Caloramator australicus RC3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Caloramator; Caloramator australicus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7K658|I7K658_9CLOT Uncharacterized protein OS=Caloramator australicus RC3 OX=857293 GN=CAAU_0960 PE=4 SV=1
MM1 pKa = 6.43QTNEE5 pKa = 4.1YY6 pKa = 9.66VDD8 pKa = 3.92VYY10 pKa = 10.08LTVSGDD16 pKa = 2.84TWDD19 pKa = 3.78IISYY23 pKa = 10.04KK24 pKa = 10.73VYY26 pKa = 10.86GDD28 pKa = 3.45EE29 pKa = 4.51NYY31 pKa = 10.46VKK33 pKa = 10.59EE34 pKa = 4.31LMEE37 pKa = 5.05ANPSYY42 pKa = 10.52MSITIFPSGIDD53 pKa = 3.35IIIPDD58 pKa = 4.21IEE60 pKa = 4.38IKK62 pKa = 9.79DD63 pKa = 3.79TSAQLPWKK71 pKa = 10.39EE72 pKa = 3.93EE73 pKa = 3.83

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7KVN2|I7KVN2_9CLOT Putative GTP-binding protien TM0445 OS=Caloramator australicus RC3 OX=857293 GN=CAAU_2020 PE=4 SV=1
MM1 pKa = 7.5LRR3 pKa = 11.84TFQPKK8 pKa = 9.06KK9 pKa = 8.34RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.78RR21 pKa = 11.84MKK23 pKa = 8.53TRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 7.43VLKK32 pKa = 9.55RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84MKK37 pKa = 10.3GRR39 pKa = 11.84KK40 pKa = 9.06RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2679

0

2679

766429

37

2840

286.1

32.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.855 ± 0.052

0.97 ± 0.019

5.561 ± 0.043

7.631 ± 0.062

4.753 ± 0.039

6.348 ± 0.049

1.337 ± 0.021

10.254 ± 0.049

9.287 ± 0.058

9.481 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.397 ± 0.019

5.709 ± 0.044

3.059 ± 0.033

2.248 ± 0.025

3.94 ± 0.035

5.395 ± 0.036

4.404 ± 0.037

6.454 ± 0.048

0.683 ± 0.016

4.232 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski