Algoriphagus kandeliae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cyclobacteriaceae; Algoriphagus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3350 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9QZC7|A0A4Y9QZC7_9BACT Metal ABC transporter permease OS=Algoriphagus kandeliae OX=2562278 GN=E4S40_02985 PE=3 SV=1
MM1 pKa = 7.66AFLIFSCDD9 pKa = 3.27KK10 pKa = 10.36EE11 pKa = 4.69VEE13 pKa = 4.45VPGGGAAFDD22 pKa = 4.68CNQLSYY28 pKa = 11.45SDD30 pKa = 3.7TIYY33 pKa = 10.93YY34 pKa = 10.03ISATQSVLVSPQTNLEE50 pKa = 4.07GQGTFEE56 pKa = 4.32VSPDD60 pKa = 3.03GLAIDD65 pKa = 4.39TNTGVIDD72 pKa = 3.8VNASEE77 pKa = 4.62TGLKK81 pKa = 10.38YY82 pKa = 10.68KK83 pKa = 9.37ITFTPTGSNQVCEE96 pKa = 4.28TFVTIGGVNYY106 pKa = 10.54LDD108 pKa = 4.61GIYY111 pKa = 10.85VLDD114 pKa = 4.12QNEE117 pKa = 4.29TLAGPIYY124 pKa = 10.59NGVPGLPLPCPDD136 pKa = 5.38DD137 pKa = 5.8DD138 pKa = 6.08SDD140 pKa = 5.75DD141 pKa = 4.19GSSDD145 pKa = 5.03DD146 pKa = 4.88DD147 pKa = 3.95SCDD150 pKa = 3.51FDD152 pKa = 4.01VEE154 pKa = 4.4NPSGILLEE162 pKa = 4.51DD163 pKa = 3.64LGFEE167 pKa = 4.09ISSKK171 pKa = 10.98GVFDD175 pKa = 4.91LLEE178 pKa = 4.25TVEE181 pKa = 4.56NGTFGAIPVNGEE193 pKa = 3.94SLDD196 pKa = 3.55VEE198 pKa = 5.07LYY200 pKa = 10.92YY201 pKa = 10.82KK202 pKa = 10.92LPDD205 pKa = 3.75LSSNALNSIPLRR217 pKa = 11.84FFYY220 pKa = 11.18YY221 pKa = 9.28EE222 pKa = 4.07TVADD226 pKa = 4.15IPQALLDD233 pKa = 4.99DD234 pKa = 4.48IEE236 pKa = 4.67EE237 pKa = 4.33KK238 pKa = 10.76SDD240 pKa = 4.28LINEE244 pKa = 4.25SRR246 pKa = 11.84SGNSLNFRR254 pKa = 11.84VMNEE258 pKa = 3.64TRR260 pKa = 11.84PRR262 pKa = 11.84RR263 pKa = 11.84VPRR266 pKa = 11.84PPFIVIVARR275 pKa = 11.84LEE277 pKa = 3.93

Molecular weight: 30.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9QI78|A0A4Y9QI78_9BACT Ligase-associated DNA damage response exonuclease OS=Algoriphagus kandeliae OX=2562278 GN=E4S40_16250 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.77RR12 pKa = 11.84KK13 pKa = 8.25NKK15 pKa = 9.37HH16 pKa = 4.12GFRR19 pKa = 11.84EE20 pKa = 4.15RR21 pKa = 11.84MSTANGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 10.53ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.51KK41 pKa = 10.08LTVSSEE47 pKa = 3.96KK48 pKa = 9.88TLKK51 pKa = 10.51KK52 pKa = 10.72

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3350

0

3350

1196724

38

3576

357.2

40.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.578 ± 0.043

0.633 ± 0.013

5.368 ± 0.031

7.343 ± 0.039

5.491 ± 0.032

7.164 ± 0.036

1.817 ± 0.021

7.285 ± 0.036

6.547 ± 0.046

9.895 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.299 ± 0.025

4.893 ± 0.038

4.124 ± 0.026

3.928 ± 0.029

4.171 ± 0.03

6.55 ± 0.031

4.834 ± 0.032

6.085 ± 0.032

1.323 ± 0.018

3.67 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski