = 2.69

Molecular weight: 96.32 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	843	843	843	843.0	96.32

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
5.931 ± 0.0	2.017 ± 0.0	4.508 ± 0.0	5.813 ± 0.0	4.982 ± 0.0	6.287 ± 0.0	2.728 ± 0.0	3.677 ± 0.0	5.338 ± 0.0	11.507 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.017 ± 0.0	3.915 ± 0.0	5.931 ± 0.0	2.966 ± 0.0	9.371 ± 0.0	7.236 ± 0.0	5.931 ± 0.0	5.457 ± 0.0	1.661 ± 0.0	2.728 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski