Methylosinus sp. 3S-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylocystaceae; Methylosinus; unclassified Methylosinus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4187 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A6FT95|A0A1A6FT95_9RHIZ Glycosyl transferase family 2 OS=Methylosinus sp. 3S-1 OX=1849840 GN=A8B73_00475 PE=4 SV=1
MM1 pKa = 7.2TATALFNTSVMGMAAQASALGAVSQNIANSGTVGYY36 pKa = 10.62KK37 pKa = 10.43DD38 pKa = 3.17ATTQFSTVLSSVQNGSDD55 pKa = 3.37PAGGVTAQTGVAVTAQGNTQQTSSTTDD82 pKa = 3.46LAIQGSGFFVVSDD95 pKa = 3.5SSGNTLLTRR104 pKa = 11.84AGSFEE109 pKa = 4.39PDD111 pKa = 2.97SEE113 pKa = 4.13GRR115 pKa = 11.84LVNKK119 pKa = 10.14AGYY122 pKa = 10.51YY123 pKa = 10.31LMGYY127 pKa = 9.2SADD130 pKa = 4.21DD131 pKa = 4.03ANSPSDD137 pKa = 3.3IAGLQTVHH145 pKa = 5.8VAIGSLIATPSTEE158 pKa = 4.03GSLTANLQQGADD170 pKa = 3.79VVDD173 pKa = 5.08SSALPSTNDD182 pKa = 2.71ASATYY187 pKa = 9.24TSKK190 pKa = 10.07TSVTAYY196 pKa = 10.57DD197 pKa = 3.46NYY199 pKa = 10.68GNSVKK204 pKa = 10.61LDD206 pKa = 3.41VYY208 pKa = 10.59FSKK211 pKa = 9.71TADD214 pKa = 4.11NEE216 pKa = 4.06WEE218 pKa = 4.05MAVYY222 pKa = 10.24DD223 pKa = 4.01SSKK226 pKa = 11.05ADD228 pKa = 3.34SSSGGFPYY236 pKa = 10.45SEE238 pKa = 4.93GPLTTQSLSFDD249 pKa = 3.99SNGALTSGSPTTIDD263 pKa = 3.43VPNGQTLTLDD273 pKa = 3.99LSGMTQLGSSFSVSDD288 pKa = 3.29PTINGNAAQAVASVNVDD305 pKa = 2.5SDD307 pKa = 3.59GTLNYY312 pKa = 10.24VLTGGQTVPAYY323 pKa = 9.44KK324 pKa = 10.13IPLANVSSPSGLQSVSGNAFATNASSGTAYY354 pKa = 10.53VGLPGTGSFGDD365 pKa = 3.61IQSKK369 pKa = 9.8SLEE372 pKa = 4.2SSTVDD377 pKa = 4.76LATQLSNMIVAQRR390 pKa = 11.84SYY392 pKa = 9.83TANSQVFQVASEE404 pKa = 4.21VMQVLNNLKK413 pKa = 10.44

Molecular weight: 41.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A6FRM0|A0A1A6FRM0_9RHIZ TIGR01620 family protein OS=Methylosinus sp. 3S-1 OX=1849840 GN=A8B73_06110 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.09QPSKK9 pKa = 8.8IVRR12 pKa = 11.84KK13 pKa = 9.24RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84NVLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4187

0

4187

1315237

28

4976

314.1

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.146 ± 0.068

0.897 ± 0.016

5.665 ± 0.026

5.985 ± 0.042

3.795 ± 0.026

8.363 ± 0.046

1.935 ± 0.018

4.7 ± 0.029

2.967 ± 0.032

10.323 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.967 ± 0.019

2.251 ± 0.028

5.27 ± 0.034

2.72 ± 0.024

8.075 ± 0.054

5.587 ± 0.038

4.888 ± 0.045

7.107 ± 0.031

1.227 ± 0.016

2.13 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski