[Actinobacillus] rossii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria;

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2555 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A380U565|A0A380U565_9PAST Domain of uncharacterized function (DUF477) OS=[Actinobacillus] rossii OX=123820 GN=NCTC10801_02544 PE=4 SV=1
MM1 pKa = 7.63GSCTYY6 pKa = 10.88NSDD9 pKa = 3.23GTISFAGYY17 pKa = 10.57GDD19 pKa = 3.87YY20 pKa = 10.97KK21 pKa = 10.51IDD23 pKa = 4.29DD24 pKa = 4.01VFDD27 pKa = 4.33PEE29 pKa = 4.13QNAEE33 pKa = 3.98AKK35 pKa = 10.3ALYY38 pKa = 10.11GPTGGFQSGIGGFYY52 pKa = 10.59SGGTAIFNYY61 pKa = 9.6RR62 pKa = 11.84QGGFWDD68 pKa = 3.64NLVEE72 pKa = 4.49SFAGTHH78 pKa = 6.6DD79 pKa = 4.37LLGGQIWGWYY89 pKa = 8.32DD90 pKa = 3.49DD91 pKa = 4.41KK92 pKa = 11.9GNTSSKK98 pKa = 10.08TSFQQGASDD107 pKa = 3.73VTTVVALPVSMPFAISDD124 pKa = 3.93LVSSDD129 pKa = 3.33FVEE132 pKa = 4.48VLFKK136 pKa = 11.26LGGNN140 pKa = 3.79

Molecular weight: 14.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A380U6G5|A0A380U6G5_9PAST 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB OS=[Actinobacillus] rossii OX=123820 GN=rlmB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.33NGRR28 pKa = 11.84QVLTRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.12GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2555

0

2555

771604

29

3720

302.0

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.443 ± 0.062

1.051 ± 0.018

5.159 ± 0.04

6.33 ± 0.043

4.325 ± 0.044

6.75 ± 0.054

2.06 ± 0.022

6.951 ± 0.047

6.278 ± 0.041

10.194 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.027

4.951 ± 0.05

3.623 ± 0.031

4.653 ± 0.04

4.392 ± 0.043

5.784 ± 0.04

5.541 ± 0.052

6.714 ± 0.042

1.17 ± 0.016

3.177 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski