Murid herpesvirus 4 (MuHV-4) (Murine gammaherpesvirus 68)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q98336|Q98336_MHV68 Envelope glycoprotein B OS=Murid herpesvirus 4 OX=33708 GN=8 PE=3 SV=1
MM1 pKa = 7.58CGVKK5 pKa = 10.29SLAKK9 pKa = 10.34CFLLFQIISFLGNHH23 pKa = 5.98NLVWVPGAALGAAEE37 pKa = 4.32TVEE40 pKa = 5.03GITSRR45 pKa = 11.84EE46 pKa = 3.74MEE48 pKa = 4.23INATKK53 pKa = 10.49APSSGATFSLLVTLSNNNPTTIMRR77 pKa = 11.84PPVAQNGEE85 pKa = 4.54SVHH88 pKa = 6.6KK89 pKa = 10.11DD90 pKa = 2.98ARR92 pKa = 11.84SASASDD98 pKa = 3.6PTTSEE103 pKa = 3.72PTSPGEE109 pKa = 4.34EE110 pKa = 4.11PTEE113 pKa = 4.42ADD115 pKa = 3.46PKK117 pKa = 9.53AAPSAGHH124 pKa = 5.74VGEE127 pKa = 4.99TEE129 pKa = 4.24PEE131 pKa = 4.31SPTPLPATPKK141 pKa = 10.28PSSQEE146 pKa = 4.07DD147 pKa = 4.33NPTMTPPTAEE157 pKa = 4.61PPTSNADD164 pKa = 3.03VSTEE168 pKa = 3.93HH169 pKa = 7.51VDD171 pKa = 3.35EE172 pKa = 5.04TEE174 pKa = 4.32PEE176 pKa = 4.4SPTFLPPTPEE186 pKa = 4.94PDD188 pKa = 3.38TPTTPEE194 pKa = 4.0TTTPSQNQEE203 pKa = 4.18DD204 pKa = 5.16EE205 pKa = 4.49PTLTTSSSDD214 pKa = 3.52APADD218 pKa = 3.79TSDD221 pKa = 3.37TSPPKK226 pKa = 10.48QEE228 pKa = 4.89DD229 pKa = 4.1DD230 pKa = 3.6PVKK233 pKa = 9.38PTEE236 pKa = 4.54SKK238 pKa = 10.39PQAEE242 pKa = 4.78PKK244 pKa = 10.41DD245 pKa = 3.93NSPSDD250 pKa = 3.81VPEE253 pKa = 4.39TADD256 pKa = 4.38SPTDD260 pKa = 3.51PASPTVEE267 pKa = 4.05LTPPTEE273 pKa = 4.31PPTPEE278 pKa = 4.09TVSPADD284 pKa = 3.54SPVPQPTAPAEE295 pKa = 4.03PSKK298 pKa = 10.77PEE300 pKa = 3.75PTPPVDD306 pKa = 4.38PPATEE311 pKa = 4.58PNTPADD317 pKa = 3.83PSTPEE322 pKa = 4.0STPPTDD328 pKa = 3.97PPAPQPTPPAEE339 pKa = 4.28PSNPEE344 pKa = 3.56PTPPVDD350 pKa = 4.83PPATPPNIPADD361 pKa = 3.79PSTPEE366 pKa = 3.94STPPADD372 pKa = 4.08PPAPQPTPPAEE383 pKa = 4.4PSTPEE388 pKa = 3.56PSTPAKK394 pKa = 10.49APAPEE399 pKa = 4.31PTPPPSGPSMTPEE412 pKa = 3.78ATPPSTAGPGAEE424 pKa = 4.41TEE426 pKa = 4.45TPDD429 pKa = 5.12GDD431 pKa = 4.23TTTQPASPQTTAPMHH446 pKa = 5.9PVPDD450 pKa = 3.58ISTLLWIRR458 pKa = 11.84PTIAIILIFLLMTIFHH474 pKa = 7.05IMYY477 pKa = 8.83CVCLHH482 pKa = 6.12EE483 pKa = 4.44

Molecular weight: 49.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O41940|O41940_MHV68 Thymidine kinase OS=Murid herpesvirus 4 OX=33708 GN=GAMMAHV.ORF21 PE=3 SV=1
MM1 pKa = 7.5NEE3 pKa = 4.02LGAKK7 pKa = 9.27QLLNKK12 pKa = 9.92LPKK15 pKa = 9.66RR16 pKa = 11.84RR17 pKa = 11.84ARR19 pKa = 11.84AGKK22 pKa = 8.89LAHH25 pKa = 5.4VRR27 pKa = 11.84CYY29 pKa = 10.57RR30 pKa = 11.84AMQGASNILQLLEE43 pKa = 4.08SLKK46 pKa = 10.16IAHH49 pKa = 6.52QITHH53 pKa = 6.65PVGSRR58 pKa = 11.84LFFEE62 pKa = 4.5VTLGRR67 pKa = 11.84RR68 pKa = 11.84VVDD71 pKa = 4.63AIIVVFSEE79 pKa = 4.29SSPHH83 pKa = 4.79IHH85 pKa = 6.93CFLIEE90 pKa = 4.49LKK92 pKa = 9.19TCKK95 pKa = 10.01INFFNQHH102 pKa = 4.12STTRR106 pKa = 11.84EE107 pKa = 3.79AQKK110 pKa = 10.96VEE112 pKa = 3.84GSNQLRR118 pKa = 11.84DD119 pKa = 3.54SAKK122 pKa = 10.48ALAVLAPVGTDD133 pKa = 3.17PCRR136 pKa = 11.84VTAHH140 pKa = 7.4LIFKK144 pKa = 8.06SQRR147 pKa = 11.84GLNTLKK153 pKa = 10.68SYY155 pKa = 8.91TLNWMTHH162 pKa = 4.38TVNTQKK168 pKa = 10.73VALLNFLGLRR178 pKa = 11.84ADD180 pKa = 3.84NEE182 pKa = 3.89LRR184 pKa = 11.84ACLTRR189 pKa = 11.84GLPPANSPGSRR200 pKa = 11.84RR201 pKa = 11.84HH202 pKa = 5.56HH203 pKa = 6.06VCLPEE208 pKa = 4.44PKK210 pKa = 9.51PQKK213 pKa = 9.36HH214 pKa = 5.53LKK216 pKa = 8.59NRR218 pKa = 11.84RR219 pKa = 11.84GGAHH223 pKa = 7.14RR224 pKa = 11.84NQKK227 pKa = 9.72ARR229 pKa = 11.84RR230 pKa = 11.84QGVGPKK236 pKa = 9.95VSNDD240 pKa = 2.91KK241 pKa = 9.89TRR243 pKa = 11.84NAPTHH248 pKa = 5.79AEE250 pKa = 3.98GRR252 pKa = 11.84AGG254 pKa = 3.63

Molecular weight: 28.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

35020

75

2457

479.7

53.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.982 ± 0.217

2.613 ± 0.226

4.84 ± 0.131

5.197 ± 0.146

4.517 ± 0.193

5.086 ± 0.209

2.841 ± 0.102

6.017 ± 0.189

5.157 ± 0.267

10.528 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.113

4.417 ± 0.144

6.205 ± 0.401

3.798 ± 0.147

4.209 ± 0.187

8.198 ± 0.202

7.102 ± 0.24

6.288 ± 0.185

1.059 ± 0.068

3.458 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski