Halorhodospira halophila (strain DSM 244 / SL1) (Ectothiorhodospira halophila (strain DSM 244 / SL1))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Halorhodospira; Halorhodospira halophila

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2406 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A1WWW0|HFLD_HALHL High frequency lysogenization protein HflD homolog OS=Halorhodospira halophila (strain DSM 244 / SL1) OX=349124 GN=hflD PE=3 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84LTRR5 pKa = 11.84RR6 pKa = 11.84AVYY9 pKa = 8.35FAPCLLALGGIATSAHH25 pKa = 5.59GAAFQLQEE33 pKa = 3.95QSASLLASAFAGRR46 pKa = 11.84SADD49 pKa = 3.63AGDD52 pKa = 3.57ISHH55 pKa = 6.68MFFNPATLGVHH66 pKa = 7.16AGRR69 pKa = 11.84GPEE72 pKa = 3.91AEE74 pKa = 4.65LVLSYY79 pKa = 10.65IDD81 pKa = 3.39PTFDD85 pKa = 4.31FEE87 pKa = 5.99ADD89 pKa = 3.56SDD91 pKa = 4.44PGWSAVGGDD100 pKa = 3.83TGQLSASGGEE110 pKa = 4.19SALAPVFYY118 pKa = 10.51AGFDD122 pKa = 3.66LAPDD126 pKa = 3.98LRR128 pKa = 11.84AGLGINVPYY137 pKa = 10.51GLEE140 pKa = 3.75TDD142 pKa = 4.2YY143 pKa = 11.72PEE145 pKa = 4.79DD146 pKa = 3.08WVGRR150 pKa = 11.84YY151 pKa = 9.23DD152 pKa = 4.75AVNTDD157 pKa = 4.09LVTVDD162 pKa = 4.75INPQLGWRR170 pKa = 11.84LDD172 pKa = 3.57EE173 pKa = 4.21QLALGLGVSAQYY185 pKa = 11.74ADD187 pKa = 3.68ATLSTMAPEE196 pKa = 4.68TDD198 pKa = 4.24FSTTPATPTPTPTPANDD215 pKa = 3.52GKK217 pKa = 9.87LTVDD221 pKa = 3.67GDD223 pKa = 3.58SWSYY227 pKa = 11.34SFNLGAHH234 pKa = 5.5YY235 pKa = 10.57AVTEE239 pKa = 4.03DD240 pKa = 3.57TQLGVAYY247 pKa = 10.25RR248 pKa = 11.84HH249 pKa = 5.9GLGHH253 pKa = 6.0TLSGEE258 pKa = 4.12AEE260 pKa = 4.16LVDD263 pKa = 4.43DD264 pKa = 5.07GGVTVDD270 pKa = 3.68EE271 pKa = 4.8TGGEE275 pKa = 4.01ADD277 pKa = 4.2LDD279 pKa = 3.86IPASLNLGVSHH290 pKa = 6.72QLDD293 pKa = 4.08PQWTLLADD301 pKa = 3.86ATWTQWSDD309 pKa = 3.55FEE311 pKa = 4.2EE312 pKa = 4.28LAIEE316 pKa = 4.22FDD318 pKa = 4.12DD319 pKa = 5.72GIVGIPQGQNSYY331 pKa = 11.38AVTDD335 pKa = 3.63EE336 pKa = 4.17QAYY339 pKa = 10.1EE340 pKa = 4.05EE341 pKa = 4.7YY342 pKa = 10.61DD343 pKa = 3.39WDD345 pKa = 4.14DD346 pKa = 2.94TWFLSLGTRR355 pKa = 11.84YY356 pKa = 9.88DD357 pKa = 4.6ASEE360 pKa = 3.96QWDD363 pKa = 3.9LRR365 pKa = 11.84AGLAYY370 pKa = 10.41DD371 pKa = 3.46QTPTGDD377 pKa = 3.31SNRR380 pKa = 11.84TPRR383 pKa = 11.84IPDD386 pKa = 3.32EE387 pKa = 4.42DD388 pKa = 4.12RR389 pKa = 11.84TWLAVGGTWSPAQADD404 pKa = 3.65QLRR407 pKa = 11.84VDD409 pKa = 4.49FGYY412 pKa = 9.54TYY414 pKa = 10.67IWLDD418 pKa = 3.53DD419 pKa = 3.82ADD421 pKa = 4.13IEE423 pKa = 4.43VAEE426 pKa = 4.83DD427 pKa = 3.61FGGVTGEE434 pKa = 4.28YY435 pKa = 9.68EE436 pKa = 4.41SNVQVFTTSANWRR449 pKa = 11.84FF450 pKa = 3.38

Molecular weight: 48.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1WWG0|A1WWG0_HALHL Biopolymer transport protein ExbD/TolR OS=Halorhodospira halophila (strain DSM 244 / SL1) OX=349124 GN=Hhal_1247 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.19QPSNIKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 7.88RR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84LVLKK32 pKa = 10.32RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 9.0RR41 pKa = 11.84LTPTAA46 pKa = 4.02

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2406

0

2406

815824

36

1896

339.1

37.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.968 ± 0.072

0.905 ± 0.017

6.066 ± 0.039

7.489 ± 0.051

3.057 ± 0.029

8.891 ± 0.046

2.45 ± 0.023

4.094 ± 0.035

1.76 ± 0.038

10.72 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.983 ± 0.022

2.01 ± 0.024

5.381 ± 0.041

3.859 ± 0.029

8.709 ± 0.056

4.521 ± 0.03

4.81 ± 0.03

7.518 ± 0.041

1.434 ± 0.022

2.378 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski