Amino acid dipepetide frequency for Tenacibaculum phage PTm1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.045AlaAla: 0.045 ± 0.022
0.419AlaCys: 0.419 ± 0.081
2.247AlaAsp: 2.247 ± 0.167
3.07AlaGlu: 3.07 ± 0.279
1.752AlaPhe: 1.752 ± 0.163
2.801AlaGly: 2.801 ± 0.326
0.719AlaHis: 0.719 ± 0.101
2.801AlaIle: 2.801 ± 0.218
3.969AlaLys: 3.969 ± 0.274
4.029AlaLeu: 4.029 ± 0.313
0.929AlaMet: 0.929 ± 0.132
2.995AlaAsn: 2.995 ± 0.205
1.213AlaPro: 1.213 ± 0.169
1.498AlaGln: 1.498 ± 0.142
1.648AlaArg: 1.648 ± 0.165
3.43AlaSer: 3.43 ± 0.256
3.31AlaThr: 3.31 ± 0.315
2.981AlaVal: 2.981 ± 0.234
0.33AlaTrp: 0.33 ± 0.073
2.292AlaTyr: 2.292 ± 0.163
0.0AlaXaa: 0.0 ± 0.0
Cys
0.315CysAla: 0.315 ± 0.065
0.075CysCys: 0.075 ± 0.033
0.899CysAsp: 0.899 ± 0.104
0.719CysGlu: 0.719 ± 0.103
0.479CysPhe: 0.479 ± 0.085
0.659CysGly: 0.659 ± 0.09
0.195CysHis: 0.195 ± 0.056
0.884CysIle: 0.884 ± 0.13
0.899CysLys: 0.899 ± 0.158
0.659CysLeu: 0.659 ± 0.095
0.195CysMet: 0.195 ± 0.056
0.554CysAsn: 0.554 ± 0.088
0.359CysPro: 0.359 ± 0.072
0.18CysGln: 0.18 ± 0.053
0.3CysArg: 0.3 ± 0.068
0.644CysSer: 0.644 ± 0.09
0.569CysThr: 0.569 ± 0.075
0.659CysVal: 0.659 ± 0.115
0.135CysTrp: 0.135 ± 0.048
0.419CysTyr: 0.419 ± 0.067
0.0CysXaa: 0.0 ± 0.0
Asp
3.1AspAla: 3.1 ± 0.246
0.704AspCys: 0.704 ± 0.122
3.939AspAsp: 3.939 ± 0.243
4.403AspGlu: 4.403 ± 0.293
3.505AspPhe: 3.505 ± 0.246
4.284AspGly: 4.284 ± 0.256
0.704AspHis: 0.704 ± 0.085
5.991AspIle: 5.991 ± 0.265
5.242AspLys: 5.242 ± 0.32
5.512AspLeu: 5.512 ± 0.317
1.393AspMet: 1.393 ± 0.132
3.669AspAsn: 3.669 ± 0.245
1.378AspPro: 1.378 ± 0.162
0.914AspGln: 0.914 ± 0.129
2.217AspArg: 2.217 ± 0.184
4.358AspSer: 4.358 ± 0.234
4.014AspThr: 4.014 ± 0.227
4.643AspVal: 4.643 ± 0.299
0.644AspTrp: 0.644 ± 0.109
3.025AspTyr: 3.025 ± 0.221
0.0AspXaa: 0.0 ± 0.0
Glu
2.681GluAla: 2.681 ± 0.241
0.809GluCys: 0.809 ± 0.115
3.475GluAsp: 3.475 ± 0.229
3.774GluGlu: 3.774 ± 0.285
3.37GluPhe: 3.37 ± 0.243
1.662GluGly: 1.662 ± 0.17
1.483GluHis: 1.483 ± 0.166
5.587GluIle: 5.587 ± 0.334
4.014GluLys: 4.014 ± 0.246
7.668GluLeu: 7.668 ± 0.434
1.498GluMet: 1.498 ± 0.157
4.463GluAsn: 4.463 ± 0.309
1.528GluPro: 1.528 ± 0.169
3.01GluGln: 3.01 ± 0.209
2.591GluArg: 2.591 ± 0.219
4.568GluSer: 4.568 ± 0.359
3.46GluThr: 3.46 ± 0.243
4.568GluVal: 4.568 ± 0.214
0.689GluTrp: 0.689 ± 0.108
3.744GluTyr: 3.744 ± 0.213
0.0GluXaa: 0.0 ± 0.0
Phe
1.812PheAla: 1.812 ± 0.165
0.539PheCys: 0.539 ± 0.087
3.954PheAsp: 3.954 ± 0.217
3.415PheGlu: 3.415 ± 0.271
1.902PhePhe: 1.902 ± 0.171
2.876PheGly: 2.876 ± 0.199
0.629PheHis: 0.629 ± 0.083
3.535PheIle: 3.535 ± 0.217
4.583PheLys: 4.583 ± 0.289
2.741PheLeu: 2.741 ± 0.203
1.123PheMet: 1.123 ± 0.131
4.134PheAsn: 4.134 ± 0.257
1.138PhePro: 1.138 ± 0.103
0.929PheGln: 0.929 ± 0.116
2.022PheArg: 2.022 ± 0.184
3.04PheSer: 3.04 ± 0.203
2.951PheThr: 2.951 ± 0.23
2.891PheVal: 2.891 ± 0.182
0.434PheTrp: 0.434 ± 0.083
1.722PheTyr: 1.722 ± 0.215
0.0PheXaa: 0.0 ± 0.0
Gly
2.292GlyAla: 2.292 ± 0.317
0.509GlyCys: 0.509 ± 0.081
3.385GlyAsp: 3.385 ± 0.226
2.816GlyGlu: 2.816 ± 0.201
2.172GlyPhe: 2.172 ± 0.166
2.816GlyGly: 2.816 ± 0.256
1.093GlyHis: 1.093 ± 0.163
3.864GlyIle: 3.864 ± 0.25
4.179GlyLys: 4.179 ± 0.289
3.819GlyLeu: 3.819 ± 0.26
1.588GlyMet: 1.588 ± 0.166
3.729GlyAsn: 3.729 ± 0.291
0.18GlyPro: 0.18 ± 0.052
1.468GlyGln: 1.468 ± 0.194
1.767GlyArg: 1.767 ± 0.157
3.61GlySer: 3.61 ± 0.251
3.984GlyThr: 3.984 ± 0.313
3.684GlyVal: 3.684 ± 0.235
0.569GlyTrp: 0.569 ± 0.091
2.636GlyTyr: 2.636 ± 0.281
0.0GlyXaa: 0.0 ± 0.0
His
0.734HisAla: 0.734 ± 0.101
0.225HisCys: 0.225 ± 0.054
1.213HisAsp: 1.213 ± 0.134
0.914HisGlu: 0.914 ± 0.114
0.869HisPhe: 0.869 ± 0.101
1.153HisGly: 1.153 ± 0.128
0.554HisHis: 0.554 ± 0.092
1.887HisIle: 1.887 ± 0.198
1.648HisLys: 1.648 ± 0.158
1.378HisLeu: 1.378 ± 0.157
0.404HisMet: 0.404 ± 0.077
1.423HisAsn: 1.423 ± 0.146
0.569HisPro: 0.569 ± 0.085
0.539HisGln: 0.539 ± 0.084
0.989HisArg: 0.989 ± 0.116
1.393HisSer: 1.393 ± 0.148
1.423HisThr: 1.423 ± 0.165
0.944HisVal: 0.944 ± 0.111
0.15HisTrp: 0.15 ± 0.045
0.854HisTyr: 0.854 ± 0.116
0.0HisXaa: 0.0 ± 0.0
Ile
3.13IleAla: 3.13 ± 0.245
0.689IleCys: 0.689 ± 0.099
5.751IleAsp: 5.751 ± 0.288
5.901IleGlu: 5.901 ± 0.341
3.1IlePhe: 3.1 ± 0.188
3.714IleGly: 3.714 ± 0.35
1.513IleHis: 1.513 ± 0.138
5.676IleIle: 5.676 ± 0.378
7.998IleLys: 7.998 ± 0.36
6.246IleLeu: 6.246 ± 0.321
1.707IleMet: 1.707 ± 0.176
5.811IleAsn: 5.811 ± 0.327
2.906IlePro: 2.906 ± 0.184
3.19IleGln: 3.19 ± 0.229
2.696IleArg: 2.696 ± 0.213
5.437IleSer: 5.437 ± 0.282
5.002IleThr: 5.002 ± 0.261
4.029IleVal: 4.029 ± 0.268
0.734IleTrp: 0.734 ± 0.11
3.055IleTyr: 3.055 ± 0.252
0.0IleXaa: 0.0 ± 0.0
Lys
3.774LysAla: 3.774 ± 0.256
0.854LysCys: 0.854 ± 0.116
5.092LysAsp: 5.092 ± 0.312
6.201LysGlu: 6.201 ± 0.353
4.029LysPhe: 4.029 ± 0.256
3.879LysGly: 3.879 ± 0.279
2.082LysHis: 2.082 ± 0.188
6.59LysIle: 6.59 ± 0.323
7.384LysLys: 7.384 ± 0.533
8.537LysLeu: 8.537 ± 0.383
2.307LysMet: 2.307 ± 0.217
6.38LysAsn: 6.38 ± 0.331
2.546LysPro: 2.546 ± 0.222
3.729LysGln: 3.729 ± 0.224
3.415LysArg: 3.415 ± 0.22
6.156LysSer: 6.156 ± 0.343
5.572LysThr: 5.572 ± 0.345
4.928LysVal: 4.928 ± 0.273
1.108LysTrp: 1.108 ± 0.137
5.272LysTyr: 5.272 ± 0.339
0.0LysXaa: 0.0 ± 0.0
Leu
3.235LeuAla: 3.235 ± 0.237
0.809LeuCys: 0.809 ± 0.094
5.092LeuAsp: 5.092 ± 0.245
5.002LeuGlu: 5.002 ± 0.326
3.43LeuPhe: 3.43 ± 0.231
3.924LeuGly: 3.924 ± 0.268
1.752LeuHis: 1.752 ± 0.157
5.242LeuIle: 5.242 ± 0.268
8.312LeuLys: 8.312 ± 0.446
6.201LeuLeu: 6.201 ± 0.316
2.127LeuMet: 2.127 ± 0.196
6.38LeuAsn: 6.38 ± 0.36
2.861LeuPro: 2.861 ± 0.242
2.891LeuGln: 2.891 ± 0.18
3.669LeuArg: 3.669 ± 0.216
6.71LeuSer: 6.71 ± 0.307
6.171LeuThr: 6.171 ± 0.337
5.092LeuVal: 5.092 ± 0.311
0.659LeuTrp: 0.659 ± 0.091
3.864LeuTyr: 3.864 ± 0.285
0.0LeuXaa: 0.0 ± 0.0
Met
1.063MetAla: 1.063 ± 0.124
0.21MetCys: 0.21 ± 0.05
1.363MetAsp: 1.363 ± 0.157
1.363MetGlu: 1.363 ± 0.15
1.483MetPhe: 1.483 ± 0.147
1.003MetGly: 1.003 ± 0.139
0.464MetHis: 0.464 ± 0.086
1.648MetIle: 1.648 ± 0.144
2.217MetLys: 2.217 ± 0.176
2.172MetLeu: 2.172 ± 0.178
0.554MetMet: 0.554 ± 0.087
1.498MetAsn: 1.498 ± 0.162
0.674MetPro: 0.674 ± 0.109
0.869MetGln: 0.869 ± 0.11
0.719MetArg: 0.719 ± 0.084
1.797MetSer: 1.797 ± 0.149
1.288MetThr: 1.288 ± 0.14
1.378MetVal: 1.378 ± 0.142
0.135MetTrp: 0.135 ± 0.049
0.989MetTyr: 0.989 ± 0.113
0.0MetXaa: 0.0 ± 0.0
Asn
3.31AsnAla: 3.31 ± 0.28
0.449AsnCys: 0.449 ± 0.092
4.074AsnAsp: 4.074 ± 0.268
5.032AsnGlu: 5.032 ± 0.343
2.876AsnPhe: 2.876 ± 0.178
4.224AsnGly: 4.224 ± 0.263
1.198AsnHis: 1.198 ± 0.163
6.485AsnIle: 6.485 ± 0.342
7.384AsnLys: 7.384 ± 0.373
5.197AsnLeu: 5.197 ± 0.314
1.707AsnMet: 1.707 ± 0.14
5.347AsnAsn: 5.347 ± 0.349
2.292AsnPro: 2.292 ± 0.191
2.247AsnGln: 2.247 ± 0.171
2.771AsnArg: 2.771 ± 0.176
4.568AsnSer: 4.568 ± 0.27
5.482AsnThr: 5.482 ± 0.504
4.343AsnVal: 4.343 ± 0.277
0.644AsnTrp: 0.644 ± 0.095
2.936AsnTyr: 2.936 ± 0.174
0.0AsnXaa: 0.0 ± 0.0
Pro
1.213ProAla: 1.213 ± 0.139
0.18ProCys: 0.18 ± 0.051
1.123ProAsp: 1.123 ± 0.128
1.198ProGlu: 1.198 ± 0.131
1.453ProPhe: 1.453 ± 0.154
0.344ProGly: 0.344 ± 0.084
0.674ProHis: 0.674 ± 0.115
2.336ProIle: 2.336 ± 0.164
2.831ProLys: 2.831 ± 0.248
2.307ProLeu: 2.307 ± 0.174
0.419ProMet: 0.419 ± 0.076
2.636ProAsn: 2.636 ± 0.188
0.599ProPro: 0.599 ± 0.088
0.824ProGln: 0.824 ± 0.105
0.824ProArg: 0.824 ± 0.121
2.322ProSer: 2.322 ± 0.182
2.621ProThr: 2.621 ± 0.224
1.752ProVal: 1.752 ± 0.218
0.33ProTrp: 0.33 ± 0.091
1.573ProTyr: 1.573 ± 0.162
0.0ProXaa: 0.0 ± 0.0
Gln
1.603GlnAla: 1.603 ± 0.18
0.27GlnCys: 0.27 ± 0.071
1.483GlnAsp: 1.483 ± 0.124
2.037GlnGlu: 2.037 ± 0.172
1.453GlnPhe: 1.453 ± 0.13
1.438GlnGly: 1.438 ± 0.153
0.599GlnHis: 0.599 ± 0.108
2.516GlnIle: 2.516 ± 0.182
2.786GlnLys: 2.786 ± 0.196
3.235GlnLeu: 3.235 ± 0.231
0.944GlnMet: 0.944 ± 0.115
2.262GlnAsn: 2.262 ± 0.186
1.168GlnPro: 1.168 ± 0.142
1.483GlnGln: 1.483 ± 0.16
1.318GlnArg: 1.318 ± 0.15
2.247GlnSer: 2.247 ± 0.226
2.202GlnThr: 2.202 ± 0.226
1.872GlnVal: 1.872 ± 0.205
0.315GlnTrp: 0.315 ± 0.067
1.737GlnTyr: 1.737 ± 0.138
0.0GlnXaa: 0.0 ± 0.0
Arg
1.543ArgAla: 1.543 ± 0.148
0.419ArgCys: 0.419 ± 0.075
2.022ArgAsp: 2.022 ± 0.181
2.786ArgGlu: 2.786 ± 0.219
1.857ArgPhe: 1.857 ± 0.186
1.722ArgGly: 1.722 ± 0.174
0.749ArgHis: 0.749 ± 0.114
2.846ArgIle: 2.846 ± 0.226
3.535ArgLys: 3.535 ± 0.279
3.28ArgLeu: 3.28 ± 0.244
1.003ArgMet: 1.003 ± 0.118
2.861ArgAsn: 2.861 ± 0.215
0.944ArgPro: 0.944 ± 0.116
1.063ArgGln: 1.063 ± 0.144
1.468ArgArg: 1.468 ± 0.154
1.977ArgSer: 1.977 ± 0.174
2.516ArgThr: 2.516 ± 0.186
2.576ArgVal: 2.576 ± 0.21
0.479ArgTrp: 0.479 ± 0.081
2.037ArgTyr: 2.037 ± 0.193
0.0ArgXaa: 0.0 ± 0.0
Ser
3.58SerAla: 3.58 ± 0.293
0.599SerCys: 0.599 ± 0.085
4.598SerAsp: 4.598 ± 0.249
4.928SerGlu: 4.928 ± 0.306
3.22SerPhe: 3.22 ± 0.222
3.744SerGly: 3.744 ± 0.295
1.243SerHis: 1.243 ± 0.143
5.841SerIle: 5.841 ± 0.291
6.306SerLys: 6.306 ± 0.373
5.242SerLeu: 5.242 ± 0.32
1.603SerMet: 1.603 ± 0.178
5.197SerAsn: 5.197 ± 0.349
1.857SerPro: 1.857 ± 0.18
1.977SerGln: 1.977 ± 0.218
2.232SerArg: 2.232 ± 0.191
4.328SerSer: 4.328 ± 0.295
4.763SerThr: 4.763 ± 0.317
4.808SerVal: 4.808 ± 0.275
0.719SerTrp: 0.719 ± 0.096
3.145SerTyr: 3.145 ± 0.226
0.0SerXaa: 0.0 ± 0.0
Thr
3.505ThrAla: 3.505 ± 0.321
0.539ThrCys: 0.539 ± 0.092
4.164ThrAsp: 4.164 ± 0.246
4.014ThrGlu: 4.014 ± 0.258
3.475ThrPhe: 3.475 ± 0.249
3.115ThrGly: 3.115 ± 0.44
1.333ThrHis: 1.333 ± 0.147
5.467ThrIle: 5.467 ± 0.319
6.35ThrLys: 6.35 ± 0.31
6.126ThrLeu: 6.126 ± 0.325
0.944ThrMet: 0.944 ± 0.124
5.077ThrAsn: 5.077 ± 0.322
2.232ThrPro: 2.232 ± 0.179
2.456ThrGln: 2.456 ± 0.242
2.007ThrArg: 2.007 ± 0.156
4.808ThrSer: 4.808 ± 0.276
4.883ThrThr: 4.883 ± 0.392
4.119ThrVal: 4.119 ± 0.408
0.569ThrTrp: 0.569 ± 0.091
2.891ThrTyr: 2.891 ± 0.152
0.0ThrXaa: 0.0 ± 0.0
Val
3.1ValAla: 3.1 ± 0.254
0.599ValCys: 0.599 ± 0.095
5.227ValAsp: 5.227 ± 0.29
3.924ValGlu: 3.924 ± 0.253
2.846ValPhe: 2.846 ± 0.237
3.325ValGly: 3.325 ± 0.274
1.123ValHis: 1.123 ± 0.136
4.718ValIle: 4.718 ± 0.282
4.958ValLys: 4.958 ± 0.272
4.299ValLeu: 4.299 ± 0.23
1.318ValMet: 1.318 ± 0.121
4.269ValAsn: 4.269 ± 0.232
1.752ValPro: 1.752 ± 0.179
1.947ValGln: 1.947 ± 0.202
2.681ValArg: 2.681 ± 0.218
4.987ValSer: 4.987 ± 0.289
4.119ValThr: 4.119 ± 0.311
4.373ValVal: 4.373 ± 0.283
0.479ValTrp: 0.479 ± 0.085
2.726ValTyr: 2.726 ± 0.209
0.0ValXaa: 0.0 ± 0.0
Trp
0.344TrpAla: 0.344 ± 0.073
0.285TrpCys: 0.285 ± 0.064
0.644TrpAsp: 0.644 ± 0.095
0.374TrpGlu: 0.374 ± 0.077
0.674TrpPhe: 0.674 ± 0.092
0.389TrpGly: 0.389 ± 0.095
0.21TrpHis: 0.21 ± 0.061
0.704TrpIle: 0.704 ± 0.098
0.854TrpLys: 0.854 ± 0.104
1.033TrpLeu: 1.033 ± 0.122
0.255TrpMet: 0.255 ± 0.055
0.689TrpAsn: 0.689 ± 0.111
0.0TrpPro: 0.0 ± 0.0
0.479TrpGln: 0.479 ± 0.098
0.315TrpArg: 0.315 ± 0.079
0.659TrpSer: 0.659 ± 0.107
0.539TrpThr: 0.539 ± 0.095
0.569TrpVal: 0.569 ± 0.097
0.09TrpTrp: 0.09 ± 0.033
0.689TrpTyr: 0.689 ± 0.106
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.097TyrAla: 2.097 ± 0.199
0.614TyrCys: 0.614 ± 0.094
3.909TyrAsp: 3.909 ± 0.251
2.771TyrGlu: 2.771 ± 0.234
2.426TyrPhe: 2.426 ± 0.202
3.085TyrGly: 3.085 ± 0.203
0.944TyrHis: 0.944 ± 0.149
3.669TyrIle: 3.669 ± 0.222
4.388TyrLys: 4.388 ± 0.242
3.744TyrLeu: 3.744 ± 0.304
0.854TyrMet: 0.854 ± 0.092
3.19TyrAsn: 3.19 ± 0.249
1.363TyrPro: 1.363 ± 0.152
1.273TyrGln: 1.273 ± 0.124
2.037TyrArg: 2.037 ± 0.184
2.891TyrSer: 2.891 ± 0.24
3.205TyrThr: 3.205 ± 0.247
2.561TyrVal: 2.561 ± 0.204
0.584TyrTrp: 0.584 ± 0.105
2.277TyrTyr: 2.277 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 308 proteins (66768 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski