SR1 bacterium human oral taxon HOT-345

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Absconditabacteria; unclassified Candidatus Absconditabacteria

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1055 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328EGG9|A0A328EGG9_9BACT Uncharacterized protein OS=SR1 bacterium human oral taxon HOT-345 OX=1540874 GN=BSK20_01815 PE=4 SV=1
MM1 pKa = 6.79LTKK4 pKa = 10.31RR5 pKa = 11.84QIYY8 pKa = 9.74LVIFAVLSVLLAVGTLFYY26 pKa = 10.95LRR28 pKa = 11.84EE29 pKa = 4.03LDD31 pKa = 4.53RR32 pKa = 11.84RR33 pKa = 11.84LQEE36 pKa = 3.93PLQEE40 pKa = 3.89EE41 pKa = 5.16FIYY44 pKa = 10.9EE45 pKa = 4.22EE46 pKa = 4.53TPVVPVSDD54 pKa = 4.78LSSSSGADD62 pKa = 3.33YY63 pKa = 10.78LQEE66 pKa = 5.16DD67 pKa = 4.84FDD69 pKa = 5.37ALLSGEE75 pKa = 4.63TVADD79 pKa = 3.71MYY81 pKa = 11.03EE82 pKa = 4.01GEE84 pKa = 4.25RR85 pKa = 11.84DD86 pKa = 3.52YY87 pKa = 11.83GFTGSQNN94 pKa = 3.08

Molecular weight: 10.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328EHU0|A0A328EHU0_9BACT Uncharacterized protein OS=SR1 bacterium human oral taxon HOT-345 OX=1540874 GN=BSK20_00930 PE=4 SV=1
MM1 pKa = 7.5ASKK4 pKa = 10.44LRR6 pKa = 11.84RR7 pKa = 11.84IRR9 pKa = 11.84KK10 pKa = 6.35YY11 pKa = 9.76QWTKK15 pKa = 10.61RR16 pKa = 11.84IRR18 pKa = 11.84THH20 pKa = 6.32GFRR23 pKa = 11.84EE24 pKa = 4.39RR25 pKa = 11.84MNSWTGRR32 pKa = 11.84RR33 pKa = 11.84VLARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84AKK41 pKa = 10.22GRR43 pKa = 11.84HH44 pKa = 4.27QLTVQRR50 pKa = 11.84QKK52 pKa = 11.54

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1055

0

1055

325055

49

4354

308.1

34.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.158 ± 0.082

0.957 ± 0.029

5.001 ± 0.059

7.254 ± 0.093

5.254 ± 0.068

6.891 ± 0.079

1.645 ± 0.03

7.28 ± 0.087

8.491 ± 0.081

10.258 ± 0.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.04

4.765 ± 0.087

3.581 ± 0.056

4.367 ± 0.056

3.939 ± 0.059

6.464 ± 0.07

5.354 ± 0.076

5.597 ± 0.071

0.78 ± 0.022

3.775 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski