Brochothrix phage BL3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7RWN3|D7RWN3_9CAUD Gp50 OS=Brochothrix phage BL3 OX=764562 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84GDD4 pKa = 3.77YY5 pKa = 10.84DD6 pKa = 3.49GSRR9 pKa = 11.84CFKK12 pKa = 10.79CDD14 pKa = 3.79DD15 pKa = 3.6EE16 pKa = 5.17CSIVDD21 pKa = 3.6GLLVCDD27 pKa = 4.43EE28 pKa = 4.9CGQTYY33 pKa = 7.42TQQEE37 pKa = 4.14WEE39 pKa = 4.04QANEE43 pKa = 4.13KK44 pKa = 10.38EE45 pKa = 3.98IEE47 pKa = 4.13AYY49 pKa = 9.59IAFCEE54 pKa = 4.09KK55 pKa = 10.72LEE57 pKa = 4.32KK58 pKa = 10.56EE59 pKa = 4.34SAEE62 pKa = 4.03

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7RWP8|D7RWP8_9CAUD Gp65 OS=Brochothrix phage BL3 OX=764562 PE=4 SV=1
MM1 pKa = 7.96AIITDD6 pKa = 3.34KK7 pKa = 9.06TTNKK11 pKa = 9.6EE12 pKa = 4.01YY13 pKa = 10.65RR14 pKa = 11.84FKK16 pKa = 10.33THH18 pKa = 7.13AEE20 pKa = 3.85ANRR23 pKa = 11.84YY24 pKa = 8.52MIDD27 pKa = 3.22NPRR30 pKa = 11.84KK31 pKa = 9.24HH32 pKa = 6.29GYY34 pKa = 8.78KK35 pKa = 9.92VHH37 pKa = 7.07LKK39 pKa = 10.05QDD41 pKa = 3.49EE42 pKa = 4.36QADD45 pKa = 4.11RR46 pKa = 11.84LYY48 pKa = 10.97LKK50 pKa = 10.5CKK52 pKa = 10.36AFGRR56 pKa = 11.84RR57 pKa = 3.35

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13264

40

1567

198.0

22.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.8 ± 0.359

0.716 ± 0.136

6.333 ± 0.217

7.396 ± 0.414

3.762 ± 0.196

6.446 ± 0.347

1.38 ± 0.184

7.057 ± 0.257

9.612 ± 0.305

7.999 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.586 ± 0.236

5.76 ± 0.271

2.661 ± 0.186

3.762 ± 0.218

3.664 ± 0.23

6.544 ± 0.355

5.964 ± 0.25

6.627 ± 0.248

1.116 ± 0.172

3.815 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski