Amino acid dipepetide frequency for Neisseriaceae bacterium DSM 100970

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.923AlaAla: 5.923 ± 0.117	0.992AlaCys: 0.992 ± 0.042	3.598AlaAsp: 3.598 ± 0.063	3.933AlaGlu: 3.933 ± 0.081	2.86AlaPhe: 2.86 ± 0.061	5.512AlaGly: 5.512 ± 0.098	1.165AlaHis: 1.165 ± 0.034	6.663AlaIle: 6.663 ± 0.104	5.402AlaLys: 5.402 ± 0.099	6.777AlaLeu: 6.777 ± 0.095	1.799AlaMet: 1.799 ± 0.048	5.034AlaAsn: 5.034 ± 0.087	2.289AlaPro: 2.289 ± 0.059	2.493AlaGln: 2.493 ± 0.061	2.621AlaArg: 2.621 ± 0.064	5.06AlaSer: 5.06 ± 0.091	4.892AlaThr: 4.892 ± 0.099	4.74AlaVal: 4.74 ± 0.083	0.732AlaTrp: 0.732 ± 0.032	2.823AlaTyr: 2.823 ± 0.066	0.0AlaXaa: 0.0 ± 0.0
Cys	0.815CysAla: 0.815 ± 0.029	0.138CysCys: 0.138 ± 0.013	0.555CysAsp: 0.555 ± 0.027	0.49CysGlu: 0.49 ± 0.028	0.456CysPhe: 0.456 ± 0.024	1.085CysGly: 1.085 ± 0.035	0.237CysHis: 0.237 ± 0.018	0.82CysIle: 0.82 ± 0.036	0.606CysLys: 0.606 ± 0.029	1.047CysLeu: 1.047 ± 0.032	0.233CysMet: 0.233 ± 0.018	0.831CysAsn: 0.831 ± 0.036	0.495CysPro: 0.495 ± 0.037	0.486CysGln: 0.486 ± 0.025	0.361CysArg: 0.361 ± 0.023	0.945CysSer: 0.945 ± 0.04	0.593CysThr: 0.593 ± 0.031	0.602CysVal: 0.602 ± 0.024	0.116CysTrp: 0.116 ± 0.014	0.493CysTyr: 0.493 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	3.148AspAla: 3.148 ± 0.067	0.594AspCys: 0.594 ± 0.031	2.273AspAsp: 2.273 ± 0.061	2.801AspGlu: 2.801 ± 0.076	2.444AspPhe: 2.444 ± 0.06	3.347AspGly: 3.347 ± 0.076	0.805AspHis: 0.805 ± 0.033	3.953AspIle: 3.953 ± 0.076	3.94AspLys: 3.94 ± 0.09	4.802AspLeu: 4.802 ± 0.077	1.077AspMet: 1.077 ± 0.043	3.193AspAsn: 3.193 ± 0.07	1.711AspPro: 1.711 ± 0.047	1.459AspGln: 1.459 ± 0.039	1.526AspArg: 1.526 ± 0.046	3.423AspSer: 3.423 ± 0.077	2.349AspThr: 2.349 ± 0.055	2.795AspVal: 2.795 ± 0.067	0.643AspTrp: 0.643 ± 0.03	2.587AspTyr: 2.587 ± 0.07	0.0AspXaa: 0.0 ± 0.0
Glu	3.662GluAla: 3.662 ± 0.089	0.53GluCys: 0.53 ± 0.028	2.185GluAsp: 2.185 ± 0.053	2.968GluGlu: 2.968 ± 0.087	2.409GluPhe: 2.409 ± 0.064	2.214GluGly: 2.214 ± 0.058	1.148GluHis: 1.148 ± 0.04	4.599GluIle: 4.599 ± 0.101	3.345GluLys: 3.345 ± 0.079	6.237GluLeu: 6.237 ± 0.118	1.305GluMet: 1.305 ± 0.041	2.927GluAsn: 2.927 ± 0.059	1.301GluPro: 1.301 ± 0.046	2.168GluGln: 2.168 ± 0.056	1.965GluArg: 1.965 ± 0.052	3.458GluSer: 3.458 ± 0.079	2.366GluThr: 2.366 ± 0.057	3.498GluVal: 3.498 ± 0.07	0.503GluTrp: 0.503 ± 0.026	2.138GluTyr: 2.138 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	3.758PheAla: 3.758 ± 0.076	0.575PheCys: 0.575 ± 0.024	2.469PheAsp: 2.469 ± 0.06	1.927PheGlu: 1.927 ± 0.049	2.039PhePhe: 2.039 ± 0.063	3.244PheGly: 3.244 ± 0.068	0.804PheHis: 0.804 ± 0.032	4.162PheIle: 4.162 ± 0.092	2.32PheLys: 2.32 ± 0.06	3.868PheLeu: 3.868 ± 0.089	1.102PheMet: 1.102 ± 0.035	3.013PheAsn: 3.013 ± 0.072	1.535PhePro: 1.535 ± 0.048	1.16PheGln: 1.16 ± 0.036	1.394PheArg: 1.394 ± 0.041	3.576PheSer: 3.576 ± 0.071	2.616PheThr: 2.616 ± 0.063	2.689PheVal: 2.689 ± 0.06	0.55PheTrp: 0.55 ± 0.029	1.765PheTyr: 1.765 ± 0.053	0.0PheXaa: 0.0 ± 0.0
Gly	4.594GlyAla: 4.594 ± 0.093	0.903GlyCys: 0.903 ± 0.033	2.914GlyAsp: 2.914 ± 0.061	3.008GlyGlu: 3.008 ± 0.07	3.289GlyPhe: 3.289 ± 0.077	4.821GlyGly: 4.821 ± 0.099	1.223GlyHis: 1.223 ± 0.041	5.669GlyIle: 5.669 ± 0.09	4.37GlyLys: 4.37 ± 0.077	6.304GlyLeu: 6.304 ± 0.097	1.679GlyMet: 1.679 ± 0.053	3.97GlyAsn: 3.97 ± 0.088	1.2GlyPro: 1.2 ± 0.04	2.236GlyGln: 2.236 ± 0.054	2.128GlyArg: 2.128 ± 0.055	4.81GlySer: 4.81 ± 0.116	3.83GlyThr: 3.83 ± 0.101	5.043GlyVal: 5.043 ± 0.092	0.799GlyTrp: 0.799 ± 0.034	3.102GlyTyr: 3.102 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	1.195HisAla: 1.195 ± 0.036	0.214HisCys: 0.214 ± 0.018	1.045HisAsp: 1.045 ± 0.036	1.028HisGlu: 1.028 ± 0.038	0.794HisPhe: 0.794 ± 0.037	1.287HisGly: 1.287 ± 0.039	0.503HisHis: 0.503 ± 0.029	1.373HisIle: 1.373 ± 0.043	1.254HisLys: 1.254 ± 0.043	1.863HisLeu: 1.863 ± 0.051	0.398HisMet: 0.398 ± 0.022	1.153HisAsn: 1.153 ± 0.031	0.957HisPro: 0.957 ± 0.035	0.829HisGln: 0.829 ± 0.034	0.635HisArg: 0.635 ± 0.03	1.166HisSer: 1.166 ± 0.04	0.837HisThr: 0.837 ± 0.033	0.968HisVal: 0.968 ± 0.036	0.21HisTrp: 0.21 ± 0.016	0.873HisTyr: 0.873 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	7.173IleAla: 7.173 ± 0.116	1.082IleCys: 1.082 ± 0.036	4.673IleAsp: 4.673 ± 0.085	4.314IleGlu: 4.314 ± 0.083	3.47IlePhe: 3.47 ± 0.087	5.433IleGly: 5.433 ± 0.093	1.405IleHis: 1.405 ± 0.049	7.293IleIle: 7.293 ± 0.12	5.147IleLys: 5.147 ± 0.086	7.842IleLeu: 7.842 ± 0.119	1.793IleMet: 1.793 ± 0.052	5.734IleAsn: 5.734 ± 0.093	3.339IlePro: 3.339 ± 0.061	2.432IleGln: 2.432 ± 0.058	2.844IleArg: 2.844 ± 0.067	6.423IleSer: 6.423 ± 0.106	4.832IleThr: 4.832 ± 0.07	4.884IleVal: 4.884 ± 0.093	0.706IleTrp: 0.706 ± 0.029	3.095IleTyr: 3.095 ± 0.065	0.0IleXaa: 0.0 ± 0.0
Lys	4.29LysAla: 4.29 ± 0.086	0.507LysCys: 0.507 ± 0.027	3.037LysAsp: 3.037 ± 0.076	3.499LysGlu: 3.499 ± 0.078	2.886LysPhe: 2.886 ± 0.067	2.714LysGly: 2.714 ± 0.066	1.259LysHis: 1.259 ± 0.035	5.529LysIle: 5.529 ± 0.093	3.825LysLys: 3.825 ± 0.08	7.147LysLeu: 7.147 ± 0.121	1.697LysMet: 1.697 ± 0.046	3.851LysAsn: 3.851 ± 0.081	2.394LysPro: 2.394 ± 0.06	2.697LysGln: 2.697 ± 0.057	2.176LysArg: 2.176 ± 0.058	3.795LysSer: 3.795 ± 0.065	3.234LysThr: 3.234 ± 0.059	4.154LysVal: 4.154 ± 0.086	0.572LysTrp: 0.572 ± 0.027	2.61LysTyr: 2.61 ± 0.054	0.0LysXaa: 0.0 ± 0.0
Leu	8.79LeuAla: 8.79 ± 0.121	1.072LeuCys: 1.072 ± 0.036	5.176LeuAsp: 5.176 ± 0.094	5.177LeuGlu: 5.177 ± 0.094	4.612LeuPhe: 4.612 ± 0.085	6.401LeuGly: 6.401 ± 0.096	1.813LeuHis: 1.813 ± 0.048	8.528LeuIle: 8.528 ± 0.133	5.92LeuLys: 5.92 ± 0.083	9.944LeuLeu: 9.944 ± 0.135	2.243LeuMet: 2.243 ± 0.057	5.984LeuAsn: 5.984 ± 0.102	4.336LeuPro: 4.336 ± 0.078	3.595LeuGln: 3.595 ± 0.073	3.388LeuArg: 3.388 ± 0.077	7.348LeuSer: 7.348 ± 0.095	5.844LeuThr: 5.844 ± 0.094	6.214LeuVal: 6.214 ± 0.098	0.894LeuTrp: 0.894 ± 0.039	3.333LeuTyr: 3.333 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	1.964MetAla: 1.964 ± 0.053	0.202MetCys: 0.202 ± 0.013	0.932MetAsp: 0.932 ± 0.032	0.997MetGlu: 0.997 ± 0.036	0.932MetPhe: 0.932 ± 0.036	1.385MetGly: 1.385 ± 0.048	0.489MetHis: 0.489 ± 0.024	1.864MetIle: 1.864 ± 0.048	1.543MetLys: 1.543 ± 0.039	2.696MetLeu: 2.696 ± 0.058	0.566MetMet: 0.566 ± 0.03	1.304MetAsn: 1.304 ± 0.041	1.072MetPro: 1.072 ± 0.039	1.126MetGln: 1.126 ± 0.039	0.907MetArg: 0.907 ± 0.035	1.493MetSer: 1.493 ± 0.042	1.281MetThr: 1.281 ± 0.044	1.614MetVal: 1.614 ± 0.043	0.203MetTrp: 0.203 ± 0.014	0.69MetTyr: 0.69 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.903AsnAla: 3.903 ± 0.074	0.798AsnCys: 0.798 ± 0.038	2.802AsnAsp: 2.802 ± 0.055	2.766AsnGlu: 2.766 ± 0.063	2.516AsnPhe: 2.516 ± 0.062	4.58AsnGly: 4.58 ± 0.104	1.272AsnHis: 1.272 ± 0.038	4.739AsnIle: 4.739 ± 0.091	3.883AsnLys: 3.883 ± 0.068	6.368AsnLeu: 6.368 ± 0.096	1.319AsnMet: 1.319 ± 0.039	4.635AsnAsn: 4.635 ± 0.105	3.078AsnPro: 3.078 ± 0.075	3.294AsnGln: 3.294 ± 0.076	1.96AsnArg: 1.96 ± 0.045	4.872AsnSer: 4.872 ± 0.109	3.322AsnThr: 3.322 ± 0.079	3.271AsnVal: 3.271 ± 0.073	0.76AsnTrp: 0.76 ± 0.032	3.097AsnTyr: 3.097 ± 0.074	0.0AsnXaa: 0.0 ± 0.0
Pro	2.874ProAla: 2.874 ± 0.075	0.282ProCys: 0.282 ± 0.017	2.196ProAsp: 2.196 ± 0.055	2.799ProGlu: 2.799 ± 0.06	1.621ProPhe: 1.621 ± 0.045	2.225ProGly: 2.225 ± 0.056	0.687ProHis: 0.687 ± 0.028	2.618ProIle: 2.618 ± 0.06	1.97ProLys: 1.97 ± 0.052	3.549ProLeu: 3.549 ± 0.067	0.713ProMet: 0.713 ± 0.031	2.119ProAsn: 2.119 ± 0.055	0.936ProPro: 0.936 ± 0.04	1.48ProGln: 1.48 ± 0.043	1.107ProArg: 1.107 ± 0.037	2.424ProSer: 2.424 ± 0.063	2.301ProThr: 2.301 ± 0.112	2.996ProVal: 2.996 ± 0.059	0.338ProTrp: 0.338 ± 0.021	1.471ProTyr: 1.471 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	2.688GlnAla: 2.688 ± 0.058	0.315GlnCys: 0.315 ± 0.023	1.613GlnAsp: 1.613 ± 0.041	1.977GlnGlu: 1.977 ± 0.055	1.64GlnPhe: 1.64 ± 0.043	1.925GlnGly: 1.925 ± 0.046	0.715GlnHis: 0.715 ± 0.029	3.638GlnIle: 3.638 ± 0.072	2.432GlnLys: 2.432 ± 0.054	4.512GlnLeu: 4.512 ± 0.091	1.078GlnMet: 1.078 ± 0.04	2.415GlnAsn: 2.415 ± 0.065	1.271GlnPro: 1.271 ± 0.041	2.207GlnGln: 2.207 ± 0.073	1.129GlnArg: 1.129 ± 0.044	2.336GlnSer: 2.336 ± 0.059	2.017GlnThr: 2.017 ± 0.055	2.664GlnVal: 2.664 ± 0.065	0.334GlnTrp: 0.334 ± 0.022	1.496GlnTyr: 1.496 ± 0.044	0.0GlnXaa: 0.0 ± 0.0
Arg	2.003ArgAla: 2.003 ± 0.052	0.305ArgCys: 0.305 ± 0.018	1.711ArgAsp: 1.711 ± 0.05	2.14ArgGlu: 2.14 ± 0.059	1.654ArgPhe: 1.654 ± 0.04	1.96ArgGly: 1.96 ± 0.059	0.716ArgHis: 0.716 ± 0.03	2.776ArgIle: 2.776 ± 0.073	2.371ArgLys: 2.371 ± 0.054	3.745ArgLeu: 3.745 ± 0.082	0.91ArgMet: 0.91 ± 0.031	2.033ArgAsn: 2.033 ± 0.049	1.05ArgPro: 1.05 ± 0.038	1.549ArgGln: 1.549 ± 0.049	1.397ArgArg: 1.397 ± 0.047	1.902ArgSer: 1.902 ± 0.046	1.436ArgThr: 1.436 ± 0.041	2.269ArgVal: 2.269 ± 0.064	0.338ArgTrp: 0.338 ± 0.023	1.551ArgTyr: 1.551 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	5.037SerAla: 5.037 ± 0.098	1.016SerCys: 1.016 ± 0.046	3.212SerAsp: 3.212 ± 0.064	3.367SerGlu: 3.367 ± 0.081	3.363SerPhe: 3.363 ± 0.07	6.139SerGly: 6.139 ± 0.123	1.296SerHis: 1.296 ± 0.038	5.612SerIle: 5.612 ± 0.088	3.92SerLys: 3.92 ± 0.063	7.088SerLeu: 7.088 ± 0.094	1.555SerMet: 1.555 ± 0.044	4.39SerAsn: 4.39 ± 0.1	2.484SerPro: 2.484 ± 0.077	2.846SerGln: 2.846 ± 0.066	2.372SerArg: 2.372 ± 0.055	5.807SerSer: 5.807 ± 0.132	3.711SerThr: 3.711 ± 0.097	4.183SerVal: 4.183 ± 0.093	0.875SerTrp: 0.875 ± 0.039	3.21SerTyr: 3.21 ± 0.079	0.0SerXaa: 0.0 ± 0.0
Thr	4.331ThrAla: 4.331 ± 0.098	0.544ThrCys: 0.544 ± 0.03	2.817ThrAsp: 2.817 ± 0.068	2.527ThrGlu: 2.527 ± 0.064	2.102ThrPhe: 2.102 ± 0.059	4.252ThrGly: 4.252 ± 0.085	0.984ThrHis: 0.984 ± 0.035	4.82ThrIle: 4.82 ± 0.072	3.05ThrLys: 3.05 ± 0.054	5.374ThrLeu: 5.374 ± 0.078	1.178ThrMet: 1.178 ± 0.036	3.496ThrAsn: 3.496 ± 0.089	3.064ThrPro: 3.064 ± 0.121	1.947ThrGln: 1.947 ± 0.056	1.761ThrArg: 1.761 ± 0.048	3.923ThrSer: 3.923 ± 0.103	3.59ThrThr: 3.59 ± 0.103	3.782ThrVal: 3.782 ± 0.071	0.507ThrTrp: 0.507 ± 0.028	2.006ThrTyr: 2.006 ± 0.055	0.0ThrXaa: 0.0 ± 0.0
Val	5.444ValAla: 5.444 ± 0.081	0.694ValCys: 0.694 ± 0.03	3.211ValAsp: 3.211 ± 0.064	3.203ValGlu: 3.203 ± 0.073	2.904ValPhe: 2.904 ± 0.065	4.169ValGly: 4.169 ± 0.087	0.929ValHis: 0.929 ± 0.034	5.671ValIle: 5.671 ± 0.09	3.881ValLys: 3.881 ± 0.078	5.658ValLeu: 5.658 ± 0.088	1.596ValMet: 1.596 ± 0.052	4.068ValAsn: 4.068 ± 0.076	2.238ValPro: 2.238 ± 0.053	1.558ValGln: 1.558 ± 0.048	2.103ValArg: 2.103 ± 0.053	4.896ValSer: 4.896 ± 0.09	4.086ValThr: 4.086 ± 0.091	4.602ValVal: 4.602 ± 0.09	0.575ValTrp: 0.575 ± 0.029	2.366ValTyr: 2.366 ± 0.06	0.0ValXaa: 0.0 ± 0.0
Trp	0.633TrpAla: 0.633 ± 0.033	0.133TrpCys: 0.133 ± 0.014	0.465TrpAsp: 0.465 ± 0.027	0.424TrpGlu: 0.424 ± 0.024	0.602TrpPhe: 0.602 ± 0.031	0.639TrpGly: 0.639 ± 0.035	0.285TrpHis: 0.285 ± 0.018	0.74TrpIle: 0.74 ± 0.032	0.438TrpLys: 0.438 ± 0.025	1.339TrpLeu: 1.339 ± 0.047	0.227TrpMet: 0.227 ± 0.019	0.561TrpAsn: 0.561 ± 0.031	0.243TrpPro: 0.243 ± 0.017	0.705TrpGln: 0.705 ± 0.029	0.456TrpArg: 0.456 ± 0.024	0.642TrpSer: 0.642 ± 0.028	0.501TrpThr: 0.501 ± 0.03	0.655TrpVal: 0.655 ± 0.03	0.168TrpTrp: 0.168 ± 0.015	0.441TrpTyr: 0.441 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.082TyrAla: 3.082 ± 0.07	0.507TyrCys: 0.507 ± 0.026	1.987TyrAsp: 1.987 ± 0.06	1.584TyrGlu: 1.584 ± 0.049	1.969TyrPhe: 1.969 ± 0.049	2.562TyrGly: 2.562 ± 0.053	0.847TyrHis: 0.847 ± 0.034	2.846TyrIle: 2.846 ± 0.062	2.256TyrLys: 2.256 ± 0.055	4.458TyrLeu: 4.458 ± 0.084	0.788TyrMet: 0.788 ± 0.031	2.527TyrAsn: 2.527 ± 0.059	1.714TyrPro: 1.714 ± 0.052	2.135TyrGln: 2.135 ± 0.048	1.586TyrArg: 1.586 ± 0.046	3.183TyrSer: 3.183 ± 0.065	2.418TyrThr: 2.418 ± 0.065	2.227TyrVal: 2.227 ± 0.049	0.471TyrTrp: 0.471 ± 0.026	1.79TyrTyr: 1.79 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2454 proteins (828413 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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