Hubei tombus-like virus 39

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGI9|A0A1L3KGI9_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 39 OX=1923287 PE=4 SV=1
MM1 pKa = 7.72TILKK5 pKa = 10.57GKK7 pKa = 9.16VCSINSNEE15 pKa = 4.42AVTCQLFHH23 pKa = 6.75AAAFRR28 pKa = 11.84RR29 pKa = 11.84ILMSFDD35 pKa = 3.13GFQIQSKK42 pKa = 10.45LKK44 pKa = 10.57LNYY47 pKa = 9.74EE48 pKa = 3.87PSSRR52 pKa = 11.84LNAGVVGVIVTVGIKK67 pKa = 10.23CAMEE71 pKa = 4.32NPTDD75 pKa = 3.5VCQYY79 pKa = 10.46APHH82 pKa = 6.67AAEE85 pKa = 4.71GMGGQANTSHH95 pKa = 5.96SMKK98 pKa = 10.35YY99 pKa = 10.48DD100 pKa = 4.43LAPMDD105 pKa = 4.41LVLEE109 pKa = 4.5SEE111 pKa = 4.02LHH113 pKa = 5.12EE114 pKa = 4.33RR115 pKa = 11.84MVYY118 pKa = 8.47VTVAGLDD125 pKa = 4.37SEE127 pKa = 4.99DD128 pKa = 3.51HH129 pKa = 6.01WLGDD133 pKa = 3.56LTYY136 pKa = 9.48EE137 pKa = 3.9LHH139 pKa = 5.26VQEE142 pKa = 5.65RR143 pKa = 11.84YY144 pKa = 9.12PSPIALFAVNNSIRR158 pKa = 11.84MANCKK163 pKa = 9.95RR164 pKa = 11.84SVSGTTYY171 pKa = 10.59TNVLATTTTIHH182 pKa = 6.56SSNHH186 pKa = 6.02DD187 pKa = 3.34SSGFSTGVGSSITEE201 pKa = 4.14LEE203 pKa = 3.89VDD205 pKa = 4.51SIVTNNIQTLWFNVRR220 pKa = 11.84TCGYY224 pKa = 7.0NTLYY228 pKa = 10.84FIGIGSLGLNFVLSNFNDD246 pKa = 3.61TDD248 pKa = 3.7GVEE251 pKa = 4.54VIDD254 pKa = 5.1SGEE257 pKa = 3.96IDD259 pKa = 4.98DD260 pKa = 5.91DD261 pKa = 5.32PNTSWYY267 pKa = 7.91ITFYY271 pKa = 10.68CRR273 pKa = 11.84KK274 pKa = 6.67TTDD277 pKa = 3.04IPLLSVDD284 pKa = 3.33QTTDD288 pKa = 2.96GFLVEE293 pKa = 4.66YY294 pKa = 9.55PFLLSTVTYY303 pKa = 9.76GSPVEE308 pKa = 4.77HH309 pKa = 6.2NTSVAKK315 pKa = 10.3VRR317 pKa = 11.84NLNKK321 pKa = 9.9HH322 pKa = 5.06QLAMLPPTCILPATTSHH339 pKa = 7.1SDD341 pKa = 3.08TSDD344 pKa = 2.95NRR346 pKa = 11.84PQHH349 pKa = 4.39QAFRR353 pKa = 11.84HH354 pKa = 4.89TEE356 pKa = 3.88ATKK359 pKa = 8.52TTYY362 pKa = 10.96SKK364 pKa = 11.56

Molecular weight: 40.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGP9|A0A1L3KGP9_9VIRU Chropara_Vmeth domain-containing protein OS=Hubei tombus-like virus 39 OX=1923287 PE=4 SV=1
MM1 pKa = 7.74EE2 pKa = 5.01FPTWVARR9 pKa = 11.84YY10 pKa = 7.18TGGRR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 9.4QLQRR20 pKa = 11.84EE21 pKa = 4.1FDD23 pKa = 3.37TGHH26 pKa = 6.44WKK28 pKa = 10.46VADD31 pKa = 3.91EE32 pKa = 4.35AASGVKK38 pKa = 10.26SFIKK42 pKa = 10.27IEE44 pKa = 4.23AGKK47 pKa = 8.27PTDD50 pKa = 3.91PRR52 pKa = 11.84NISPRR57 pKa = 11.84PDD59 pKa = 3.08EE60 pKa = 4.02YY61 pKa = 11.14LVRR64 pKa = 11.84LGPIIAGIEE73 pKa = 3.92RR74 pKa = 11.84DD75 pKa = 4.9FKK77 pKa = 11.39CPWAVKK83 pKa = 9.75HH84 pKa = 6.03LSVSQRR90 pKa = 11.84DD91 pKa = 3.77VHH93 pKa = 6.07VAEE96 pKa = 4.43YY97 pKa = 10.86LGLTPVSTVIDD108 pKa = 3.48IDD110 pKa = 3.27QSRR113 pKa = 11.84FDD115 pKa = 3.46RR116 pKa = 11.84HH117 pKa = 6.09ISRR120 pKa = 11.84FWLYY124 pKa = 11.38VEE126 pKa = 4.73TEE128 pKa = 4.08LLSYY132 pKa = 9.39MCHH135 pKa = 6.54PDD137 pKa = 3.42DD138 pKa = 4.42RR139 pKa = 11.84QEE141 pKa = 5.35LRR143 pKa = 11.84ALLNKK148 pKa = 10.27QIVTRR153 pKa = 11.84GFHH156 pKa = 5.04TQGIRR161 pKa = 11.84YY162 pKa = 6.94STVGGRR168 pKa = 11.84CSGDD172 pKa = 3.13ANTSIGNTILNCLITQLAFCSLGYY196 pKa = 9.5TDD198 pKa = 5.91FKK200 pKa = 11.17SVHH203 pKa = 6.91DD204 pKa = 4.81GDD206 pKa = 5.45DD207 pKa = 3.71GLITIYY213 pKa = 10.69GPIMRR218 pKa = 11.84VEE220 pKa = 4.15NNHH223 pKa = 6.13ALEE226 pKa = 4.32SLVEE230 pKa = 4.31DD231 pKa = 3.42LTEE234 pKa = 3.94AMRR237 pKa = 11.84VLGFDD242 pKa = 3.58AKK244 pKa = 10.6IKK246 pKa = 9.31IHH248 pKa = 6.78HH249 pKa = 7.54DD250 pKa = 2.4IHH252 pKa = 6.16TATFDD257 pKa = 3.4GRR259 pKa = 11.84MLYY262 pKa = 10.2FDD264 pKa = 3.98RR265 pKa = 11.84GEE267 pKa = 4.33VKK269 pKa = 10.87SMADD273 pKa = 3.43PLRR276 pKa = 11.84TIVKK280 pKa = 8.34MHH282 pKa = 5.82MTAHH286 pKa = 6.52PTQEE290 pKa = 3.97KK291 pKa = 10.41DD292 pKa = 2.94WTVKK296 pKa = 10.24LRR298 pKa = 11.84NSLLLAKK305 pKa = 9.76ALSYY309 pKa = 10.57AASDD313 pKa = 4.0LDD315 pKa = 3.97TPIIGAWCQFVIRR328 pKa = 11.84NLTHH332 pKa = 6.31NVSHH336 pKa = 7.35PLSKK340 pKa = 10.69SNKK343 pKa = 8.75KK344 pKa = 9.01MLTPRR349 pKa = 11.84YY350 pKa = 8.22PKK352 pKa = 9.93EE353 pKa = 3.6IKK355 pKa = 10.05RR356 pKa = 11.84RR357 pKa = 11.84MDD359 pKa = 4.06LAPIPKK365 pKa = 9.82GYY367 pKa = 10.48LWALRR372 pKa = 11.84KK373 pKa = 10.03RR374 pKa = 11.84NTTIEE379 pKa = 3.51ARR381 pKa = 11.84AAFFVNSNIGTIEE394 pKa = 3.94QEE396 pKa = 4.22MMEE399 pKa = 4.08VWLDD403 pKa = 3.2GSNNIDD409 pKa = 4.39HH410 pKa = 7.31IPPKK414 pKa = 9.88LTIAPRR420 pKa = 11.84EE421 pKa = 3.67FDD423 pKa = 5.43LNDD426 pKa = 3.5LYY428 pKa = 11.77YY429 pKa = 10.62FFSSTHH435 pKa = 6.3EE436 pKa = 3.97YY437 pKa = 8.22HH438 pKa = 6.51TLL440 pKa = 3.35

Molecular weight: 50.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2038

364

1234

679.3

75.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.458 ± 0.724

1.766 ± 0.152

5.545 ± 0.424

5.643 ± 0.365

3.729 ± 0.138

6.771 ± 0.488

2.502 ± 0.713

5.005 ± 0.788

4.269 ± 0.405

8.096 ± 0.397

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.846 ± 0.132

3.729 ± 0.796

5.348 ± 0.55

2.846 ± 0.11

6.084 ± 0.788

8.342 ± 0.768

7.066 ± 1.013

8.096 ± 0.903

1.276 ± 0.186

3.582 ± 0.081
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski