Edwardsiella phage PEi20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Kanagawavirus; Edwardsiella virus PEi20

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B6VL09|A0A0B6VL09_9CAUD AAA domain-containing protein OS=Edwardsiella phage PEi20 OX=1608310 GN=nrdC.10 PE=4 SV=1
MM1 pKa = 7.89KK2 pKa = 10.37DD3 pKa = 3.22QIINLGDD10 pKa = 3.59GVEE13 pKa = 5.62DD14 pKa = 3.9DD15 pKa = 4.99LEE17 pKa = 4.21WRR19 pKa = 11.84LYY21 pKa = 11.09DD22 pKa = 4.54YY23 pKa = 10.46MVALAKK29 pKa = 10.29EE30 pKa = 4.35QGIEE34 pKa = 3.99WAVEE38 pKa = 3.99NAWGEE43 pKa = 4.1NTVVIGGVAFEE54 pKa = 4.47VQWQFVGLEE63 pKa = 4.02NTDD66 pKa = 3.64YY67 pKa = 11.48EE68 pKa = 4.28PVQNKK73 pKa = 7.14EE74 pKa = 4.41TGCTEE79 pKa = 4.29YY80 pKa = 10.6IPVGDD85 pKa = 4.49WFWEE89 pKa = 4.0YY90 pKa = 11.61DD91 pKa = 3.61DD92 pKa = 6.11APDD95 pKa = 5.19FEE97 pKa = 5.07VSSYY101 pKa = 7.83WRR103 pKa = 11.84EE104 pKa = 3.8HH105 pKa = 5.26

Molecular weight: 12.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B6VKW2|A0A0B6VKW2_9CAUD dCMP hydroxymethylase OS=Edwardsiella phage PEi20 OX=1608310 GN=42 PE=3 SV=1
MM1 pKa = 7.0KK2 pKa = 10.03TSEE5 pKa = 4.65SYY7 pKa = 10.91GKK9 pKa = 9.86HH10 pKa = 5.39PRR12 pKa = 11.84RR13 pKa = 11.84DD14 pKa = 3.05AMVFKK19 pKa = 9.83FVISVSGKK27 pKa = 7.49ATAYY31 pKa = 8.52YY32 pKa = 10.25RR33 pKa = 11.84PVRR36 pKa = 11.84MTKK39 pKa = 8.44RR40 pKa = 11.84QKK42 pKa = 10.0RR43 pKa = 11.84AAIKK47 pKa = 9.94EE48 pKa = 3.53FRR50 pKa = 11.84AYY52 pKa = 9.56IKK54 pKa = 10.71SGLAAHH60 pKa = 5.89QWHH63 pKa = 7.27DD64 pKa = 4.29LSCKK68 pKa = 9.46KK69 pKa = 10.6CKK71 pKa = 10.11SS72 pKa = 3.29

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

298

3

301

54866

38

1257

182.3

20.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.283 ± 0.171

1.072 ± 0.062

6.468 ± 0.112

7.156 ± 0.174

4.411 ± 0.105

6.468 ± 0.172

1.896 ± 0.085

6.596 ± 0.104

7.13 ± 0.212

7.343 ± 0.126

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.104 ± 0.089

5.047 ± 0.122

3.645 ± 0.1

3.614 ± 0.109

4.578 ± 0.106

5.867 ± 0.119

5.876 ± 0.193

6.902 ± 0.112

1.509 ± 0.06

4.033 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski