Brenneria alni

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Pectobacteriaceae; Brenneria

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3626 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A421DSC5|A0A421DSC5_9GAMM TPR_REGION domain-containing protein OS=Brenneria alni OX=71656 GN=BIY29_03140 PE=4 SV=1
MM1 pKa = 7.54GFSQAISGLNAASSNLDD18 pKa = 3.36VIGNNIANSEE28 pKa = 4.32TTGFKK33 pKa = 10.31AASVSFADD41 pKa = 4.24IFASSQVGLGVKK53 pKa = 9.33VASVSQDD60 pKa = 3.17FGDD63 pKa = 3.92GTVEE67 pKa = 3.99STDD70 pKa = 3.22RR71 pKa = 11.84GLDD74 pKa = 3.28VAIAGTGFFRR84 pKa = 11.84MTDD87 pKa = 3.49EE88 pKa = 4.58NGSVYY93 pKa = 10.45YY94 pKa = 10.51SRR96 pKa = 11.84NGEE99 pKa = 3.93LSLDD103 pKa = 3.69SEE105 pKa = 4.66RR106 pKa = 11.84NIVNSSGYY114 pKa = 10.48YY115 pKa = 7.39LTGYY119 pKa = 9.76AATGTPPTINTGAEE133 pKa = 3.77PTAINIPSGDD143 pKa = 3.19IAANATNSASMVLNLNSSADD163 pKa = 3.93TIPAGTTFDD172 pKa = 3.48ATDD175 pKa = 3.32ASTYY179 pKa = 10.35SYY181 pKa = 11.4SRR183 pKa = 11.84TLTTYY188 pKa = 10.65DD189 pKa = 3.47SQGNTHH195 pKa = 6.11NVNLYY200 pKa = 9.21FAKK203 pKa = 10.4TNDD206 pKa = 3.64NTWNVYY212 pKa = 10.07GVDD215 pKa = 3.74SSDD218 pKa = 3.9AAAEE222 pKa = 3.98PQTLGTLTFDD232 pKa = 3.65GSGMLTGTTEE242 pKa = 4.73LNVATNGINGSDD254 pKa = 3.96DD255 pKa = 3.24NTFAISFTGTTQQSASASTISSSTQDD281 pKa = 2.85GYY283 pKa = 10.98MAGSLSSYY291 pKa = 9.23TINSDD296 pKa = 3.27GTITGTYY303 pKa = 10.7SNGQTQLLGQIVMASFANPQGLKK326 pKa = 10.75AEE328 pKa = 4.58GDD330 pKa = 4.19NVWSSTASSGAAIVGTAGVGVFGSLTSGALEE361 pKa = 4.07SSNVDD366 pKa = 3.65LSAEE370 pKa = 4.11LVNMIVAQRR379 pKa = 11.84NYY381 pKa = 10.23QSNAQTIKK389 pKa = 9.47TQDD392 pKa = 3.73AILNTLVNLRR402 pKa = 4.14

Molecular weight: 41.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A421DQ17|A0A421DQ17_9GAMM EscR/YscR/HrcR family type III secretion system export apparatus protein OS=Brenneria alni OX=71656 GN=BIY29_07325 PE=3 SV=1
MM1 pKa = 7.06LTIAAHH7 pKa = 6.22CWLCRR12 pKa = 11.84LPLYY16 pKa = 10.28HH17 pKa = 6.84SRR19 pKa = 11.84HH20 pKa = 6.4GICSFCQRR28 pKa = 11.84HH29 pKa = 5.59LPQRR33 pKa = 11.84PLCCPRR39 pKa = 11.84CGLLSGDD46 pKa = 3.98PTRR49 pKa = 11.84QCGRR53 pKa = 11.84CLKK56 pKa = 10.48NPPPWQSITFVSDD69 pKa = 2.96YY70 pKa = 11.02VPPLNTLMKK79 pKa = 10.19QFKK82 pKa = 10.55FNGKK86 pKa = 7.55TEE88 pKa = 4.23LAPVLARR95 pKa = 11.84QILLRR100 pKa = 11.84WLTTYY105 pKa = 10.64RR106 pKa = 11.84EE107 pKa = 4.39HH108 pKa = 7.34ALSAPPHH115 pKa = 6.7LFRR118 pKa = 11.84PDD120 pKa = 3.32QLFTVPLHH128 pKa = 6.05QRR130 pKa = 11.84RR131 pKa = 11.84HH132 pKa = 4.09WSRR135 pKa = 11.84GFNQTEE141 pKa = 3.84LLARR145 pKa = 11.84CLTRR149 pKa = 11.84WLRR152 pKa = 11.84CDD154 pKa = 3.5YY155 pKa = 10.45QPRR158 pKa = 11.84AIARR162 pKa = 11.84IRR164 pKa = 11.84QTRR167 pKa = 11.84IQQKK171 pKa = 9.48LSASARR177 pKa = 11.84RR178 pKa = 11.84KK179 pKa = 8.6NLRR182 pKa = 11.84GAFSCNTALTGKK194 pKa = 9.85QVVLLDD200 pKa = 3.96DD201 pKa = 4.01VVTTGSTAAEE211 pKa = 3.99ISRR214 pKa = 11.84VLLAQGAAGVQVWCLCRR231 pKa = 11.84TLL233 pKa = 4.39

Molecular weight: 26.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3626

0

3626

1149954

38

3036

317.1

35.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.555 ± 0.049

1.057 ± 0.016

5.32 ± 0.032

5.607 ± 0.039

3.69 ± 0.029

7.263 ± 0.039

2.264 ± 0.021

6.024 ± 0.038

4.093 ± 0.034

11.029 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.021

3.775 ± 0.028

4.452 ± 0.028

4.831 ± 0.037

5.933 ± 0.04

6.163 ± 0.035

5.271 ± 0.032

6.868 ± 0.033

1.366 ± 0.018

2.846 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski