Alloscardovia macacae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Alloscardovia

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1550 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2T1C8|A0A1Y2T1C8_9BIFI 30S ribosomal protein S1 OS=Alloscardovia macacae OX=1160091 GN=ALMA_0746 PE=4 SV=1
MM1 pKa = 7.13AQDD4 pKa = 3.56YY5 pKa = 10.0DD6 pKa = 3.94SPRR9 pKa = 11.84SKK11 pKa = 11.3DD12 pKa = 3.25EE13 pKa = 4.33DD14 pKa = 3.82EE15 pKa = 4.63EE16 pKa = 4.92SIEE19 pKa = 4.05ALGNSSRR26 pKa = 11.84QNQSVDD32 pKa = 3.84LDD34 pKa = 4.0DD35 pKa = 6.06DD36 pKa = 4.36EE37 pKa = 5.39NALAEE42 pKa = 4.77GYY44 pKa = 8.06EE45 pKa = 4.3LPGSDD50 pKa = 5.2LSNEE54 pKa = 4.18DD55 pKa = 3.2ASVTVIPMQGDD66 pKa = 3.34EE67 pKa = 4.7FVCVEE72 pKa = 4.22CFLVKK77 pKa = 10.3HH78 pKa = 6.46RR79 pKa = 11.84SQLAYY84 pKa = 7.65TTDD87 pKa = 3.4EE88 pKa = 4.51GPVCKK93 pKa = 10.29EE94 pKa = 3.84CAAA97 pKa = 4.21

Molecular weight: 10.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2SYG9|A0A1Y2SYG9_9BIFI 50S ribosomal protein L19 OS=Alloscardovia macacae OX=1160091 GN=rplS PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.64HH16 pKa = 5.7GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1550

0

1550

560029

29

3181

361.3

39.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.859 ± 0.079

0.755 ± 0.02

5.976 ± 0.05

6.19 ± 0.063

3.564 ± 0.044

7.527 ± 0.053

2.232 ± 0.033

5.283 ± 0.051

3.812 ± 0.05

8.991 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.456 ± 0.03

3.268 ± 0.038

4.23 ± 0.032

3.688 ± 0.038

5.629 ± 0.058

6.84 ± 0.057

6.455 ± 0.073

7.979 ± 0.058

1.313 ± 0.023

2.953 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski