Burkholderiales bacterium GJ-E10

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; unclassified Burkholderiales

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2955 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1H0I7|A0A0A1H0I7_9BURK Uncharacterized protein OS=Burkholderiales bacterium GJ-E10 OX=1469502 GN=E1O_06300 PE=4 SV=1
MM1 pKa = 7.91TITQATQNAAINWQSFNIGSNQSVTFVQPNASAVALNRR39 pKa = 11.84VLGPDD44 pKa = 3.91PSNIWGTLDD53 pKa = 3.64ANGKK57 pKa = 9.41VFLINPNGILFGKK70 pKa = 9.54GASINVGGLVASTLGLSDD88 pKa = 4.33RR89 pKa = 11.84DD90 pKa = 3.82FLAGNYY96 pKa = 9.1RR97 pKa = 11.84FSGASAAAVQNQGSITADD115 pKa = 2.97GGYY118 pKa = 10.08VALLGANVSNQGVIAARR135 pKa = 11.84LGTVALAGGNAVTLDD150 pKa = 3.51VAGDD154 pKa = 3.83GLLSVAVDD162 pKa = 3.39QGAVHH167 pKa = 7.22ALVQNGGLIQADD179 pKa = 3.92GGTVLLTTQAAGNLLSTAVNDD200 pKa = 3.67SGIIEE205 pKa = 4.4AQTVEE210 pKa = 4.32SHH212 pKa = 6.32NGTIRR217 pKa = 11.84LLGDD221 pKa = 3.53MQSGTVTVSGLLDD234 pKa = 3.77ASAPNGGNGGNIEE247 pKa = 4.14TSAAMLDD254 pKa = 3.5ISPTVAITTAAPSGTAGTWLLDD276 pKa = 3.92PYY278 pKa = 11.25NVTISTAADD287 pKa = 3.54SNTSAFTATASGANINTTTLQNALATTSVTVTTGSSGTEE326 pKa = 3.67NGDD329 pKa = 3.26ITVSAPISWSANTNLTLAAAGSIYY353 pKa = 10.79LNAGIANSGGGSIEE367 pKa = 3.95AKK369 pKa = 10.05AAGNIIQAGVGITTQGGNVVYY390 pKa = 9.56WANDD394 pKa = 3.39AGNGGYY400 pKa = 9.56VEE402 pKa = 4.43LQSGSSITTNGGGLWIGGGSGTAQWTPYY430 pKa = 10.2AGASAITVGNGYY442 pKa = 10.61AVGNATLTASGSTNFEE458 pKa = 4.0SGVIVAGPNSGAPTVSLDD476 pKa = 3.98TISSGSTDD484 pKa = 2.87GSISINGATPATAISGDD501 pKa = 3.44NVQGVTIYY509 pKa = 10.4GANVSSGRR517 pKa = 11.84GTLTINGQGSTTAGSPGPVQAIEE540 pKa = 4.59FGNSTLSSTSGAISLTGDD558 pKa = 3.0ASGGSGSNQFLGIHH572 pKa = 6.71WDD574 pKa = 3.46PSGTVASTTGAVNITGYY591 pKa = 10.97SDD593 pKa = 4.24INGQPWSSQDD603 pKa = 2.98VGLAMQGTLGGAGEE617 pKa = 4.79HH618 pKa = 6.85GNITLQTDD626 pKa = 3.61VFLFDD631 pKa = 3.99SGTDD635 pKa = 3.63LVQSTGGLTILPKK648 pKa = 10.5AGEE651 pKa = 4.22GLVVSASTPSTNSAPEE667 pKa = 3.77TLMPIWFNTSGTYY680 pKa = 8.77FVPGFSGITLGSSGSTGAVTVSSPLSVADD709 pKa = 3.96PLTINGSTVTVGANLTNTALNSAILVQATGDD740 pKa = 3.37ITQTASAAVTTNGGSVTYY758 pKa = 10.29DD759 pKa = 3.21SNSANLGSGNIWLQGTGTSGASINTNGGNITLSGGSNVATGYY801 pKa = 9.05ATGDD805 pKa = 3.18AALNGNGVSLDD816 pKa = 3.73TVNLFSGGGNIVIRR830 pKa = 11.84GKK832 pKa = 10.81SSTTNPSFSTSEE844 pKa = 4.02CSPCNTNDD852 pKa = 4.26GIHH855 pKa = 6.31MYY857 pKa = 10.81GSDD860 pKa = 4.15VINAGSGTIDD870 pKa = 3.58LEE872 pKa = 4.62GVAVGNSSSQYY883 pKa = 9.81SNGIEE888 pKa = 4.06TSITGYY894 pKa = 8.63TRR896 pKa = 11.84ILSSATNATAINLVGDD912 pKa = 4.46ASASTSSSDD921 pKa = 2.94EE922 pKa = 3.72WGVFLWGGNNFGIVVAATGTGGGVSISGKK951 pKa = 10.2GGNFTGKK958 pKa = 10.28AGGTHH963 pKa = 6.07TEE965 pKa = 3.71PNAYY969 pKa = 9.59VLAASGPISLTGTAGALSTTYY990 pKa = 11.37GDD992 pKa = 3.1VDD994 pKa = 4.42LNSTVGFVSTLPSGFGIASPVTSSSSNISITADD1027 pKa = 3.15SFKK1030 pKa = 10.41TDD1032 pKa = 3.22VVFGSGTFTSSAVQSSGTLSIAPRR1056 pKa = 11.84SPGKK1060 pKa = 10.38ALAVQTTPPSGTPLWINPTTMFGSSGLFKK1089 pKa = 10.46TGFSSFVFGSASTGTVTLDD1108 pKa = 2.83NYY1110 pKa = 10.67TFDD1113 pKa = 3.89NVTTVDD1119 pKa = 3.22TGANAILGTISIPNHH1134 pKa = 5.99ALTVNITGSGSVTDD1148 pKa = 3.57TGVIDD1153 pKa = 4.14VSSLALDD1160 pKa = 4.04AASSAVTLDD1169 pKa = 3.27NTGNAIGTLAADD1181 pKa = 3.9VASLSLDD1188 pKa = 3.22NATGLTVGSVAALNGVSATGGIDD1211 pKa = 3.01IEE1213 pKa = 4.57TATGSLTVTQNIATSDD1229 pKa = 3.5TGSAAIVLDD1238 pKa = 4.18AGNATAAGIATGGDD1252 pKa = 3.03IVLGGTPSPSISVGSGGKK1270 pKa = 9.6AVLYY1274 pKa = 8.73TGSVSGSTGIGALVGSGNSRR1294 pKa = 11.84YY1295 pKa = 9.97DD1296 pKa = 3.29SSKK1299 pKa = 7.27TTPGFTVALGSSGMFAVYY1317 pKa = 10.12RR1318 pKa = 11.84EE1319 pKa = 3.97QPILGYY1325 pKa = 8.24TAGSATITYY1334 pKa = 8.95GGSVPMSSLTPTYY1347 pKa = 10.53TSGLVNGDD1355 pKa = 3.45ALAQVVSSVPSASYY1369 pKa = 10.15TSSLSSGGYY1378 pKa = 8.96QNAGIYY1384 pKa = 9.92AVSASGGVTGFGYY1397 pKa = 10.58GFQYY1401 pKa = 10.62TGGTLTVNAKK1411 pKa = 10.32ALTITASAQSTTYY1424 pKa = 9.15GTALALGTSQFTPSGLVNGDD1444 pKa = 3.23SVAGVTLTQGGNATVPVTQAAGTYY1468 pKa = 8.31TGSTNGILASGATGTGLSNYY1488 pKa = 7.82TISYY1492 pKa = 9.98VPGTLTIDD1500 pKa = 3.82PKK1502 pKa = 11.25ALTVSGEE1509 pKa = 3.71AALNKK1514 pKa = 9.88VYY1516 pKa = 10.74DD1517 pKa = 4.1GSTTATLTGGTLPGLINGDD1536 pKa = 3.91SVTLTQAGTFASKK1549 pKa = 10.9NVGTNITVTASDD1561 pKa = 4.52SISGPSAGNYY1571 pKa = 8.76TLMQPTGLTANITQLASVTWIGGASGNWFDD1601 pKa = 3.78PANWAGGAVPDD1612 pKa = 4.64LSNVANVVIPQGVTVTFDD1630 pKa = 3.19SANLVAPAQGGPVNLASVGSAGNLTMAAGTLNVSSGVRR1668 pKa = 11.84LNTLAQTGGTVDD1680 pKa = 3.14SNGPVAVDD1688 pKa = 3.39VLTQSGGTLGGAGSVTASSLMQSGGSIVVGGNVTAGTLTQTAGSIDD1734 pKa = 3.28NGGNVGAVALAQSGGQIANRR1754 pKa = 11.84GGLDD1758 pKa = 3.26VTQSFSQSAPGSIAVGGSIAITQAAGNLSFVSLAGNDD1795 pKa = 2.91IDD1797 pKa = 5.75LGANSGAVNLGALDD1811 pKa = 3.77AAGSLTVNASGNISQQPGSVIRR1833 pKa = 11.84VATNVAMNSTAGAVTVSTAGNSFGSGLDD1861 pKa = 3.45SASPAQAAQSSVAADD1876 pKa = 3.43VEE1878 pKa = 4.73SGIEE1882 pKa = 3.95FTPEE1886 pKa = 3.22AGNLVEE1892 pKa = 4.78PALHH1896 pKa = 6.2TEE1898 pKa = 4.77PGQGMGTAGGSSEE1911 pKa = 3.95PAVAQEE1917 pKa = 4.22SGGAAVQPLAGIRR1930 pKa = 11.84LTVLDD1935 pKa = 3.96GGVRR1939 pKa = 11.84LPEE1942 pKa = 3.95LRR1944 pKa = 4.39

Molecular weight: 188.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1H2R9|A0A0A1H2R9_9BURK Bifunctional purine biosynthesis protein PurH OS=Burkholderiales bacterium GJ-E10 OX=1469502 GN=purH PE=3 SV=1
MM1 pKa = 8.06DD2 pKa = 5.77GIGWAWDD9 pKa = 3.6RR10 pKa = 11.84DD11 pKa = 4.2DD12 pKa = 4.75TDD14 pKa = 3.16TPYY17 pKa = 11.38AGLQQLAEE25 pKa = 4.25PPGEE29 pKa = 3.89YY30 pKa = 10.18SIFLATLHH38 pKa = 6.48PAGLSSSALIPINDD52 pKa = 3.54HH53 pKa = 5.92SAGVDD58 pKa = 3.79LSCHH62 pKa = 5.1IMARR66 pKa = 11.84SRR68 pKa = 11.84WGIVSADD75 pKa = 3.34PAGPLRR81 pKa = 11.84EE82 pKa = 3.89PAGRR86 pKa = 11.84VDD88 pKa = 3.66TGKK91 pKa = 10.11HH92 pKa = 4.95GMPIHH97 pKa = 6.16NAVIAATFTEE107 pKa = 4.36IADD110 pKa = 3.97LLEE113 pKa = 5.43IEE115 pKa = 4.37QANPFRR121 pKa = 11.84IGAYY125 pKa = 9.63RR126 pKa = 11.84KK127 pKa = 8.73AARR130 pKa = 11.84IARR133 pKa = 11.84ARR135 pKa = 11.84GLKK138 pKa = 9.96LNEE141 pKa = 3.79YY142 pKa = 9.9GVFRR146 pKa = 11.84GTEE149 pKa = 4.21RR150 pKa = 11.84IAGAAARR157 pKa = 11.84GGDD160 pKa = 3.41AVNWEE165 pKa = 4.55TFPHH169 pKa = 6.2EE170 pKa = 3.78ADD172 pKa = 2.91IGVRR176 pKa = 11.84GTGATMAEE184 pKa = 4.36AFAGAATALTAVVCDD199 pKa = 4.2PAAVGAAEE207 pKa = 4.3TTRR210 pKa = 11.84IEE212 pKa = 4.35CTAPDD217 pKa = 4.79DD218 pKa = 4.65EE219 pKa = 6.03LLLLDD224 pKa = 4.3WLNALIYY231 pKa = 10.68EE232 pKa = 4.33MATRR236 pKa = 11.84RR237 pKa = 11.84LLFSRR242 pKa = 11.84FAVRR246 pKa = 11.84ITGHH250 pKa = 6.46RR251 pKa = 11.84LRR253 pKa = 11.84ATAWGSTFPAACARR267 pKa = 11.84CIPASSAPTSSAKK280 pKa = 9.55RR281 pKa = 11.84RR282 pKa = 11.84RR283 pKa = 11.84WPISCLRR290 pKa = 11.84AFPPGSAAPAACVSMRR306 pKa = 11.84TEE308 pKa = 3.6WMRR311 pKa = 11.84CSKK314 pKa = 10.48AVRR317 pKa = 11.84AGPSSKK323 pKa = 10.72ASAVRR328 pKa = 11.84PTSRR332 pKa = 11.84ASRR335 pKa = 11.84STAASQAPIPARR347 pKa = 11.84FPSRR351 pKa = 11.84RR352 pKa = 11.84RR353 pKa = 11.84SASATRR359 pKa = 11.84WEE361 pKa = 4.29RR362 pKa = 11.84SVPATTISKK371 pKa = 10.14SRR373 pKa = 11.84SSKK376 pKa = 10.3PSSTPPRR383 pKa = 11.84RR384 pKa = 11.84APMAWRR390 pKa = 11.84RR391 pKa = 11.84TTWRR395 pKa = 3.45

Molecular weight: 42.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2955

0

2955

952706

40

7564

322.4

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.445 ± 0.075

0.966 ± 0.017

5.569 ± 0.033

5.411 ± 0.052

3.447 ± 0.033

8.412 ± 0.061

2.282 ± 0.024

4.711 ± 0.028

2.506 ± 0.037

10.096 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.227 ± 0.022

2.424 ± 0.036

5.457 ± 0.038

3.496 ± 0.028

8.263 ± 0.07

5.144 ± 0.064

5.023 ± 0.05

7.555 ± 0.044

1.374 ± 0.021

2.192 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski