Prevotella baroniae F0067

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella baroniae

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2480 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2NLW6|U2NLW6_9BACT Putative lipoprotein OS=Prevotella baroniae F0067 OX=1115809 GN=HMPREF9135_1221 PE=4 SV=1
MM1 pKa = 7.33SVFISKK7 pKa = 10.09EE8 pKa = 4.07LIPKK12 pKa = 9.56IEE14 pKa = 3.97RR15 pKa = 11.84YY16 pKa = 10.32YY17 pKa = 9.87NIKK20 pKa = 8.13MTDD23 pKa = 3.18PTEE26 pKa = 3.81EE27 pKa = 3.72VGYY30 pKa = 10.64GEE32 pKa = 3.84QAMAFEE38 pKa = 5.7DD39 pKa = 4.12VTDD42 pKa = 3.86SCSDD46 pKa = 3.61LEE48 pKa = 4.3DD49 pKa = 3.27WLEE52 pKa = 4.26NGDD55 pKa = 4.43VLTCYY60 pKa = 10.49EE61 pKa = 4.44DD62 pKa = 4.24ADD64 pKa = 4.04GQIHH68 pKa = 5.93YY69 pKa = 9.82KK70 pKa = 10.4VSVEE74 pKa = 3.82NGVFDD79 pKa = 4.82FVLDD83 pKa = 3.9KK84 pKa = 11.54EE85 pKa = 4.39NVEE88 pKa = 4.17YY89 pKa = 10.57EE90 pKa = 4.48SYY92 pKa = 11.36LEE94 pKa = 5.39DD95 pKa = 4.72IKK97 pKa = 11.63SDD99 pKa = 3.8LNNLFSNASVEE110 pKa = 4.56DD111 pKa = 4.35FRR113 pKa = 11.84TLCQNLQDD121 pKa = 3.56YY122 pKa = 11.29DD123 pKa = 3.97EE124 pKa = 4.62YY125 pKa = 11.29KK126 pKa = 10.47KK127 pKa = 10.7SHH129 pKa = 6.77EE130 pKa = 4.16DD131 pKa = 3.48VIDD134 pKa = 3.91FDD136 pKa = 6.24LIDD139 pKa = 4.16KK140 pKa = 10.31EE141 pKa = 4.52DD142 pKa = 3.66YY143 pKa = 10.76EE144 pKa = 5.51SIVKK148 pKa = 9.62EE149 pKa = 4.37AITEE153 pKa = 4.18YY154 pKa = 10.46IEE156 pKa = 4.73KK157 pKa = 10.47NDD159 pKa = 3.11IKK161 pKa = 11.36AEE163 pKa = 4.01IKK165 pKa = 10.61GLSADD170 pKa = 4.04DD171 pKa = 3.76VVMDD175 pKa = 4.5GDD177 pKa = 4.02NSFTYY182 pKa = 10.04KK183 pKa = 10.72GEE185 pKa = 4.01EE186 pKa = 3.94YY187 pKa = 10.54QGFDD191 pKa = 3.71SSDD194 pKa = 3.27GGDD197 pKa = 4.01FDD199 pKa = 4.93CTSCEE204 pKa = 4.01NFDD207 pKa = 5.01LIYY210 pKa = 10.74EE211 pKa = 4.78AVQEE215 pKa = 4.37ANCEE219 pKa = 4.17DD220 pKa = 4.11KK221 pKa = 11.5EE222 pKa = 4.21EE223 pKa = 4.02LTMYY227 pKa = 10.16LCGMNFVYY235 pKa = 10.56KK236 pKa = 10.78NLVDD240 pKa = 3.55DD241 pKa = 4.27VMYY244 pKa = 10.94KK245 pKa = 10.29FYY247 pKa = 11.04FKK249 pKa = 11.09

Molecular weight: 28.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2NLX0|U2NLX0_9BACT Acyltransferase OS=Prevotella baroniae F0067 OX=1115809 GN=HMPREF9135_1243 PE=4 SV=1
MM1 pKa = 6.72EE2 pKa = 4.32TVVTLMMLLTVGCFLLKK19 pKa = 8.79QTFMGVRR26 pKa = 11.84QILVTAVVVMMFVALMWPFAITQSKK51 pKa = 6.73TQIASWLSNPQLMLDD66 pKa = 3.77VAVLLSVDD74 pKa = 3.23IALEE78 pKa = 3.72IAFCVTDD85 pKa = 3.19VDD87 pKa = 3.85VRR89 pKa = 11.84TSRR92 pKa = 11.84KK93 pKa = 7.3VSRR96 pKa = 11.84RR97 pKa = 11.84KK98 pKa = 10.29RR99 pKa = 11.84MWFRR103 pKa = 11.84FLRR106 pKa = 11.84HH107 pKa = 5.71FPGLLVFPVFFAVLVWAIFALPGVDD132 pKa = 3.96FALVGWSLGVALLFVLPLLAYY153 pKa = 10.2LLRR156 pKa = 11.84RR157 pKa = 11.84LVPEE161 pKa = 3.8EE162 pKa = 3.86EE163 pKa = 4.39LRR165 pKa = 11.84LEE167 pKa = 5.39LLFLCNLLLGGLGVIATVNGTTAVKK192 pKa = 10.39GVSQVNYY199 pKa = 9.93AALGAMAALILVVGGVGFVARR220 pKa = 11.84RR221 pKa = 11.84VRR223 pKa = 11.84QKK225 pKa = 10.57RR226 pKa = 11.84AVARR230 pKa = 11.84LQRR233 pKa = 11.84KK234 pKa = 8.3QYY236 pKa = 10.76GRR238 pKa = 11.84TACPSHH244 pKa = 6.95PP245 pKa = 3.81

Molecular weight: 27.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2480

0

2480

896784

37

2721

361.6

40.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.934 ± 0.045

1.217 ± 0.018

5.877 ± 0.034

5.79 ± 0.044

4.434 ± 0.028

7.189 ± 0.043

2.154 ± 0.023

5.736 ± 0.042

6.129 ± 0.041

9.12 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.88 ± 0.022

4.773 ± 0.041

3.763 ± 0.025

3.631 ± 0.027

5.504 ± 0.04

5.838 ± 0.04

5.722 ± 0.036

6.787 ± 0.038

1.281 ± 0.018

4.242 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski