Porcine stool-associated circular virus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; Porprismacovirus porci3

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N0ACH8|N0ACH8_9VIRU Cap protein OS=Porcine stool-associated circular virus 3 OX=1328032 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.63VKK4 pKa = 9.23FTQFFDD10 pKa = 3.19ISSSSEE16 pKa = 3.51GFTTFKK22 pKa = 10.83VVAGGDD28 pKa = 3.83YY29 pKa = 10.71VKK31 pKa = 10.32QRR33 pKa = 11.84CMPQFLCYY41 pKa = 9.81KK42 pKa = 8.85YY43 pKa = 10.6YY44 pKa = 10.99KK45 pKa = 10.07LGPVSVKK52 pKa = 10.28LVPASTLPVDD62 pKa = 3.88PTGLSYY68 pKa = 10.46ATGEE72 pKa = 4.23NTVDD76 pKa = 3.47PRR78 pKa = 11.84DD79 pKa = 3.66MFNPGLVRR87 pKa = 11.84ITNGEE92 pKa = 3.93FVDD95 pKa = 4.76TVSSMDD101 pKa = 3.23TYY103 pKa = 11.41YY104 pKa = 10.65ATLLDD109 pKa = 3.84PRR111 pKa = 11.84WFKK114 pKa = 10.86FQLQQGFSRR123 pKa = 11.84YY124 pKa = 9.42ARR126 pKa = 11.84PLVYY130 pKa = 10.62NIGEE134 pKa = 4.04IHH136 pKa = 6.49QDD138 pKa = 3.37PVGLTGQSIPDD149 pKa = 3.46DD150 pKa = 3.88SGINSGFNYY159 pKa = 10.32YY160 pKa = 10.29DD161 pKa = 3.4VSGQKK166 pKa = 10.45LKK168 pKa = 10.88LDD170 pKa = 3.89SYY172 pKa = 10.13STNNLGNAISYY183 pKa = 10.64SPIQTGRR190 pKa = 11.84IKK192 pKa = 10.6LGWMPTDD199 pKa = 3.22TPTFTNGTQAGGLLSFVPEE218 pKa = 3.74VDD220 pKa = 3.47VITFVLPKK228 pKa = 10.27AFKK231 pKa = 8.69TKK233 pKa = 9.12FYY235 pKa = 10.48YY236 pKa = 10.06RR237 pKa = 11.84VFVEE241 pKa = 3.98STVYY245 pKa = 10.58FKK247 pKa = 11.29DD248 pKa = 3.39PVAINPIVAANGKK261 pKa = 8.88VAFPMDD267 pKa = 3.88RR268 pKa = 11.84FVGLQAVNGGSLSQTWTNAQLGYY291 pKa = 10.35HH292 pKa = 5.38YY293 pKa = 10.59KK294 pKa = 10.64EE295 pKa = 4.33EE296 pKa = 4.27NEE298 pKa = 4.11PP299 pKa = 3.65

Molecular weight: 33.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N0ACH8|N0ACH8_9VIRU Cap protein OS=Porcine stool-associated circular virus 3 OX=1328032 PE=4 SV=1
MM1 pKa = 7.31SNKK4 pKa = 9.62VYY6 pKa = 9.98MATIPHH12 pKa = 5.92SGITKK17 pKa = 9.51RR18 pKa = 11.84AFFKK22 pKa = 10.21MIRR25 pKa = 11.84DD26 pKa = 3.89FDD28 pKa = 3.54IHH30 pKa = 6.45KK31 pKa = 9.56WVYY34 pKa = 9.99AVEE37 pKa = 4.48RR38 pKa = 11.84GHH40 pKa = 6.81GGYY43 pKa = 9.96KK44 pKa = 10.28HH45 pKa = 5.34IQCRR49 pKa = 11.84FRR51 pKa = 11.84TNKK54 pKa = 9.93SFDD57 pKa = 3.87QIRR60 pKa = 11.84HH61 pKa = 5.26ALICGHH67 pKa = 6.57IEE69 pKa = 3.82EE70 pKa = 6.08ASDD73 pKa = 2.92QWEE76 pKa = 4.26YY77 pKa = 11.09EE78 pKa = 4.33KK79 pKa = 11.2KK80 pKa = 10.35DD81 pKa = 3.61GNYY84 pKa = 6.89MTSEE88 pKa = 4.37DD89 pKa = 3.33NHH91 pKa = 6.8EE92 pKa = 4.13ILKK95 pKa = 10.31LRR97 pKa = 11.84YY98 pKa = 9.26GKK100 pKa = 8.36LTKK103 pKa = 9.57VQEE106 pKa = 4.01WALVLLEE113 pKa = 4.21STNDD117 pKa = 3.34RR118 pKa = 11.84EE119 pKa = 4.41VVVWVDD125 pKa = 2.89KK126 pKa = 11.16DD127 pKa = 3.9GNSGKK132 pKa = 7.88TWLTAHH138 pKa = 6.28LWEE141 pKa = 5.42RR142 pKa = 11.84GQACYY147 pKa = 10.34CPPTLSTPKK156 pKa = 10.39EE157 pKa = 4.13LISWVHH163 pKa = 5.06SAYY166 pKa = 10.54NHH168 pKa = 5.91EE169 pKa = 4.56PYY171 pKa = 10.23IIIDD175 pKa = 3.93IPRR178 pKa = 11.84TWKK181 pKa = 9.93WDD183 pKa = 3.57DD184 pKa = 3.52ALYY187 pKa = 9.47TAIEE191 pKa = 4.37TIKK194 pKa = 10.95DD195 pKa = 3.31GLVYY199 pKa = 10.39DD200 pKa = 4.44PRR202 pKa = 11.84YY203 pKa = 8.29SAHH206 pKa = 5.72MRR208 pKa = 11.84NIRR211 pKa = 11.84GVKK214 pKa = 8.4VMCMTNTEE222 pKa = 4.31PKK224 pKa = 9.18TSKK227 pKa = 10.33LSEE230 pKa = 4.58DD231 pKa = 3.03RR232 pKa = 11.84WVMYY236 pKa = 10.33RR237 pKa = 11.84PSNLFEE243 pKa = 3.98TAEE246 pKa = 4.15LRR248 pKa = 11.84NSRR251 pKa = 11.84GGHH254 pKa = 6.1PPGSHH259 pKa = 5.37QRR261 pKa = 11.84CEE263 pKa = 3.97PPPPVRR269 pKa = 4.95

Molecular weight: 31.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

568

269

299

284.0

32.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.754 ± 0.057

1.408 ± 0.594

5.634 ± 0.042

4.93 ± 1.542

4.754 ± 1.823

7.218 ± 1.186

2.641 ± 1.584

5.106 ± 0.877

6.338 ± 0.524

6.514 ± 0.409

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.368

4.577 ± 0.353

6.162 ± 0.423

3.521 ± 0.933

4.93 ± 1.542

6.69 ± 0.536

7.218 ± 0.649

7.394 ± 1.314

2.289 ± 1.032

5.458 ± 0.452
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski