Pararcticibacter amylolyticus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pararcticibacter

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4966 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U2PLG4|A0A2U2PLG4_9SPHI Uncharacterized protein OS=Pararcticibacter amylolyticus OX=2173175 GN=DDR33_03660 PE=4 SV=1
MM1 pKa = 7.49EE2 pKa = 6.1DD3 pKa = 3.23EE4 pKa = 4.55YY5 pKa = 11.68LFEE8 pKa = 5.94KK9 pKa = 9.09EE10 pKa = 3.98QKK12 pKa = 10.01IPEE15 pKa = 4.23DD16 pKa = 3.48PFYY19 pKa = 10.96IEE21 pKa = 5.71ADD23 pKa = 3.6VFDD26 pKa = 4.02TGDD29 pKa = 3.44YY30 pKa = 10.94SRR32 pKa = 11.84LLVVPCGTKK41 pKa = 10.61YY42 pKa = 10.64ILVLNDD48 pKa = 3.1EE49 pKa = 4.69HH50 pKa = 7.86LCTLEE55 pKa = 4.2LTCDD59 pKa = 3.76EE60 pKa = 4.47PVCWEE65 pKa = 4.03QEE67 pKa = 3.97EE68 pKa = 5.08GNLDD72 pKa = 3.69DD73 pKa = 6.08EE74 pKa = 4.55IVEE77 pKa = 4.31RR78 pKa = 11.84LGKK81 pKa = 10.22AINGYY86 pKa = 7.87SVV88 pKa = 2.75

Molecular weight: 10.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U2PGZ2|A0A2U2PGZ2_9SPHI Glycoside hydrolase OS=Pararcticibacter amylolyticus OX=2173175 GN=DDR33_10975 PE=4 SV=1
MM1 pKa = 7.81IPGQYY6 pKa = 8.74RR7 pKa = 11.84DD8 pKa = 3.88HH9 pKa = 6.92SGKK12 pKa = 10.28SATFLRR18 pKa = 11.84LGCEE22 pKa = 3.74PAATQTRR29 pKa = 11.84GRR31 pKa = 11.84HH32 pKa = 4.44EE33 pKa = 3.83QDD35 pKa = 2.78TRR37 pKa = 11.84KK38 pKa = 7.92TRR40 pKa = 11.84GKK42 pKa = 9.49HH43 pKa = 4.14AASRR47 pKa = 11.84LLPRR51 pKa = 11.84NCPGTILVKK60 pKa = 10.79NADD63 pKa = 3.41QQ64 pKa = 4.11

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4966

0

4966

1864360

21

8378

375.4

42.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.307 ± 0.039

0.75 ± 0.011

5.358 ± 0.024

5.824 ± 0.041

4.844 ± 0.024

7.266 ± 0.036

1.705 ± 0.018

6.691 ± 0.034

6.488 ± 0.034

9.272 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.147 ± 0.016

5.432 ± 0.035

3.963 ± 0.018

3.497 ± 0.019

4.678 ± 0.027

7.087 ± 0.037

5.639 ± 0.042

6.449 ± 0.031

1.277 ± 0.014

4.327 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski