Rhodopseudomonas palustris (strain BisA53)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Rhodopseudomonas; Rhodopseudomonas palustris

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4851 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q07TP0|Q07TP0_RHOP5 Extracellular solute-binding protein family 3 OS=Rhodopseudomonas palustris (strain BisA53) OX=316055 GN=RPE_0737 PE=4 SV=1
MM1 pKa = 7.25SVAATTSTTAATTATTTLSSSSSSTLSSSDD31 pKa = 3.47FLTLLVAEE39 pKa = 5.08LQNQNPLDD47 pKa = 4.06PTSTTDD53 pKa = 3.57FTSQLMSYY61 pKa = 11.14ASFEE65 pKa = 4.09QQTALNSNLSSLSTSFNSLLTLNSSNYY92 pKa = 9.44IGQTVTAKK100 pKa = 10.57ADD102 pKa = 3.46SATLEE107 pKa = 4.14NGSISFGYY115 pKa = 9.95SLNSAAKK122 pKa = 9.49NVTLTVSDD130 pKa = 3.8SSGNTVWTGSGTTTAGSNSFTWDD153 pKa = 2.95GTTTNGTQLSDD164 pKa = 3.21GGSYY168 pKa = 10.53SLSVTATDD176 pKa = 3.64SSGSSVYY183 pKa = 10.79GYY185 pKa = 6.39TTVSGTVTGVDD196 pKa = 3.5SSSGTTMLKK205 pKa = 10.08IGSTSLAASSVVGVTSS221 pKa = 3.57

Molecular weight: 22.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q07U09|Q07U09_RHOP5 ATP-grasp domain-containing protein OS=Rhodopseudomonas palustris (strain BisA53) OX=316055 GN=RPE_0617 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATVGGRR28 pKa = 11.84KK29 pKa = 9.08VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4851

0

4851

1562097

31

4651

322.0

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.84 ± 0.049

0.861 ± 0.011

5.69 ± 0.031

5.305 ± 0.041

3.634 ± 0.026

8.247 ± 0.055

1.988 ± 0.018

5.296 ± 0.023

3.505 ± 0.034

10.092 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.02

2.673 ± 0.027

5.27 ± 0.035

3.361 ± 0.021

7.107 ± 0.045

5.723 ± 0.04

5.311 ± 0.042

7.352 ± 0.026

1.265 ± 0.015

2.155 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski