Halomonas muralis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3825 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9RWQ2|A0A1G9RWQ2_9GAMM N-succinylarginine dihydrolase OS=Halomonas muralis OX=119000 GN=astB PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 9.06KK3 pKa = 8.27TLLATAIAGALGASAAAQAATVYY26 pKa = 10.54NQDD29 pKa = 2.95GTQLDD34 pKa = 3.83IYY36 pKa = 11.21GNIQIAYY43 pKa = 8.61YY44 pKa = 10.62SLDD47 pKa = 3.71AGDD50 pKa = 4.19EE51 pKa = 4.42SVDD54 pKa = 3.92EE55 pKa = 4.38IADD58 pKa = 3.64NGSTFGFAAQHH69 pKa = 5.85VINDD73 pKa = 3.85GLTGYY78 pKa = 10.81LKK80 pKa = 11.2LEE82 pKa = 4.31FDD84 pKa = 4.44DD85 pKa = 6.14FKK87 pKa = 11.61ADD89 pKa = 4.07EE90 pKa = 4.27IKK92 pKa = 11.09VRR94 pKa = 11.84GRR96 pKa = 11.84DD97 pKa = 3.36AGDD100 pKa = 3.13QAYY103 pKa = 10.69VGLKK107 pKa = 10.76GNFGDD112 pKa = 5.01ARR114 pKa = 11.84LGSYY118 pKa = 10.69DD119 pKa = 4.28PLIDD123 pKa = 5.54DD124 pKa = 5.75WIQDD128 pKa = 4.02PITNNEE134 pKa = 3.86FADD137 pKa = 3.85VSDD140 pKa = 3.95SSGLIVDD147 pKa = 4.63IYY149 pKa = 11.13GADD152 pKa = 3.57DD153 pKa = 3.83RR154 pKa = 11.84EE155 pKa = 4.24GDD157 pKa = 3.43KK158 pKa = 10.87FQYY161 pKa = 9.55TSPSFGGLQFAVGTQYY177 pKa = 11.35KK178 pKa = 10.6GDD180 pKa = 3.96AEE182 pKa = 4.23EE183 pKa = 4.34KK184 pKa = 10.34NAFIEE189 pKa = 4.3ADD191 pKa = 3.19GRR193 pKa = 11.84ASFFSGVKK201 pKa = 7.36YY202 pKa = 9.14TVGAFSIAGVYY213 pKa = 10.57DD214 pKa = 3.36NLAIYY219 pKa = 10.54DD220 pKa = 4.12GEE222 pKa = 4.56FNTAVDD228 pKa = 3.51VDD230 pKa = 4.0GDD232 pKa = 3.96GTADD236 pKa = 3.1IAAGDD241 pKa = 3.99DD242 pKa = 4.3FEE244 pKa = 7.6AGDD247 pKa = 3.79QYY249 pKa = 11.83GVTAQYY255 pKa = 11.28TMGALRR261 pKa = 11.84VALKK265 pKa = 9.21YY266 pKa = 10.29EE267 pKa = 4.3RR268 pKa = 11.84YY269 pKa = 8.47EE270 pKa = 4.33SEE272 pKa = 4.45VEE274 pKa = 4.5DD275 pKa = 3.73VQPDD279 pKa = 3.52VNFYY283 pKa = 11.25GIGARR288 pKa = 11.84YY289 pKa = 8.88GYY291 pKa = 11.51GMGDD295 pKa = 2.98IYY297 pKa = 11.28AAYY300 pKa = 9.82QYY302 pKa = 11.46VDD304 pKa = 2.88IGGSDD309 pKa = 3.65LEE311 pKa = 4.34EE312 pKa = 4.44TIEE315 pKa = 4.85EE316 pKa = 4.08DD317 pKa = 5.0DD318 pKa = 4.22EE319 pKa = 5.55NIGEE323 pKa = 4.3NDD325 pKa = 3.48DD326 pKa = 3.76SRR328 pKa = 11.84NEE330 pKa = 3.77ILVGVTYY337 pKa = 10.63NVSPAMYY344 pKa = 8.43VWLEE348 pKa = 3.74GGVRR352 pKa = 11.84DD353 pKa = 5.2QEE355 pKa = 4.9DD356 pKa = 3.7DD357 pKa = 4.33LGDD360 pKa = 4.05FTATGVTYY368 pKa = 10.81SFF370 pKa = 4.48

Molecular weight: 39.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9L1K9|A0A1G9L1K9_9GAMM Nucleobase:cation symporter-1 NCS1 family OS=Halomonas muralis OX=119000 GN=SAMN05661010_01983 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38GGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3825

0

3825

1244899

39

4634

325.5

35.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.315 ± 0.051

0.97 ± 0.014

5.822 ± 0.038

6.247 ± 0.036

3.52 ± 0.028

8.273 ± 0.04

2.417 ± 0.02

4.972 ± 0.029

2.691 ± 0.031

11.36 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.479 ± 0.018

2.631 ± 0.025

4.89 ± 0.026

3.772 ± 0.029

7.179 ± 0.051

5.474 ± 0.025

4.988 ± 0.035

7.115 ± 0.03

1.468 ± 0.016

2.417 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski