Streptococcus phage IPP44

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SCX3|A0A1S5SCX3_9CAUD Tail tape measure family protein OS=Streptococcus phage IPP44 OX=1916183 GN=IPP44_00039 PE=4 SV=1
MM1 pKa = 7.34MEE3 pKa = 4.11EE4 pKa = 4.65LKK6 pKa = 10.91QKK8 pKa = 10.59VNEE11 pKa = 4.25VYY13 pKa = 10.57NWTVEE18 pKa = 4.14DD19 pKa = 4.66GKK21 pKa = 10.07PKK23 pKa = 10.17PPKK26 pKa = 9.56QDD28 pKa = 2.86LPQAVKK34 pKa = 10.63EE35 pKa = 4.03RR36 pKa = 11.84VDD38 pKa = 3.95YY39 pKa = 9.78FWEE42 pKa = 4.03MAEE45 pKa = 5.33DD46 pKa = 3.88GMTFMGAMEE55 pKa = 5.67CIFADD60 pKa = 4.73EE61 pKa = 5.02KK62 pKa = 10.3PTDD65 pKa = 3.84YY66 pKa = 11.56DD67 pKa = 4.51LGATKK72 pKa = 10.55DD73 pKa = 3.52WLPKK77 pKa = 10.51SKK79 pKa = 10.64EE80 pKa = 3.52FDD82 pKa = 3.25DD83 pKa = 3.91WVGYY87 pKa = 8.92SPSMAQVVIAVYY99 pKa = 10.24LIYY102 pKa = 10.77GGSEE106 pKa = 3.96DD107 pKa = 3.8EE108 pKa = 4.32

Molecular weight: 12.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SCY4|A0A1S5SCY4_9CAUD Lytic amidase OS=Streptococcus phage IPP44 OX=1916183 GN=IPP44_00045 PE=4 SV=1
MM1 pKa = 7.54NEE3 pKa = 3.8IVWFYY8 pKa = 10.24FVVIINLIIGFATYY22 pKa = 10.08YY23 pKa = 10.25ASKK26 pKa = 10.35RR27 pKa = 11.84DD28 pKa = 3.27RR29 pKa = 11.84KK30 pKa = 10.49KK31 pKa = 10.81RR32 pKa = 11.84INEE35 pKa = 3.81YY36 pKa = 10.94KK37 pKa = 10.55KK38 pKa = 10.48IQDD41 pKa = 4.18DD42 pKa = 3.61EE43 pKa = 4.84LEE45 pKa = 4.38RR46 pKa = 11.84IRR48 pKa = 11.84NKK50 pKa = 10.42FNLL53 pKa = 3.75

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

9471

40

1506

210.5

24.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.42 ± 0.608

0.581 ± 0.131

5.828 ± 0.31

8.595 ± 0.39

3.938 ± 0.248

5.786 ± 0.368

1.615 ± 0.182

6.926 ± 0.311

8.383 ± 0.377

8.964 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.439 ± 0.255

5.744 ± 0.302

2.481 ± 0.238

4.002 ± 0.282

4.498 ± 0.307

6.525 ± 0.405

6.219 ± 0.348

5.828 ± 0.272

1.489 ± 0.192

3.738 ± 0.394
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski