Rotavirus A (strain RVA/Cow/United Kingdom/UK/1975/G6P7[5]) (RV-A)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A; Bovine rotavirus A; Bovine rotavirus G6

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03534-2|VP7-2_ROTBU Isoform of P03534 Isoform 2 of Outer capsid glycoprotein VP7 OS=Rotavirus A (strain RVA/Cow/United Kingdom/UK/1975/G6P7[5]) OX=10934 PE=2 SV=1
MM1 pKa = 8.16DD2 pKa = 4.71YY3 pKa = 10.55IIYY6 pKa = 10.24RR7 pKa = 11.84FLLIVVVLATMINAQNYY24 pKa = 7.95GVNLPITGSMDD35 pKa = 3.06TAYY38 pKa = 10.66ANSTQSEE45 pKa = 4.42PFLTSTLCLYY55 pKa = 10.92YY56 pKa = 9.93PVEE59 pKa = 4.38ASNEE63 pKa = 3.97IADD66 pKa = 4.42TEE68 pKa = 4.41WKK70 pKa = 8.51DD71 pKa = 3.48TLSQLFLTKK80 pKa = 10.05GWPTGSVYY88 pKa = 10.44FKK90 pKa = 10.34EE91 pKa = 4.39YY92 pKa = 10.03TDD94 pKa = 3.19IAAFSVEE101 pKa = 3.86PQLYY105 pKa = 9.89CDD107 pKa = 3.87YY108 pKa = 11.21NLVLMKK114 pKa = 10.43YY115 pKa = 10.56DD116 pKa = 3.62STQEE120 pKa = 3.79LDD122 pKa = 3.4MSEE125 pKa = 4.16LADD128 pKa = 5.13LILNEE133 pKa = 4.24WLCNPMDD140 pKa = 3.29ITLYY144 pKa = 10.67YY145 pKa = 9.25YY146 pKa = 10.55QQTDD150 pKa = 3.57EE151 pKa = 4.17ANKK154 pKa = 8.08WISMGSSCTVKK165 pKa = 10.28VCPLNTQTLGIGCLITNPDD184 pKa = 3.37TFEE187 pKa = 4.14TVATTEE193 pKa = 4.21KK194 pKa = 10.92LVITDD199 pKa = 3.3VVDD202 pKa = 4.33GVNHH206 pKa = 6.76KK207 pKa = 11.09LNVTTATCTIRR218 pKa = 11.84NCKK221 pKa = 9.83KK222 pKa = 10.18LGPRR226 pKa = 11.84EE227 pKa = 3.94NVAIIQVGGANVLDD241 pKa = 3.77ITADD245 pKa = 3.5PTTAPQTEE253 pKa = 3.66RR254 pKa = 11.84MMRR257 pKa = 11.84INWKK261 pKa = 9.54KK262 pKa = 7.26WWQVFYY268 pKa = 10.66TVVDD272 pKa = 3.52YY273 pKa = 11.46VNQIIQTMSKK283 pKa = 10.22RR284 pKa = 11.84SRR286 pKa = 11.84SLNSSAFYY294 pKa = 11.0YY295 pKa = 10.28RR296 pKa = 11.84VV297 pKa = 3.04

Molecular weight: 33.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P12474|VP4_ROTBU Outer capsid protein VP4 OS=Rotavirus A (strain RVA/Cow/United Kingdom/UK/1975/G6P7[5]) OX=10934 PE=3 SV=1
MM1 pKa = 7.27NHH3 pKa = 6.21LQQRR7 pKa = 11.84QLFLEE12 pKa = 4.16NLLVGVNNMFHH23 pKa = 6.33QMQKK27 pKa = 10.4RR28 pKa = 11.84PVNTCCRR35 pKa = 11.84SLQKK39 pKa = 10.64ILDD42 pKa = 3.97HH43 pKa = 7.17LILLQTIHH51 pKa = 6.63SPAFRR56 pKa = 11.84LDD58 pKa = 3.53QMQLRR63 pKa = 11.84QMQTLACLWIHH74 pKa = 6.26QYY76 pKa = 10.72NHH78 pKa = 6.34DD79 pKa = 3.87HH80 pKa = 5.81QVTLGAIKK88 pKa = 9.95WISPLIKK95 pKa = 9.9EE96 pKa = 4.73LKK98 pKa = 10.03

Molecular weight: 11.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

1

13

6192

98

1088

476.3

54.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.346 ± 0.438

1.26 ± 0.329

5.975 ± 0.341

5.636 ± 0.288

4.167 ± 0.284

3.569 ± 0.356

1.486 ± 0.31

7.348 ± 0.378

6.411 ± 0.572

9.222 ± 0.472

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.198 ± 0.209

6.912 ± 0.321

3.23 ± 0.226

4.328 ± 0.482

4.797 ± 0.281

7.607 ± 0.827

6.395 ± 0.551

6.767 ± 0.297

1.211 ± 0.148

5.136 ± 0.471
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski