Rickettsiales endosymbiont of Stachyamoeba lipophora

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; unclassified Rickettsiales

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1519 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8T7U7|A0A3Q8T7U7_9RICK Glutamine--fructose-6-phosphate aminotransferase [isomerizing] OS=Rickettsiales endosymbiont of Stachyamoeba lipophora OX=2486578 GN=glmS PE=4 SV=1
MM1 pKa = 7.65AYY3 pKa = 10.24VVTDD7 pKa = 3.22NCIKK11 pKa = 10.54CKK13 pKa = 10.0YY14 pKa = 7.47TDD16 pKa = 3.85CVEE19 pKa = 4.15VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.18NMLVINPDD38 pKa = 2.97EE39 pKa = 5.49CIDD42 pKa = 4.08CGVCEE47 pKa = 4.37PEE49 pKa = 4.74CPVEE53 pKa = 4.68AISPEE58 pKa = 4.35SEE60 pKa = 5.43DD61 pKa = 3.76LLDD64 pKa = 3.44WLEE67 pKa = 4.02LNKK70 pKa = 10.37EE71 pKa = 4.38YY72 pKa = 10.93SEE74 pKa = 3.93KK75 pKa = 10.11WPNINRR81 pKa = 11.84KK82 pKa = 9.1KK83 pKa = 10.51DD84 pKa = 3.59QLPEE88 pKa = 3.72AVLFKK93 pKa = 10.87DD94 pKa = 3.92KK95 pKa = 10.7PNKK98 pKa = 9.67LEE100 pKa = 4.61EE101 pKa = 4.29YY102 pKa = 10.35FSPKK106 pKa = 9.18PGKK109 pKa = 10.1

Molecular weight: 12.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8T2W8|A0A3S8T2W8_9RICK Uncharacterized protein OS=Rickettsiales endosymbiont of Stachyamoeba lipophora OX=2486578 GN=EF513_03950 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.14QPSNIVRR12 pKa = 11.84KK13 pKa = 9.12RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.72GFRR19 pKa = 11.84SRR21 pKa = 11.84MATLGGRR28 pKa = 11.84KK29 pKa = 9.17VIAARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.37KK37 pKa = 10.45GRR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 8.7LTAA44 pKa = 3.95

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1519

0

1519

502053

27

4486

330.5

37.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.024 ± 0.069

0.939 ± 0.021

4.846 ± 0.04

6.228 ± 0.07

4.576 ± 0.043

5.441 ± 0.07

2.149 ± 0.029

9.53 ± 0.063

7.67 ± 0.065

10.487 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.065 ± 0.031

6.718 ± 0.071

3.353 ± 0.035

4.154 ± 0.055

3.368 ± 0.042

6.419 ± 0.046

5.281 ± 0.07

5.361 ± 0.059

0.765 ± 0.019

3.627 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski