Lake Sinai virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes; Nodamuvirales; Sinhaliviridae; Sinaivirus; unclassified Sinaivirus

Average proteome isoelectric point is 7.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGU1|A0A1L3KGU1_9VIRU Chropara_Vmeth domain-containing protein OS=Lake Sinai virus OX=1547219 PE=4 SV=1
MM1 pKa = 7.12VKK3 pKa = 10.35LVFAAFIALYY13 pKa = 9.16LVCQVPPISHH23 pKa = 7.43WIEE26 pKa = 3.68DD27 pKa = 4.15WIDD30 pKa = 4.63DD31 pKa = 4.07FDD33 pKa = 4.31TMCSYY38 pKa = 10.99EE39 pKa = 4.2YY40 pKa = 10.97AAADD44 pKa = 3.72AYY46 pKa = 11.49NSFVHH51 pKa = 5.45KK52 pKa = 10.37HH53 pKa = 4.85RR54 pKa = 11.84VAAYY58 pKa = 7.84AAGVRR63 pKa = 11.84VYY65 pKa = 10.38RR66 pKa = 11.84YY67 pKa = 7.31RR68 pKa = 11.84TPWYY72 pKa = 8.93CVASRR77 pKa = 11.84TVPVVHH83 pKa = 6.67PFGWLMAKK91 pKa = 10.29YY92 pKa = 10.03EE93 pKa = 4.29STTYY97 pKa = 10.28HH98 pKa = 6.0VSEE101 pKa = 4.42LLDD104 pKa = 3.4RR105 pKa = 11.84LGIANATLSDD115 pKa = 3.58GLRR118 pKa = 11.84AVAEE122 pKa = 4.38TAFTAYY128 pKa = 9.11MYY130 pKa = 10.17YY131 pKa = 10.15QIVWFQLVFIILLTGSFIAIVCSSRR156 pKa = 11.84VRR158 pKa = 11.84VVRR161 pKa = 11.84VRR163 pKa = 11.84NRR165 pKa = 11.84VRR167 pKa = 11.84GYY169 pKa = 10.6SVAEE173 pKa = 3.74LRR175 pKa = 11.84AEE177 pKa = 4.06FEE179 pKa = 4.43DD180 pKa = 5.54AISDD184 pKa = 3.87VNAPPSRR191 pKa = 11.84GHH193 pKa = 6.65SGLTFQRR200 pKa = 11.84KK201 pKa = 7.41VVEE204 pKa = 4.48SWCLDD209 pKa = 3.05QLLRR213 pKa = 11.84YY214 pKa = 8.25FRR216 pKa = 11.84HH217 pKa = 5.0VRR219 pKa = 11.84FVSATCDD226 pKa = 2.59RR227 pKa = 11.84WAEE230 pKa = 3.93LGHH233 pKa = 6.76RR234 pKa = 11.84IHH236 pKa = 7.02RR237 pKa = 11.84CAPVVMDD244 pKa = 4.08GSFVPEE250 pKa = 4.03THH252 pKa = 6.69HH253 pKa = 6.98GGVGCRR259 pKa = 11.84RR260 pKa = 11.84APSNCPDD267 pKa = 3.78RR268 pKa = 11.84YY269 pKa = 10.66DD270 pKa = 3.56IPACVLSHH278 pKa = 6.23VDD280 pKa = 3.79YY281 pKa = 11.58YY282 pKa = 10.93MSPDD286 pKa = 3.42QLASTVVGPTFIVNHH301 pKa = 7.23DD302 pKa = 3.91YY303 pKa = 11.57SCVDD307 pKa = 3.4SLSVAEE313 pKa = 4.2ANIRR317 pKa = 11.84AAGGLITSSVRR328 pKa = 11.84DD329 pKa = 3.74GPVYY333 pKa = 10.58GPHH336 pKa = 7.68PYY338 pKa = 10.22HH339 pKa = 6.59LWDD342 pKa = 3.65NEE344 pKa = 4.13GVVVSSTGAFRR355 pKa = 11.84YY356 pKa = 9.71YY357 pKa = 10.35RR358 pKa = 11.84VGRR361 pKa = 11.84LYY363 pKa = 10.46DD364 pKa = 3.3TTLYY368 pKa = 10.9YY369 pKa = 10.9AFPVAGTYY377 pKa = 9.23TRR379 pKa = 11.84DD380 pKa = 3.27DD381 pKa = 3.88PTNLRR386 pKa = 11.84CSTVGDD392 pKa = 3.2FHH394 pKa = 8.25YY395 pKa = 10.72YY396 pKa = 9.93SPHH399 pKa = 5.6EE400 pKa = 4.2KK401 pKa = 10.45RR402 pKa = 11.84FISYY406 pKa = 10.41SVDD409 pKa = 2.91GNSYY413 pKa = 9.94HH414 pKa = 6.11VFGVSVPRR422 pKa = 11.84SLADD426 pKa = 3.09YY427 pKa = 10.3CAATFCRR434 pKa = 11.84SARR437 pKa = 11.84DD438 pKa = 3.55DD439 pKa = 3.42KK440 pKa = 11.38FYY442 pKa = 11.33DD443 pKa = 3.57SLRR446 pKa = 11.84SYY448 pKa = 9.17YY449 pKa = 9.02QNRR452 pKa = 11.84CRR454 pKa = 11.84AIGFTDD460 pKa = 3.92ARR462 pKa = 11.84DD463 pKa = 3.62TLILDD468 pKa = 4.81FIIHH472 pKa = 6.66LCDD475 pKa = 3.16EE476 pKa = 4.87ASLKK480 pKa = 10.08TFGFSRR486 pKa = 11.84LSTAPSSWTAYY497 pKa = 8.96CLSWVLVKK505 pKa = 10.45FNHH508 pKa = 6.01IMPLALTSFTVNALHH523 pKa = 7.11RR524 pKa = 11.84FFGAKK529 pKa = 8.97AAPWNWASIHH539 pKa = 6.35LPTYY543 pKa = 11.51DD544 pKa = 3.08MVTSPFRR551 pKa = 11.84LKK553 pKa = 10.38MFGRR557 pKa = 11.84NPTTFNLEE565 pKa = 3.83RR566 pKa = 11.84FRR568 pKa = 11.84AEE570 pKa = 3.81ATVVGAPNLGQSAAGASQDD589 pKa = 3.75SDD591 pKa = 3.19KK592 pKa = 11.55HH593 pKa = 6.64DD594 pKa = 3.47IQLGNPGAAAGSVSSSTFSDD614 pKa = 3.49SASASDD620 pKa = 5.72DD621 pKa = 3.33VLLDD625 pKa = 3.88HH626 pKa = 7.15EE627 pKa = 5.36SGSILDD633 pKa = 5.05DD634 pKa = 4.07SFCSSSPTYY643 pKa = 10.41GSSGVLSNKK652 pKa = 9.35GKK654 pKa = 10.45ARR656 pKa = 11.84RR657 pKa = 11.84KK658 pKa = 7.82PHH660 pKa = 6.73RR661 pKa = 11.84PTNRR665 pKa = 11.84DD666 pKa = 2.98PDD668 pKa = 3.62RR669 pKa = 11.84DD670 pKa = 3.64QEE672 pKa = 4.79HH673 pKa = 6.94NICHH677 pKa = 6.99PKK679 pKa = 8.42RR680 pKa = 11.84TTYY683 pKa = 11.18ADD685 pKa = 3.91CPDD688 pKa = 3.66PTASCGPHH696 pKa = 6.48FFMSVCEE703 pKa = 4.06RR704 pKa = 11.84DD705 pKa = 3.33EE706 pKa = 4.23SVPTLFHH713 pKa = 5.9AHH715 pKa = 6.13AVGGEE720 pKa = 4.36DD721 pKa = 2.69ITHH724 pKa = 5.31VVEE727 pKa = 4.84PDD729 pKa = 2.96LGIAISEE736 pKa = 4.25RR737 pKa = 11.84FSASQLRR744 pKa = 11.84LLSWSIDD751 pKa = 3.36GVFNTLSRR759 pKa = 11.84AATSSFVEE767 pKa = 4.23SSLLSLLRR775 pKa = 11.84FMQQVPTTQPSAEE788 pKa = 3.96ARR790 pKa = 11.84RR791 pKa = 11.84CLLFGGPRR799 pKa = 11.84QRR801 pKa = 11.84FKK803 pKa = 11.27GYY805 pKa = 8.63DD806 pKa = 3.27TFDD809 pKa = 3.68LGFMGISVPTSKK821 pKa = 9.56TAGVASCLRR830 pKa = 11.84EE831 pKa = 3.94VARR834 pKa = 11.84QHH836 pKa = 6.28ACADD840 pKa = 3.94SPHH843 pKa = 6.91EE844 pKa = 4.38GPKK847 pKa = 10.29LHH849 pKa = 6.95

Molecular weight: 94.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGN5|A0A1L3KGN5_9VIRU RNA-directed RNA polymerase OS=Lake Sinai virus OX=1547219 PE=4 SV=1
MM1 pKa = 7.17NQQQSTASPRR11 pKa = 11.84LARR14 pKa = 11.84PSPQAPTSRR23 pKa = 11.84SARR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84NRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84NGATAAVGPVAIQPSRR50 pKa = 11.84ITRR53 pKa = 11.84RR54 pKa = 11.84TVTNLTRR61 pKa = 11.84RR62 pKa = 11.84PFAITSAGLAWLRR75 pKa = 11.84QYY77 pKa = 10.73LNPMGPSMSSVAGFPDD93 pKa = 4.59GSAVTTCIADD103 pKa = 3.28YY104 pKa = 11.2SNNFNISFPPRR115 pKa = 11.84EE116 pKa = 4.35AIYY119 pKa = 8.7CTGTNSEE126 pKa = 4.18NQATLLDD133 pKa = 3.72AATYY137 pKa = 10.82AKK139 pKa = 9.89IDD141 pKa = 3.2AWTKK145 pKa = 11.36ADD147 pKa = 3.2ITLCILALPMLRR159 pKa = 11.84NVAMVRR165 pKa = 11.84LYY167 pKa = 8.26PTTPTAFTLSDD178 pKa = 4.74GIPNFVQRR186 pKa = 11.84FPNWSAFTTEE196 pKa = 5.19GKK198 pKa = 10.24LLNNGDD204 pKa = 4.01SPGYY208 pKa = 8.33IQSFIYY214 pKa = 10.08LPNVDD219 pKa = 3.94KK220 pKa = 11.02HH221 pKa = 6.81LSAARR226 pKa = 11.84GYY228 pKa = 10.13RR229 pKa = 11.84LLSRR233 pKa = 11.84GLTGIFTAPSLEE245 pKa = 4.15TQGFVTACQYY255 pKa = 10.41LAQGTVLTQTVGNDD269 pKa = 3.17FVQSVEE275 pKa = 4.04VNADD279 pKa = 3.33KK280 pKa = 10.45TVKK283 pKa = 10.27NVAGKK288 pKa = 9.55RR289 pKa = 11.84LHH291 pKa = 6.32YY292 pKa = 10.3SGPPKK297 pKa = 10.55YY298 pKa = 10.45VFPLEE303 pKa = 4.48GDD305 pKa = 3.17NCAPSSLVEE314 pKa = 4.57TYY316 pKa = 10.02HH317 pKa = 6.01QAYY320 pKa = 8.56QARR323 pKa = 11.84AVDD326 pKa = 4.3GFYY329 pKa = 10.36MPLLSSSRR337 pKa = 11.84DD338 pKa = 3.45NPFQSPRR345 pKa = 11.84PQPIAVYY352 pKa = 10.15NRR354 pKa = 11.84WYY356 pKa = 10.9YY357 pKa = 10.44RR358 pKa = 11.84GCLDD362 pKa = 5.74PIPASKK368 pKa = 10.77YY369 pKa = 10.32ADD371 pKa = 3.73GPSQYY376 pKa = 10.58FYY378 pKa = 11.45DD379 pKa = 4.93LNLADD384 pKa = 5.86DD385 pKa = 4.13VAPLYY390 pKa = 9.08NTGVVWMEE398 pKa = 4.18GISPNFSLKK407 pKa = 10.69LKK409 pKa = 8.98TRR411 pKa = 11.84TVIQYY416 pKa = 9.27IPTSGSVLANFTRR429 pKa = 11.84HH430 pKa = 5.55EE431 pKa = 4.0PTYY434 pKa = 11.03DD435 pKa = 4.04QIALDD440 pKa = 3.51AADD443 pKa = 4.57RR444 pKa = 11.84VRR446 pKa = 11.84SMMPHH451 pKa = 7.71AYY453 pKa = 8.62PAAYY457 pKa = 9.97NDD459 pKa = 3.89WGWLGDD465 pKa = 4.28LLDD468 pKa = 4.4STLSTLPGISMAYY481 pKa = 9.97RR482 pKa = 11.84FAKK485 pKa = 10.2PLIKK489 pKa = 9.98PAWNWLGGKK498 pKa = 9.81VSDD501 pKa = 4.29FFGNPVSRR509 pKa = 11.84DD510 pKa = 2.54GDD512 pKa = 3.36IFYY515 pKa = 10.47DD516 pKa = 3.98AKK518 pKa = 11.17

Molecular weight: 57.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2139

150

849

534.8

59.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.883 ± 0.644

2.665 ± 0.454

5.563 ± 0.493

2.899 ± 0.386

4.348 ± 0.614

5.517 ± 0.469

2.805 ± 0.623

4.254 ± 0.391

2.478 ± 0.382

9.724 ± 1.558

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.823 ± 0.198

3.226 ± 0.716

6.498 ± 0.649

2.712 ± 0.462

7.387 ± 0.248

9.21 ± 0.476

6.498 ± 0.339

7.246 ± 0.639

1.449 ± 0.131

4.815 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski