BtVs-BetaCoV/SC2013

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Merbecovirus; Middle East respiratory syndrome-related coronavirus

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A023YAI9|A0A023YAI9_MERS ORF4a OS=BtVs-BetaCoV/SC2013 OX=1495253 PE=4 SV=1
MM1 pKa = 6.72TTIPTLSSLEE11 pKa = 4.06AEE13 pKa = 4.5EE14 pKa = 4.71EE15 pKa = 4.16LLSLDD20 pKa = 4.06QLPITPSPGTLALPNTGRR38 pKa = 11.84SLFPFHH44 pKa = 7.69LDD46 pKa = 2.79RR47 pKa = 11.84AYY49 pKa = 11.36LLMPIQPLHH58 pKa = 6.44KK59 pKa = 9.24MLGIGGDD66 pKa = 3.19RR67 pKa = 11.84TEE69 pKa = 4.68RR70 pKa = 11.84LIQEE74 pKa = 4.42MEE76 pKa = 3.95QSSWLPGGSSTTLEE90 pKa = 4.6PGLKK94 pKa = 8.77LTSLSEE100 pKa = 4.06LSRR103 pKa = 11.84MEE105 pKa = 3.63SSGYY109 pKa = 7.78MKK111 pKa = 10.4KK112 pKa = 10.21VLQTLPPVLGRR123 pKa = 11.84VTLTTIRR130 pKa = 11.84LLLHH134 pKa = 6.83SSLQVLGFLKK144 pKa = 10.19TSTLRR149 pKa = 11.84ALEE152 pKa = 4.06ATVSHH157 pKa = 6.09LQEE160 pKa = 4.25HH161 pKa = 6.97LAPAEE166 pKa = 3.99ALQDD170 pKa = 3.79LAQEE174 pKa = 4.2VLVPEE179 pKa = 4.26THH181 pKa = 7.16PEE183 pKa = 3.91VLPQVQLEE191 pKa = 4.43SEE193 pKa = 4.34LL194 pKa = 3.84

Molecular weight: 21.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A023YAJ4|A0A023YAJ4_MERS Nucleoprotein OS=BtVs-BetaCoV/SC2013 OX=1495253 GN=N PE=3 SV=1
MM1 pKa = 7.35ATPAAPRR8 pKa = 11.84AVTFGDD14 pKa = 3.57NNDD17 pKa = 3.83NNTNTQQSRR26 pKa = 11.84GRR28 pKa = 11.84GRR30 pKa = 11.84TPKK33 pKa = 9.93PRR35 pKa = 11.84PAPNNTVSWYY45 pKa = 9.65TGLTQHH51 pKa = 6.36GKK53 pKa = 9.97VPLSFPPGQGVPLNANSTPAQNAGYY78 pKa = 9.33WRR80 pKa = 11.84RR81 pKa = 11.84QDD83 pKa = 3.96RR84 pKa = 11.84KK85 pKa = 10.03INTGNGTKK93 pKa = 9.97QLAPRR98 pKa = 11.84WFFYY102 pKa = 9.92YY103 pKa = 10.27TGTGPEE109 pKa = 3.82ANLPFRR115 pKa = 11.84AVKK118 pKa = 10.55DD119 pKa = 4.02GIVWVHH125 pKa = 5.98EE126 pKa = 4.53EE127 pKa = 4.31GATDD131 pKa = 3.65APSSFGTRR139 pKa = 11.84NPNNDD144 pKa = 3.67PAIVTQFAPGTRR156 pKa = 11.84LPKK159 pKa = 10.18NFHH162 pKa = 6.51IEE164 pKa = 4.18GTGGNSQSSSRR175 pKa = 11.84ASSASRR181 pKa = 11.84GSSRR185 pKa = 11.84SSSRR189 pKa = 11.84GARR192 pKa = 11.84SGNSSRR198 pKa = 11.84SASPGPAGIGAVGGDD213 pKa = 3.62ASSILYY219 pKa = 10.5LSLLKK224 pKa = 10.62RR225 pKa = 11.84LEE227 pKa = 4.15DD228 pKa = 3.77LEE230 pKa = 4.75AGKK233 pKa = 10.6SKK235 pKa = 10.58SAPKK239 pKa = 10.58VVTKK243 pKa = 10.61KK244 pKa = 10.57DD245 pKa = 2.83AAAAKK250 pKa = 10.32NKK252 pKa = 8.56MRR254 pKa = 11.84HH255 pKa = 5.38KK256 pKa = 10.5RR257 pKa = 11.84VATKK261 pKa = 10.46SFNMVQAFGRR271 pKa = 11.84RR272 pKa = 11.84GPGDD276 pKa = 3.38LQGNFGDD283 pKa = 4.05LQLNKK288 pKa = 10.39LGTEE292 pKa = 4.19DD293 pKa = 4.25PRR295 pKa = 11.84WPQIAEE301 pKa = 4.2LAPSASAFMGMSQFKK316 pKa = 8.93LTHH319 pKa = 5.96QSNDD323 pKa = 3.01ADD325 pKa = 3.72GSPVYY330 pKa = 10.18FLRR333 pKa = 11.84YY334 pKa = 9.46SGAIKK339 pKa = 10.61LDD341 pKa = 3.7PKK343 pKa = 10.6NPNYY347 pKa = 9.39QKK349 pKa = 10.66WMEE352 pKa = 4.12LLEE355 pKa = 4.45ANIDD359 pKa = 3.68AYY361 pKa = 10.22KK362 pKa = 10.57SFPVKK367 pKa = 10.0EE368 pKa = 4.46KK369 pKa = 10.56KK370 pKa = 9.98QKK372 pKa = 11.13ADD374 pKa = 3.87DD375 pKa = 3.98NKK377 pKa = 10.54QDD379 pKa = 3.58STEE382 pKa = 4.13DD383 pKa = 3.52SGDD386 pKa = 3.53LFAEE390 pKa = 4.84AVASAMASQSLPQRR404 pKa = 11.84APKK407 pKa = 10.63GSITQRR413 pKa = 11.84SRR415 pKa = 11.84APRR418 pKa = 11.84APVSQMEE425 pKa = 4.86DD426 pKa = 3.1VNQTDD431 pKa = 3.8DD432 pKa = 3.56RR433 pKa = 11.84QQ434 pKa = 3.32

Molecular weight: 46.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

10106

82

7179

1010.6

112.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.896 ± 0.451

3.107 ± 0.554

5.333 ± 0.51

3.948 ± 0.298

4.938 ± 0.372

5.897 ± 0.42

2.058 ± 0.375

4.453 ± 0.441

5.274 ± 0.684

9.321 ± 0.659

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.132

5.096 ± 0.343

4.532 ± 0.801

3.82 ± 0.712

3.859 ± 0.543

7.263 ± 0.709

6.471 ± 0.193

8.698 ± 0.772

1.118 ± 0.217

4.73 ± 0.512
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski