Sewage-associated circular DNA virus-29

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UGN4|A0A0B4UGN4_9VIRU Coat protein OS=Sewage-associated circular DNA virus-29 OX=1592096 PE=4 SV=1
MM1 pKa = 7.46FNYY4 pKa = 9.74QRR6 pKa = 11.84FQQDD10 pKa = 2.58EE11 pKa = 4.61RR12 pKa = 11.84EE13 pKa = 4.31LKK15 pKa = 10.35FCYY18 pKa = 9.85TNIGVSSLSWVGQRR32 pKa = 11.84FPVNRR37 pKa = 11.84IPRR40 pKa = 11.84GYY42 pKa = 10.95DD43 pKa = 2.89NVNQRR48 pKa = 11.84TGRR51 pKa = 11.84VITQHH56 pKa = 4.96FCKK59 pKa = 10.44LRR61 pKa = 11.84GFLQLQKK68 pKa = 9.91TEE70 pKa = 5.08DD71 pKa = 3.91IPWTGPNGDD80 pKa = 4.71FSPSQITRR88 pKa = 11.84LMIIEE93 pKa = 4.75DD94 pKa = 4.19LQPTGAGFTLTDD106 pKa = 4.73LFQEE110 pKa = 4.77TPSQYY115 pKa = 11.51ALPSDD120 pKa = 4.3FKK122 pKa = 11.0FSNINRR128 pKa = 11.84FRR130 pKa = 11.84IWFDD134 pKa = 2.7KK135 pKa = 10.78SYY137 pKa = 10.71PFDD140 pKa = 3.61AFAYY144 pKa = 6.45KK145 pKa = 10.06TGVIEE150 pKa = 4.37GFVNRR155 pKa = 11.84TIYY158 pKa = 10.22WIDD161 pKa = 3.42EE162 pKa = 4.76DD163 pKa = 4.25IDD165 pKa = 3.7LHH167 pKa = 6.61GLQTVYY173 pKa = 10.6GASPGVDD180 pKa = 2.74ILTSSLTFVAVSTANTPPVGGTQNGTSGSFRR211 pKa = 11.84CTIGFYY217 pKa = 10.99DD218 pKa = 4.37DD219 pKa = 3.55

Molecular weight: 24.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UGZ5|A0A0B4UGZ5_9VIRU ATP-dependent helicase Rep OS=Sewage-associated circular DNA virus-29 OX=1592096 PE=3 SV=1
MM1 pKa = 7.26SRR3 pKa = 11.84QVGQKK8 pKa = 8.19WKK10 pKa = 10.7APKK13 pKa = 9.81QLQAPRR19 pKa = 11.84NAFTNNNRR27 pKa = 11.84NPKK30 pKa = 10.17ANGRR34 pKa = 11.84SAPLATRR41 pKa = 11.84GFGDD45 pKa = 3.36MGILKK50 pKa = 9.9NRR52 pKa = 11.84EE53 pKa = 3.66KK54 pKa = 11.0KK55 pKa = 10.68FFDD58 pKa = 3.79RR59 pKa = 11.84STLGAPTGVSTTPVAYY75 pKa = 10.49LLHH78 pKa = 6.67NPAQGADD85 pKa = 3.32YY86 pKa = 10.17NQRR89 pKa = 11.84IGRR92 pKa = 11.84KK93 pKa = 6.49TVVKK97 pKa = 10.13SIYY100 pKa = 9.14IRR102 pKa = 11.84GRR104 pKa = 11.84MFVEE108 pKa = 4.34PVGLTPLPADD118 pKa = 3.25QWCPGQQARR127 pKa = 11.84LIVFIDD133 pKa = 3.66YY134 pKa = 10.33QPNGATPALTDD145 pKa = 4.56LLNSADD151 pKa = 4.18PASQLNADD159 pKa = 3.16NRR161 pKa = 11.84DD162 pKa = 3.24RR163 pKa = 11.84FKK165 pKa = 11.22VVKK168 pKa = 10.57DD169 pKa = 3.36KK170 pKa = 11.51VFTFGPVCVSNSDD183 pKa = 3.75LVSSEE188 pKa = 4.7RR189 pKa = 11.84SYY191 pKa = 11.92QDD193 pKa = 2.88FKK195 pKa = 11.42FFKK198 pKa = 10.29KK199 pKa = 10.72LNLEE203 pKa = 4.7CINNSTSTNAIADD216 pKa = 3.88IQSGALFAYY225 pKa = 9.63FIGTNVAGSTDD236 pKa = 4.18LVSVWTSRR244 pKa = 11.84TRR246 pKa = 11.84YY247 pKa = 10.1EE248 pKa = 4.66DD249 pKa = 3.16MM250 pKa = 5.39

Molecular weight: 27.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

996

219

527

332.0

37.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.827 ± 1.085

1.305 ± 0.033

7.129 ± 0.427

5.622 ± 1.714

4.217 ± 1.705

6.024 ± 1.004

1.205 ± 0.323

5.924 ± 0.589

7.329 ± 1.735

6.426 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.807 ± 0.327

5.823 ± 0.543

6.225 ± 0.284

4.518 ± 0.739

5.321 ± 0.611

6.827 ± 0.129

6.526 ± 0.893

5.522 ± 0.425

1.807 ± 0.202

3.614 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski