Dacryopinax primogenitus (strain DJM 731) (Brown rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Dacrymycetes; Dacrymycetales; Dacrymycetaceae; Dacryopinax

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10233 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M5FZK5|M5FZK5_DACPD Ribonuclease III OS=Dacryopinax primogenitus (strain DJM 731) OX=1858805 GN=DACRYDRAFT_94996 PE=4 SV=1
MM1 pKa = 7.35AHH3 pKa = 5.75YY4 pKa = 10.26ASYY7 pKa = 10.24FGSVEE12 pKa = 3.78WGVGLDD18 pKa = 3.14QAFSHH23 pKa = 7.05RR24 pKa = 11.84SNEE27 pKa = 3.93QQQQQISNNNVKK39 pKa = 9.61TDD41 pKa = 3.77LAFLSDD47 pKa = 3.88SYY49 pKa = 12.02AAMRR53 pKa = 11.84SAQCGSQIITSSNTSPISLDD73 pKa = 3.57VPSLSLADD81 pKa = 3.48IMDD84 pKa = 4.18PSFLSGLDD92 pKa = 3.52STSLEE97 pKa = 4.17MTSDD101 pKa = 4.68GINPDD106 pKa = 3.64LLASFNPYY114 pKa = 10.61LSTDD118 pKa = 3.02WSSEE122 pKa = 3.82QPQTVVPSQLWRR134 pKa = 11.84SSSPTYY140 pKa = 9.86VQPSSFDD147 pKa = 3.6LASNALAAALPGNDD161 pKa = 3.74LAA163 pKa = 6.58

Molecular weight: 17.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M5FYA0|M5FYA0_DACPD Uncharacterized protein OS=Dacryopinax primogenitus (strain DJM 731) OX=1858805 GN=DACRYDRAFT_53063 PE=4 SV=1
MM1 pKa = 7.72ASLSPRR7 pKa = 11.84SARR10 pKa = 11.84SLSLSVLSPLSAPARR25 pKa = 11.84SARR28 pKa = 11.84SLSLSRR34 pKa = 11.84SQSRR38 pKa = 11.84AFSRR42 pKa = 11.84SQSLSLSLSRR52 pKa = 11.84RR53 pKa = 11.84LSLSRR58 pKa = 11.84SHH60 pKa = 6.67SLPPALPSHH69 pKa = 6.76TFPAPAPNGLPSPPSAGAQSGVRR92 pKa = 11.84PASS95 pKa = 3.24

Molecular weight: 9.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10233

0

10233

4133171

49

5003

403.9

44.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.812 ± 0.022

1.247 ± 0.011

5.262 ± 0.017

6.289 ± 0.028

3.595 ± 0.015

6.991 ± 0.025

2.472 ± 0.01

4.642 ± 0.019

4.33 ± 0.02

9.603 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.008

3.209 ± 0.013

6.729 ± 0.03

3.862 ± 0.016

6.118 ± 0.024

7.848 ± 0.026

5.922 ± 0.016

6.484 ± 0.016

1.591 ± 0.011

2.692 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski