Neisseriaceae bacterium DSM 100970

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Aquella

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I7N935|A0A2I7N935_9NEIS Uncharacterized protein OS=Neisseriaceae bacterium DSM 100970 OX=2052837 GN=CUN60_11815 PE=4 SV=1
MM1 pKa = 6.83QKK3 pKa = 10.61KK4 pKa = 10.19IILLSMFVLNGCAWFSGPDD23 pKa = 3.54DD24 pKa = 4.99RR25 pKa = 11.84PPQQYY30 pKa = 8.39TPSYY34 pKa = 10.2QYY36 pKa = 11.07GNTPMGVYY44 pKa = 9.8VGNPEE49 pKa = 5.54DD50 pKa = 4.6IGDD53 pKa = 4.26DD54 pKa = 3.56MMGDD58 pKa = 4.16DD59 pKa = 5.23MYY61 pKa = 11.04MPDD64 pKa = 4.14GQPMGEE70 pKa = 4.07YY71 pKa = 9.52QAQSGQQEE79 pKa = 3.99QQFQYY84 pKa = 10.72IQGQEE89 pKa = 3.93EE90 pKa = 4.45LMADD94 pKa = 3.7QSKK97 pKa = 9.34QQIKK101 pKa = 10.16QYY103 pKa = 10.61NAEE106 pKa = 4.06QQIGEE111 pKa = 4.47SLTNQYY117 pKa = 7.85MQQIKK122 pKa = 9.7SQQGNNN128 pKa = 3.23

Molecular weight: 14.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I7N5V2|A0A2I7N5V2_9NEIS Periplasmic serine endoprotease DegP-like OS=Neisseriaceae bacterium DSM 100970 OX=2052837 GN=CUN60_05795 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTKK11 pKa = 10.59RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.88GFLVRR21 pKa = 11.84MKK23 pKa = 9.29TKK25 pKa = 10.48AGRR28 pKa = 11.84AIINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.86GRR39 pKa = 11.84KK40 pKa = 8.75SLAVV44 pKa = 3.3

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2454

0

2454

828413

29

4371

337.6

37.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.531 ± 0.049

1.14 ± 0.019

4.887 ± 0.04

5.093 ± 0.053

4.456 ± 0.045

6.589 ± 0.056

1.858 ± 0.027

8.391 ± 0.054

6.187 ± 0.05

10.062 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.02

6.324 ± 0.058

3.671 ± 0.031

3.84 ± 0.028

3.382 ± 0.039

7.148 ± 0.062

5.606 ± 0.059

6.385 ± 0.042

1.03 ± 0.017

4.094 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski