bacterium A52C2

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4028 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9MV20|A0A1H9MV20_9BACT Uncharacterized protein OS=bacterium A52C2 OX=1855383 GN=SAMN05216548_11462 PE=4 SV=1
MM1 pKa = 7.35FVRR4 pKa = 11.84ITEE7 pKa = 4.09EE8 pKa = 3.67EE9 pKa = 3.81AALIAFVPALASVTEE24 pKa = 4.51RR25 pKa = 11.84YY26 pKa = 9.03KK27 pKa = 10.64ATASEE32 pKa = 3.92AAAAEE37 pKa = 4.22SEE39 pKa = 4.07IAIARR44 pKa = 11.84EE45 pKa = 3.94RR46 pKa = 11.84YY47 pKa = 9.95ADD49 pKa = 4.66DD50 pKa = 5.13DD51 pKa = 5.3LEE53 pKa = 6.16IDD55 pKa = 4.69DD56 pKa = 5.98DD57 pKa = 5.16PIVSNAEE64 pKa = 3.61NGVWVSAWVWVPSEE78 pKa = 4.27TFVCEE83 pKa = 4.16CCNGEE88 pKa = 3.82WTLDD92 pKa = 3.39EE93 pKa = 4.69RR94 pKa = 11.84ADD96 pKa = 3.7SGLCKK101 pKa = 10.26EE102 pKa = 4.83CEE104 pKa = 3.8RR105 pKa = 11.84TQAAEE110 pKa = 4.12EE111 pKa = 4.11EE112 pKa = 4.36NEE114 pKa = 4.27DD115 pKa = 4.02LCRR118 pKa = 11.84TCGAQYY124 pKa = 11.03EE125 pKa = 4.38DD126 pKa = 4.68GGDD129 pKa = 3.89GFDD132 pKa = 5.26GEE134 pKa = 5.22CPSCADD140 pKa = 3.23KK141 pKa = 10.87TDD143 pKa = 3.7QKK145 pKa = 11.15LHH147 pKa = 6.41PEE149 pKa = 3.89NYY151 pKa = 9.38EE152 pKa = 3.95DD153 pKa = 4.9QEE155 pKa = 4.2

Molecular weight: 17.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9NBI1|A0A1H9NBI1_9BACT Methyltransferase domain-containing protein OS=bacterium A52C2 OX=1855383 GN=SAMN05216548_11565 PE=4 SV=1
MM1 pKa = 7.51FSPAFRR7 pKa = 11.84KK8 pKa = 10.32AGTATKK14 pKa = 10.01GLSAAVLDD22 pKa = 4.35LLLPPVCPACRR33 pKa = 11.84APVGEE38 pKa = 4.11PHH40 pKa = 7.22ALCARR45 pKa = 11.84CWSEE49 pKa = 3.27IRR51 pKa = 11.84FIEE54 pKa = 4.64KK55 pKa = 9.78PYY57 pKa = 10.15CPVYY61 pKa = 9.55GTPFAYY67 pKa = 10.24RR68 pKa = 11.84LGSSVLSAEE77 pKa = 4.48ALADD81 pKa = 3.86PPPFDD86 pKa = 4.14RR87 pKa = 11.84SRR89 pKa = 11.84AATAFGDD96 pKa = 3.44ITQDD100 pKa = 3.31LVHH103 pKa = 6.12QLKK106 pKa = 9.76YY107 pKa = 10.29HH108 pKa = 6.74DD109 pKa = 4.28RR110 pKa = 11.84PEE112 pKa = 4.24VASFMARR119 pKa = 11.84AMARR123 pKa = 11.84AGAEE127 pKa = 3.99LLEE130 pKa = 4.77GEE132 pKa = 4.72TLLIPIPLHH141 pKa = 6.33RR142 pKa = 11.84WRR144 pKa = 11.84LWRR147 pKa = 11.84RR148 pKa = 11.84RR149 pKa = 11.84FNQAAVLARR158 pKa = 11.84KK159 pKa = 8.7IAEE162 pKa = 4.1MTSTGFDD169 pKa = 2.81ARR171 pKa = 11.84TLVRR175 pKa = 11.84IRR177 pKa = 11.84ATRR180 pKa = 11.84QQVGLSGSQRR190 pKa = 11.84RR191 pKa = 11.84LNVQSAFLVPEE202 pKa = 3.86AMKK205 pKa = 10.41IRR207 pKa = 11.84VQGRR211 pKa = 11.84HH212 pKa = 4.86VVLVDD217 pKa = 3.65DD218 pKa = 5.3VYY220 pKa = 11.58TSGATVKK227 pKa = 10.55AATRR231 pKa = 11.84ALLRR235 pKa = 11.84AGAARR240 pKa = 11.84VDD242 pKa = 3.65VLTFARR248 pKa = 11.84VLRR251 pKa = 4.37

Molecular weight: 27.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4028

0

4028

1253240

29

2487

311.1

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.343 ± 0.059

0.79 ± 0.011

5.711 ± 0.031

5.872 ± 0.043

3.723 ± 0.024

8.821 ± 0.043

2.026 ± 0.016

5.032 ± 0.031

3.023 ± 0.037

10.084 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.361 ± 0.018

2.508 ± 0.021

5.338 ± 0.035

3.097 ± 0.024

7.41 ± 0.044

5.647 ± 0.029

5.257 ± 0.024

7.541 ± 0.031

1.298 ± 0.015

2.117 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski