Vibrio phage vB_VpaP_MGD2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Colwellvirinae; Kaohsiungvirus; unclassified Kaohsiungvirus

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B7HXC0|A0A6B7HXC0_9CAUD Uncharacterized protein OS=Vibrio phage vB_VpaP_MGD2 OX=2565877 GN=vBVpaPMGD2_15 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.1NWTMQDD8 pKa = 2.42ITNATNIGTIEE19 pKa = 4.31LEE21 pKa = 3.87SGNYY25 pKa = 9.32FEE27 pKa = 6.15VFQTQDD33 pKa = 2.89ALVFGNFSNTGLIQSGYY50 pKa = 7.89MQLDD54 pKa = 3.84SYY56 pKa = 11.71LSLDD60 pKa = 4.22DD61 pKa = 4.59NLQEE65 pKa = 4.57LVQEE69 pKa = 4.35LEE71 pKa = 4.2TYY73 pKa = 9.21YY74 pKa = 10.83QDD76 pKa = 3.69GKK78 pKa = 11.58GFTTDD83 pKa = 3.66LVTNDD88 pKa = 3.3RR89 pKa = 11.84MM90 pKa = 3.92

Molecular weight: 10.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B7HXA7|A0A6B7HXA7_9CAUD N-acetyltransferase domain-containing protein OS=Vibrio phage vB_VpaP_MGD2 OX=2565877 GN=vBVpaPMGD2_22 PE=4 SV=1
MM1 pKa = 7.47TPTNVVVDD9 pKa = 4.35HH10 pKa = 6.37DD11 pKa = 4.44HH12 pKa = 6.44KK13 pKa = 11.31SGVIRR18 pKa = 11.84AALPRR23 pKa = 11.84AINGLEE29 pKa = 3.97GKK31 pKa = 8.2LTNLCIRR38 pKa = 11.84WGGCTSKK45 pKa = 10.9RR46 pKa = 11.84DD47 pKa = 4.02IINMLRR53 pKa = 11.84GMADD57 pKa = 3.46YY58 pKa = 11.31LEE60 pKa = 4.38LHH62 pKa = 5.9QVPQTEE68 pKa = 4.78WIHH71 pKa = 6.23PEE73 pKa = 3.72HH74 pKa = 6.5LTPAEE79 pKa = 3.84ARR81 pKa = 11.84AKK83 pKa = 10.41RR84 pKa = 11.84NAKK87 pKa = 9.14ARR89 pKa = 11.84KK90 pKa = 9.25RR91 pKa = 11.84YY92 pKa = 9.41ALKK95 pKa = 10.79KK96 pKa = 9.56KK97 pKa = 10.69DD98 pKa = 3.37KK99 pKa = 10.92

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

13552

41

1229

282.3

31.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.228 ± 0.512

0.959 ± 0.147

6.619 ± 0.205

7.652 ± 0.405

3.852 ± 0.198

7.084 ± 0.304

1.896 ± 0.18

5.527 ± 0.225

7.121 ± 0.286

8.021 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.099 ± 0.184

4.752 ± 0.174

3.63 ± 0.161

4.117 ± 0.256

5.121 ± 0.258

5.689 ± 0.334

5.394 ± 0.342

6.198 ± 0.315

1.402 ± 0.157

3.638 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski