Pseudomonas sp. StFLB209

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5594 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077LJQ1|A0A077LJQ1_9PSED Imidazoleglycerol-phosphate dehydratase OS=Pseudomonas sp. StFLB209 OX=1028989 GN=hisB PE=3 SV=1
MM1 pKa = 7.39AVINGTSGADD11 pKa = 3.47TLTGTSDD18 pKa = 3.2SDD20 pKa = 4.23QINGLAGNDD29 pKa = 4.0VIHH32 pKa = 6.92SSAGADD38 pKa = 3.44KK39 pKa = 10.71IVGGVGTDD47 pKa = 3.07TVDD50 pKa = 3.33YY51 pKa = 10.2SKK53 pKa = 10.9SAEE56 pKa = 4.45GISIDD61 pKa = 3.35VRR63 pKa = 11.84AGVGLAGRR71 pKa = 11.84GGDD74 pKa = 3.51AEE76 pKa = 4.53GDD78 pKa = 3.27ILEE81 pKa = 4.47TVEE84 pKa = 4.4KK85 pKa = 10.96VVGSVFNDD93 pKa = 3.42TLAIGAIATMVTLEE107 pKa = 4.6GGAGDD112 pKa = 3.61DD113 pKa = 4.09TYY115 pKa = 11.05IIGHH119 pKa = 7.58DD120 pKa = 3.58GTPIIVEE127 pKa = 4.04QANGGNDD134 pKa = 3.49EE135 pKa = 4.11VRR137 pKa = 11.84VTVLSKK143 pKa = 11.07QNTRR147 pKa = 11.84LADD150 pKa = 3.96NIEE153 pKa = 3.88RR154 pKa = 11.84LTYY157 pKa = 10.09VGNGAFTGYY166 pKa = 10.86GNALDD171 pKa = 4.7NIITGGSGGDD181 pKa = 3.44KK182 pKa = 10.64LYY184 pKa = 11.27GGAGADD190 pKa = 3.55QFIGGAGNDD199 pKa = 3.53TVGYY203 pKa = 10.07DD204 pKa = 3.69DD205 pKa = 3.9STTGVSINLKK215 pKa = 8.88TGEE218 pKa = 4.33KK219 pKa = 10.15SGIAAGDD226 pKa = 3.76TYY228 pKa = 11.54TDD230 pKa = 3.75IEE232 pKa = 4.81VIWGSNANDD241 pKa = 3.68TFVGDD246 pKa = 3.9GSGMDD251 pKa = 3.82FNGLGGIDD259 pKa = 3.39TVDD262 pKa = 3.49YY263 pKa = 10.6SGSASAVDD271 pKa = 3.57VSVSVGNVRR280 pKa = 11.84AGIGGDD286 pKa = 3.56AEE288 pKa = 4.3GTVLNNIEE296 pKa = 4.76RR297 pKa = 11.84IVGSAFNDD305 pKa = 3.44TLAIGTISTMVTLEE319 pKa = 4.21GGAGDD324 pKa = 3.61DD325 pKa = 4.06TYY327 pKa = 11.05IIGHH331 pKa = 6.67NGTPIVVEE339 pKa = 3.96QANGGNDD346 pKa = 3.49EE347 pKa = 4.23VRR349 pKa = 11.84VTVLNQQGTRR359 pKa = 11.84LADD362 pKa = 3.86NIEE365 pKa = 3.88RR366 pKa = 11.84LTYY369 pKa = 10.09VGNGAFTGYY378 pKa = 10.86GNALDD383 pKa = 4.7NIITGGSGNNTLYY396 pKa = 11.04GGAGDD401 pKa = 4.06DD402 pKa = 4.56QIIGGAGSDD411 pKa = 3.55TLNGGEE417 pKa = 5.21GADD420 pKa = 3.71QLIGGAGYY428 pKa = 7.99DD429 pKa = 3.45TAGYY433 pKa = 10.13ADD435 pKa = 4.88SISAGVTVNLKK446 pKa = 9.43TGIHH450 pKa = 6.08SGFAAGDD457 pKa = 3.66TYY459 pKa = 11.83VDD461 pKa = 3.51IEE463 pKa = 5.03AISGSNFNDD472 pKa = 2.84VFYY475 pKa = 10.96ADD477 pKa = 4.01SSSTGWNGVVGQDD490 pKa = 2.99MVTYY494 pKa = 8.78EE495 pKa = 4.17LSEE498 pKa = 4.14TAVTVDD504 pKa = 5.12LFTGTNAGDD513 pKa = 3.91ADD515 pKa = 4.07GDD517 pKa = 4.1TYY519 pKa = 11.85ASIEE523 pKa = 4.21IIQGSTFGDD532 pKa = 3.67TLSGSRR538 pKa = 11.84LADD541 pKa = 3.43TFIGGAGADD550 pKa = 3.9TIDD553 pKa = 3.43GRR555 pKa = 11.84EE556 pKa = 3.99GHH558 pKa = 6.89DD559 pKa = 3.7SVWYY563 pKa = 7.34ITSAAGVTINLQTQSNQGGDD583 pKa = 3.33AEE585 pKa = 5.09GDD587 pKa = 3.52VLSNIEE593 pKa = 4.28RR594 pKa = 11.84LVGSHH599 pKa = 7.0FNDD602 pKa = 4.12ALTGDD607 pKa = 3.53AGVNYY612 pKa = 10.59LEE614 pKa = 5.48GGLGDD619 pKa = 5.39DD620 pKa = 4.46IIEE623 pKa = 4.48GGEE626 pKa = 3.94GGDD629 pKa = 3.76YY630 pKa = 10.77IFGGLASQIGSFTLVGQTNGPQADD654 pKa = 3.67TLYY657 pKa = 11.01GGNGNDD663 pKa = 4.37TIVTAANDD671 pKa = 3.68LGSRR675 pKa = 11.84VYY677 pKa = 11.01GEE679 pKa = 4.51AGGDD683 pKa = 3.75VITVVHH689 pKa = 6.26GMADD693 pKa = 3.36GGDD696 pKa = 3.93GNDD699 pKa = 3.2VLTGTGLDD707 pKa = 3.65FSLFGGSGSDD717 pKa = 3.15QLVLHH722 pKa = 6.39SSGWANGGEE731 pKa = 4.3GSDD734 pKa = 3.69TYY736 pKa = 9.62TVRR739 pKa = 11.84TTKK742 pKa = 10.76LVMIQDD748 pKa = 4.51DD749 pKa = 4.15GASYY753 pKa = 11.27GDD755 pKa = 4.24KK756 pKa = 10.82LVLSQITSSEE766 pKa = 4.1LLFDD770 pKa = 4.52QIGNDD775 pKa = 3.87LYY777 pKa = 11.08LHH779 pKa = 7.07RR780 pKa = 11.84YY781 pKa = 8.48SLAAGQTPQEE791 pKa = 4.18GVLLKK796 pKa = 10.69DD797 pKa = 3.39WFAGYY802 pKa = 8.27DD803 pKa = 3.81TIEE806 pKa = 4.34HH807 pKa = 5.67VQTADD812 pKa = 3.16MQLISLSGLSPTTSSLAA829 pKa = 3.44

Molecular weight: 83.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077LKU1|A0A077LKU1_9PSED Uncharacterized protein OS=Pseudomonas sp. StFLB209 OX=1028989 GN=PSCI_4059 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.98GRR39 pKa = 11.84KK40 pKa = 8.8RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5594

0

5594

1859865

39

4311

332.5

36.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.093 ± 0.043

0.963 ± 0.011

5.345 ± 0.024

5.464 ± 0.027

3.546 ± 0.02

7.893 ± 0.032

2.272 ± 0.016

4.738 ± 0.024

3.242 ± 0.025

11.803 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.219 ± 0.017

3.095 ± 0.02

4.808 ± 0.025

4.974 ± 0.024

6.573 ± 0.032

6.031 ± 0.025

4.867 ± 0.035

7.085 ± 0.029

1.464 ± 0.013

2.525 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski