Bacillus urumqiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalicoccus

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3136 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P6MIU2|A0A2P6MIU2_9BACI Uncharacterized protein OS=Bacillus urumqiensis OX=1548213 GN=C6I21_05130 PE=4 SV=1
MM1 pKa = 6.99VRR3 pKa = 11.84YY4 pKa = 7.51RR5 pKa = 11.84TQIMAAALSGAVLFPAAAGASHH27 pKa = 7.07SFPDD31 pKa = 4.06VGDD34 pKa = 3.63AHH36 pKa = 6.91WAHH39 pKa = 7.19DD40 pKa = 3.75VTGYY44 pKa = 8.8LTDD47 pKa = 4.29LNILGGYY54 pKa = 8.83PDD56 pKa = 5.81GSFQPNRR63 pKa = 11.84EE64 pKa = 3.91LTRR67 pKa = 11.84AEE69 pKa = 3.91TAVILTRR76 pKa = 11.84ALDD79 pKa = 4.02LDD81 pKa = 4.25LSSDD85 pKa = 3.46NDD87 pKa = 3.58FTDD90 pKa = 3.38VSEE93 pKa = 4.75SSFAASSIAAVADD106 pKa = 3.73AGIMNGNDD114 pKa = 2.9GKK116 pKa = 10.77FFPGRR121 pKa = 11.84SLTRR125 pKa = 11.84AEE127 pKa = 3.96MAAVLTRR134 pKa = 11.84AFNPDD139 pKa = 3.69AIQTEE144 pKa = 4.53TRR146 pKa = 11.84NFPDD150 pKa = 3.61LVDD153 pKa = 4.19GAWYY157 pKa = 9.69SYY159 pKa = 9.36PVEE162 pKa = 4.82RR163 pKa = 11.84ISRR166 pKa = 11.84AGMTEE171 pKa = 4.76GYY173 pKa = 9.56PDD175 pKa = 3.74GTFQPNRR182 pKa = 11.84DD183 pKa = 3.21LTRR186 pKa = 11.84AEE188 pKa = 3.83FATFIGRR195 pKa = 11.84AMDD198 pKa = 3.91PEE200 pKa = 4.46MFSDD204 pKa = 5.48AYY206 pKa = 10.43AAKK209 pKa = 10.04QKK211 pKa = 10.93ADD213 pKa = 3.71EE214 pKa = 4.39VVDD217 pKa = 4.12ALAAADD223 pKa = 3.47MGEE226 pKa = 4.06FSSYY230 pKa = 10.89VYY232 pKa = 10.4TDD234 pKa = 3.19GVRR237 pKa = 11.84FTPYY241 pKa = 10.77GYY243 pKa = 8.9VTDD246 pKa = 5.05DD247 pKa = 4.0DD248 pKa = 4.61VLFGAGEE255 pKa = 4.75IEE257 pKa = 4.39DD258 pKa = 3.7AWNSGEE264 pKa = 3.9DD265 pKa = 4.51FYY267 pKa = 11.28WGDD270 pKa = 3.61YY271 pKa = 11.17DD272 pKa = 4.38GTGDD276 pKa = 5.95DD277 pKa = 4.69IIQSFSEE284 pKa = 4.67YY285 pKa = 9.86YY286 pKa = 10.77DD287 pKa = 3.28EE288 pKa = 6.09FIYY291 pKa = 11.06DD292 pKa = 3.61RR293 pKa = 11.84DD294 pKa = 3.95YY295 pKa = 12.04ASAPQTAYY303 pKa = 10.17NQRR306 pKa = 11.84IGEE309 pKa = 4.28GNSLNNIDD317 pKa = 4.58EE318 pKa = 4.52YY319 pKa = 11.67YY320 pKa = 9.41PDD322 pKa = 3.73GVFVEE327 pKa = 4.52YY328 pKa = 9.78HH329 pKa = 5.83YY330 pKa = 10.96PGSDD334 pKa = 2.83EE335 pKa = 4.1FAGMDD340 pKa = 3.37WSSLRR345 pKa = 11.84IVMVEE350 pKa = 4.72SDD352 pKa = 4.05GEE354 pKa = 4.35WYY356 pKa = 10.29VVGVIHH362 pKa = 7.5DD363 pKa = 3.58EE364 pKa = 3.97WTII367 pKa = 3.33

Molecular weight: 40.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P6MDL9|A0A2P6MDL9_9BACI Uncharacterized protein OS=Bacillus urumqiensis OX=1548213 GN=C6I21_14840 PE=4 SV=1
MM1 pKa = 7.69GKK3 pKa = 7.98PTFNPNNRR11 pKa = 11.84KK12 pKa = 9.57RR13 pKa = 11.84KK14 pKa = 7.65MNHH17 pKa = 4.64GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37NGRR29 pKa = 11.84KK30 pKa = 9.17VLANRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.69GRR40 pKa = 11.84KK41 pKa = 8.69VLSAA45 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3136

0

3136

944850

31

2046

301.3

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.758 ± 0.048

0.656 ± 0.011

5.515 ± 0.045

8.57 ± 0.057

4.174 ± 0.032

7.459 ± 0.041

2.232 ± 0.022

5.871 ± 0.041

4.735 ± 0.045

9.534 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.974 ± 0.021

3.178 ± 0.03

4.088 ± 0.029

3.833 ± 0.03

5.38 ± 0.036

5.954 ± 0.033

5.633 ± 0.027

7.196 ± 0.036

1.127 ± 0.018

3.132 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski