Erwinia phage ENT90

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Entnonagintavirus; Erwinia virus ENT90

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F1BUQ6|F1BUQ6_9CAUD Phage head completion protein OS=Erwinia phage ENT90 OX=947843 PE=4 SV=1
MM1 pKa = 8.05RR2 pKa = 11.84IYY4 pKa = 10.48AQQGDD9 pKa = 4.52TVDD12 pKa = 4.59EE13 pKa = 4.18ICFRR17 pKa = 11.84YY18 pKa = 9.93YY19 pKa = 11.15GRR21 pKa = 11.84TQQVVEE27 pKa = 4.05QVYY30 pKa = 10.07AANPGLAEE38 pKa = 4.45SGPVLPHH45 pKa = 5.95GCEE48 pKa = 3.83VTLPPLPEE56 pKa = 4.59ASTGEE61 pKa = 4.46TVNLWDD67 pKa = 3.75

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F1BUQ4|F1BUQ4_9CAUD Uncharacterized protein OS=Erwinia phage ENT90 OX=947843 PE=4 SV=1
MM1 pKa = 7.68SLRR4 pKa = 11.84IQASITRR11 pKa = 11.84STRR14 pKa = 11.84ITSYY18 pKa = 10.69TRR20 pKa = 11.84FTAAWRR26 pKa = 11.84SLIWLCGVTPAVWPMPFVQTPALHH50 pKa = 6.64WYY52 pKa = 10.5GEE54 pKa = 4.09VRR56 pKa = 11.84QPSASAISCSPASDD70 pKa = 3.33VFRR73 pKa = 11.84VLNCGNRR80 pKa = 11.84AAMASTAATVQRR92 pKa = 11.84LAVWLKK98 pKa = 8.15VHH100 pKa = 6.86PLHH103 pKa = 7.31ALALQFIGFAAVMPVNKK120 pKa = 9.94KK121 pKa = 9.1GRR123 pKa = 11.84HH124 pKa = 4.35VNHH127 pKa = 6.96HH128 pKa = 6.78GDD130 pKa = 3.52HH131 pKa = 5.9GHH133 pKa = 7.31AGANHH138 pKa = 6.83KK139 pKa = 10.91GNLLRR144 pKa = 11.84RR145 pKa = 11.84VAAKK149 pKa = 9.4PHH151 pKa = 5.78QDD153 pKa = 3.16EE154 pKa = 4.45ADD156 pKa = 3.36QVVNQRR162 pKa = 11.84ADD164 pKa = 3.48FLHH167 pKa = 6.01VCC169 pKa = 4.48

Molecular weight: 18.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11021

62

825

186.8

20.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.623 ± 0.481

1.633 ± 0.22

4.618 ± 0.271

5.136 ± 0.359

3.566 ± 0.247

6.896 ± 0.312

2.032 ± 0.212

4.755 ± 0.189

4.165 ± 0.226

9.137 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.145

3.72 ± 0.245

4.745 ± 0.269

4.192 ± 0.235

7.522 ± 0.443

7.286 ± 0.495

5.989 ± 0.255

6.587 ± 0.34

1.388 ± 0.122

2.414 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski