Buchnera aphidicola subsp. Schlechtendalia chinensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Buchnera; Buchnera aphidicola

Average proteome isoelectric point is 8.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 499 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172WDD3|A0A172WDD3_BUCSC NAD kinase OS=Buchnera aphidicola subsp. Schlechtendalia chinensis OX=118110 GN=ppnK PE=3 SV=1
MM1 pKa = 7.42NNDD4 pKa = 3.2EE5 pKa = 4.85KK6 pKa = 11.42KK7 pKa = 10.82LIEE10 pKa = 4.5DD11 pKa = 4.23LFSRR15 pKa = 11.84LHH17 pKa = 4.96KK18 pKa = 9.83TEE20 pKa = 4.02IKK22 pKa = 10.75SGDD25 pKa = 3.58RR26 pKa = 11.84DD27 pKa = 3.81NIAEE31 pKa = 4.08NLIKK35 pKa = 10.35EE36 pKa = 4.44LLEE39 pKa = 4.56KK40 pKa = 10.91YY41 pKa = 10.25PNTPYY46 pKa = 11.6YY47 pKa = 9.4MAQTLLIQEE56 pKa = 4.31TAIKK60 pKa = 10.06KK61 pKa = 10.16LNEE64 pKa = 4.09KK65 pKa = 10.42LSSLEE70 pKa = 3.73NDD72 pKa = 3.64KK73 pKa = 11.35LKK75 pKa = 11.03NDD77 pKa = 3.84NNSKK81 pKa = 8.72GTSGSFLSNLFGSKK95 pKa = 9.92KK96 pKa = 9.5PKK98 pKa = 10.15NSSEE102 pKa = 4.05PALGTSCNFPKK113 pKa = 10.46DD114 pKa = 4.02SNVGSPSLYY123 pKa = 9.34TRR125 pKa = 11.84SNTMSPTGVMNNNSSSFLGGALQTAAGVAGGVVMANALMNLFQNKK170 pKa = 9.13KK171 pKa = 9.55PEE173 pKa = 4.23EE174 pKa = 4.04EE175 pKa = 4.35VMDD178 pKa = 4.23AVHH181 pKa = 6.47NVDD184 pKa = 3.24SSNIGSHH191 pKa = 5.56TLDD194 pKa = 3.46SDD196 pKa = 4.41HH197 pKa = 7.09IHH199 pKa = 5.77NQYY202 pKa = 10.64INNEE206 pKa = 4.0DD207 pKa = 3.46VNLNNNDD214 pKa = 3.42TEE216 pKa = 4.38NYY218 pKa = 9.75NRR220 pKa = 11.84LDD222 pKa = 5.23DD223 pKa = 4.59ISDD226 pKa = 3.69SNDD229 pKa = 2.99YY230 pKa = 11.68DD231 pKa = 6.01DD232 pKa = 6.54FDD234 pKa = 6.78DD235 pKa = 6.24DD236 pKa = 4.83NFII239 pKa = 5.15

Molecular weight: 26.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172WD02|A0A172WD02_BUCSC DNA replication protein DnaC OS=Buchnera aphidicola subsp. Schlechtendalia chinensis OX=118110 GN=XW81_00110 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 6.96GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.89NGRR28 pKa = 11.84HH29 pKa = 4.78ILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.84FRR39 pKa = 11.84MHH41 pKa = 6.45LTVSSFKK48 pKa = 11.03

Molecular weight: 5.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

499

0

499

160319

38

1403

321.3

36.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.053 ± 0.086

1.449 ± 0.041

4.086 ± 0.062

5.046 ± 0.088

5.226 ± 0.119

5.402 ± 0.097

2.085 ± 0.044

11.486 ± 0.129

9.888 ± 0.144

10.039 ± 0.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.203 ± 0.037

7.464 ± 0.098

2.911 ± 0.047

2.857 ± 0.058

3.764 ± 0.081

7.683 ± 0.093

4.6 ± 0.063

5.313 ± 0.095

0.88 ± 0.042

3.564 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski