Rhodobacteraceae bacterium SH-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pukyongiella

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3994 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S0MN55|A0A2S0MN55_9RHOB Amidohydrolase OS=Rhodobacteraceae bacterium SH-1 OX=2605946 GN=C6Y53_06065 PE=4 SV=1
MM1 pKa = 7.37SKK3 pKa = 10.31SRR5 pKa = 11.84NIVLGAVLAAGVTGPVLAGPLGLGRR30 pKa = 11.84PATPDD35 pKa = 3.74EE36 pKa = 4.04IAAWDD41 pKa = 3.71TDD43 pKa = 3.46VRR45 pKa = 11.84PDD47 pKa = 3.55GTGLPDD53 pKa = 3.78GEE55 pKa = 4.28GDD57 pKa = 3.19VWTGEE62 pKa = 4.08EE63 pKa = 4.55LFEE66 pKa = 4.76ANCAVCHH73 pKa = 6.39GDD75 pKa = 3.28FAEE78 pKa = 6.41GVDD81 pKa = 4.17NWPQLAGGFGTLDD94 pKa = 2.93QDD96 pKa = 5.02DD97 pKa = 4.04PVKK100 pKa = 10.14TVGSYY105 pKa = 8.23WPYY108 pKa = 11.5LSTTWDD114 pKa = 3.61YY115 pKa = 11.18IYY117 pKa = 11.11RR118 pKa = 11.84SMPYY122 pKa = 10.76GNAQSLEE129 pKa = 4.13PVDD132 pKa = 5.05VYY134 pKa = 11.91ALVAYY139 pKa = 9.63ILYY142 pKa = 11.02SNDD145 pKa = 3.33LVDD148 pKa = 5.71DD149 pKa = 4.66DD150 pKa = 5.66FVLSRR155 pKa = 11.84DD156 pKa = 3.36NFLDD160 pKa = 3.48VEE162 pKa = 4.4MPNAGGFRR170 pKa = 11.84VDD172 pKa = 4.94DD173 pKa = 4.03RR174 pKa = 11.84AEE176 pKa = 3.9TEE178 pKa = 4.06YY179 pKa = 10.48PLWRR183 pKa = 11.84GEE185 pKa = 3.99PCMEE189 pKa = 4.07NCKK192 pKa = 10.61DD193 pKa = 3.5AVTVTMRR200 pKa = 11.84AAVLDD205 pKa = 3.81VTPDD209 pKa = 3.53DD210 pKa = 4.05GAEE213 pKa = 3.79

Molecular weight: 23.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S0MRT3|A0A2S0MRT3_9RHOB Flagellar hook-length control protein FliK OS=Rhodobacteraceae bacterium SH-1 OX=2605946 GN=C6Y53_13220 PE=4 SV=2
MM1 pKa = 7.31LRR3 pKa = 11.84STMLAPAQTAAIGIKK18 pKa = 10.54AMLKK22 pKa = 9.88GRR24 pKa = 11.84RR25 pKa = 11.84SVIAGGMNRR34 pKa = 11.84LTAFSSRR41 pKa = 11.84LMPRR45 pKa = 11.84GVMLRR50 pKa = 11.84MMGRR54 pKa = 11.84SLRR57 pKa = 11.84NASS60 pKa = 3.23

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3994

0

3994

1272518

35

3759

318.6

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.839 ± 0.061

0.921 ± 0.012

6.454 ± 0.041

5.512 ± 0.041

3.598 ± 0.024

9.04 ± 0.038

2.069 ± 0.019

4.94 ± 0.031

2.602 ± 0.035

10.113 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.723 ± 0.017

2.451 ± 0.024

5.296 ± 0.031

3.001 ± 0.02

7.521 ± 0.045

4.818 ± 0.027

5.305 ± 0.025

7.259 ± 0.033

1.4 ± 0.017

2.138 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski