Bacillus sp. (strain OxB-1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3544 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8JE01|A0A0A8JE01_BACSX Hypothetical dehydrogenase OS=Bacillus sp. (strain OxB-1) OX=98228 GN=OXB_0130 PE=4 SV=1
MM1 pKa = 7.58ASSEE5 pKa = 4.15AAALAAEE12 pKa = 4.59EE13 pKa = 4.4GLADD17 pKa = 3.67SAEE20 pKa = 4.12VVPAVEE26 pKa = 4.72AEE28 pKa = 3.88AAEE31 pKa = 4.61AGDD34 pKa = 3.86PRR36 pKa = 11.84NGMRR40 pKa = 11.84ACHH43 pKa = 6.39SFFDD47 pKa = 4.21YY48 pKa = 11.03TKK50 pKa = 8.22GTKK53 pKa = 9.67PNEE56 pKa = 4.13VYY58 pKa = 10.7PMQLTFYY65 pKa = 9.52TRR67 pKa = 11.84AGCPLCEE74 pKa = 4.05EE75 pKa = 4.81AKK77 pKa = 10.76RR78 pKa = 11.84MLQLAQDD85 pKa = 4.85DD86 pKa = 5.19YY87 pKa = 11.05PFTWTEE93 pKa = 3.37VDD95 pKa = 3.96IEE97 pKa = 4.18QDD99 pKa = 3.33DD100 pKa = 4.2ATHH103 pKa = 6.08EE104 pKa = 4.55KK105 pKa = 9.82YY106 pKa = 10.3MLMIPVIEE114 pKa = 4.57IDD116 pKa = 3.85GQVALYY122 pKa = 10.68GSIGYY127 pKa = 8.53MDD129 pKa = 5.03IIGLFEE135 pKa = 3.86

Molecular weight: 14.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8JGI1|A0A0A8JGI1_BACSX ATP binding cassette transporter permease protein OS=Bacillus sp. (strain OxB-1) OX=98228 GN=OXB_1263 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.67SGRR28 pKa = 11.84RR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3544

0

3544

1011903

39

2070

285.5

31.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.988 ± 0.046

0.636 ± 0.011

5.318 ± 0.031

7.832 ± 0.051

4.402 ± 0.03

7.259 ± 0.038

2.071 ± 0.021

7.256 ± 0.039

5.965 ± 0.036

9.748 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.93 ± 0.019

3.683 ± 0.024

3.872 ± 0.026

3.632 ± 0.024

4.704 ± 0.035

5.756 ± 0.03

5.532 ± 0.031

7.246 ± 0.031

0.982 ± 0.014

3.188 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski