Amino acid dipepetide frequency for Methanospirillum lacunae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.759AlaAla: 5.759 ± 0.102
0.98AlaCys: 0.98 ± 0.036
4.234AlaAsp: 4.234 ± 0.073
4.43AlaGlu: 4.43 ± 0.068
2.59AlaPhe: 2.59 ± 0.055
6.156AlaGly: 6.156 ± 0.088
1.315AlaHis: 1.315 ± 0.035
5.854AlaIle: 5.854 ± 0.081
3.073AlaLys: 3.073 ± 0.063
6.784AlaLeu: 6.784 ± 0.098
1.986AlaMet: 1.986 ± 0.051
2.175AlaAsn: 2.175 ± 0.048
2.244AlaPro: 2.244 ± 0.055
1.854AlaGln: 1.854 ± 0.044
3.246AlaArg: 3.246 ± 0.062
4.466AlaSer: 4.466 ± 0.072
3.717AlaThr: 3.717 ± 0.072
5.338AlaVal: 5.338 ± 0.083
0.77AlaTrp: 0.77 ± 0.028
2.071AlaTyr: 2.071 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.848CysAla: 0.848 ± 0.033
0.279CysCys: 0.279 ± 0.017
0.699CysAsp: 0.699 ± 0.024
0.628CysGlu: 0.628 ± 0.023
0.471CysPhe: 0.471 ± 0.018
1.384CysGly: 1.384 ± 0.04
0.3CysHis: 0.3 ± 0.017
1.045CysIle: 1.045 ± 0.033
0.532CysLys: 0.532 ± 0.021
1.092CysLeu: 1.092 ± 0.037
0.334CysMet: 0.334 ± 0.018
0.582CysAsn: 0.582 ± 0.023
0.835CysPro: 0.835 ± 0.03
0.398CysGln: 0.398 ± 0.02
0.708CysArg: 0.708 ± 0.027
1.018CysSer: 1.018 ± 0.04
0.836CysThr: 0.836 ± 0.03
0.787CysVal: 0.787 ± 0.032
0.153CysTrp: 0.153 ± 0.01
0.389CysTyr: 0.389 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.926AspAla: 3.926 ± 0.07
0.612AspCys: 0.612 ± 0.02
2.827AspAsp: 2.827 ± 0.059
3.917AspGlu: 3.917 ± 0.066
2.06AspPhe: 2.06 ± 0.05
4.08AspGly: 4.08 ± 0.088
1.269AspHis: 1.269 ± 0.037
5.124AspIle: 5.124 ± 0.081
2.473AspLys: 2.473 ± 0.053
6.307AspLeu: 6.307 ± 0.077
1.248AspMet: 1.248 ± 0.037
2.044AspAsn: 2.044 ± 0.042
3.148AspPro: 3.148 ± 0.048
2.261AspGln: 2.261 ± 0.047
2.711AspArg: 2.711 ± 0.06
3.472AspSer: 3.472 ± 0.069
3.028AspThr: 3.028 ± 0.066
3.547AspVal: 3.547 ± 0.068
0.57AspTrp: 0.57 ± 0.024
1.79AspTyr: 1.79 ± 0.043
0.0AspXaa: 0.0 ± 0.0
Glu
4.253GluAla: 4.253 ± 0.079
0.644GluCys: 0.644 ± 0.027
3.067GluAsp: 3.067 ± 0.052
4.862GluGlu: 4.862 ± 0.086
2.189GluPhe: 2.189 ± 0.046
3.852GluGly: 3.852 ± 0.067
1.276GluHis: 1.276 ± 0.042
6.115GluIle: 6.115 ± 0.089
4.647GluLys: 4.647 ± 0.076
5.051GluLeu: 5.051 ± 0.085
1.922GluMet: 1.922 ± 0.044
2.783GluAsn: 2.783 ± 0.06
2.125GluPro: 2.125 ± 0.05
2.4GluGln: 2.4 ± 0.056
3.14GluArg: 3.14 ± 0.062
3.824GluSer: 3.824 ± 0.07
3.307GluThr: 3.307 ± 0.058
4.072GluVal: 4.072 ± 0.073
0.606GluTrp: 0.606 ± 0.029
2.174GluTyr: 2.174 ± 0.051
0.0GluXaa: 0.0 ± 0.0
Phe
2.581PheAla: 2.581 ± 0.051
0.644PheCys: 0.644 ± 0.024
2.176PheAsp: 2.176 ± 0.046
2.115PheGlu: 2.115 ± 0.047
2.084PhePhe: 2.084 ± 0.06
3.059PheGly: 3.059 ± 0.064
0.808PheHis: 0.808 ± 0.027
3.243PheIle: 3.243 ± 0.065
1.534PheLys: 1.534 ± 0.036
3.894PheLeu: 3.894 ± 0.076
0.831PheMet: 0.831 ± 0.034
1.602PheAsn: 1.602 ± 0.053
1.671PhePro: 1.671 ± 0.042
1.163PheGln: 1.163 ± 0.033
1.882PheArg: 1.882 ± 0.048
3.622PheSer: 3.622 ± 0.06
2.47PheThr: 2.47 ± 0.054
2.405PheVal: 2.405 ± 0.052
0.496PheTrp: 0.496 ± 0.026
1.265PheTyr: 1.265 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
4.625GlyAla: 4.625 ± 0.086
1.192GlyCys: 1.192 ± 0.037
4.178GlyAsp: 4.178 ± 0.086
4.323GlyGlu: 4.323 ± 0.073
2.993GlyPhe: 2.993 ± 0.06
5.063GlyGly: 5.063 ± 0.094
1.274GlyHis: 1.274 ± 0.037
7.017GlyIle: 7.017 ± 0.093
4.713GlyLys: 4.713 ± 0.083
6.101GlyLeu: 6.101 ± 0.083
2.292GlyMet: 2.292 ± 0.048
3.072GlyAsn: 3.072 ± 0.07
2.282GlyPro: 2.282 ± 0.058
1.915GlyGln: 1.915 ± 0.048
3.246GlyArg: 3.246 ± 0.067
5.457GlySer: 5.457 ± 0.092
4.857GlyThr: 4.857 ± 0.077
5.348GlyVal: 5.348 ± 0.069
0.859GlyTrp: 0.859 ± 0.026
3.017GlyTyr: 3.017 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
1.428HisAla: 1.428 ± 0.039
0.301HisCys: 0.301 ± 0.018
1.248HisAsp: 1.248 ± 0.036
1.324HisGlu: 1.324 ± 0.038
0.713HisPhe: 0.713 ± 0.028
1.441HisGly: 1.441 ± 0.041
0.619HisHis: 0.619 ± 0.023
1.579HisIle: 1.579 ± 0.038
0.753HisLys: 0.753 ± 0.026
2.119HisLeu: 2.119 ± 0.049
0.439HisMet: 0.439 ± 0.025
0.736HisAsn: 0.736 ± 0.028
1.352HisPro: 1.352 ± 0.034
0.795HisGln: 0.795 ± 0.026
1.015HisArg: 1.015 ± 0.035
1.186HisSer: 1.186 ± 0.036
1.168HisThr: 1.168 ± 0.041
1.245HisVal: 1.245 ± 0.033
0.157HisTrp: 0.157 ± 0.012
0.637HisTyr: 0.637 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
6.125IleAla: 6.125 ± 0.08
1.274IleCys: 1.274 ± 0.038
4.462IleAsp: 4.462 ± 0.084
4.9IleGlu: 4.9 ± 0.08
3.39IlePhe: 3.39 ± 0.078
6.012IleGly: 6.012 ± 0.085
1.772IleHis: 1.772 ± 0.047
7.237IleIle: 7.237 ± 0.11
3.833IleLys: 3.833 ± 0.076
8.487IleLeu: 8.487 ± 0.121
1.7IleMet: 1.7 ± 0.045
3.376IleAsn: 3.376 ± 0.078
4.842IlePro: 4.842 ± 0.072
2.935IleGln: 2.935 ± 0.06
4.745IleArg: 4.745 ± 0.075
7.452IleSer: 7.452 ± 0.098
5.927IleThr: 5.927 ± 0.085
4.921IleVal: 4.921 ± 0.072
0.843IleTrp: 0.843 ± 0.031
2.15IleTyr: 2.15 ± 0.046
0.0IleXaa: 0.0 ± 0.0
Lys
3.431LysAla: 3.431 ± 0.067
0.483LysCys: 0.483 ± 0.022
2.865LysAsp: 2.865 ± 0.056
3.915LysGlu: 3.915 ± 0.072
1.589LysPhe: 1.589 ± 0.043
3.555LysGly: 3.555 ± 0.057
0.79LysHis: 0.79 ± 0.029
4.488LysIle: 4.488 ± 0.081
3.822LysLys: 3.822 ± 0.075
3.341LysLeu: 3.341 ± 0.053
1.393LysMet: 1.393 ± 0.041
2.876LysAsn: 2.876 ± 0.059
2.313LysPro: 2.313 ± 0.053
1.696LysGln: 1.696 ± 0.048
2.366LysArg: 2.366 ± 0.05
3.266LysSer: 3.266 ± 0.052
3.421LysThr: 3.421 ± 0.064
3.16LysVal: 3.16 ± 0.061
0.482LysTrp: 0.482 ± 0.025
1.768LysTyr: 1.768 ± 0.042
0.0LysXaa: 0.0 ± 0.0
Leu
6.379LeuAla: 6.379 ± 0.079
1.216LeuCys: 1.216 ± 0.039
4.809LeuAsp: 4.809 ± 0.077
5.069LeuGlu: 5.069 ± 0.076
4.325LeuPhe: 4.325 ± 0.079
5.789LeuGly: 5.789 ± 0.082
1.862LeuHis: 1.862 ± 0.043
8.442LeuIle: 8.442 ± 0.112
5.028LeuLys: 5.028 ± 0.073
8.888LeuLeu: 8.888 ± 0.125
2.31LeuMet: 2.31 ± 0.051
4.148LeuAsn: 4.148 ± 0.077
3.955LeuPro: 3.955 ± 0.065
2.799LeuGln: 2.799 ± 0.054
3.564LeuArg: 3.564 ± 0.067
7.62LeuSer: 7.62 ± 0.093
5.539LeuThr: 5.539 ± 0.069
6.257LeuVal: 6.257 ± 0.089
0.782LeuTrp: 0.782 ± 0.028
2.74LeuTyr: 2.74 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
1.929MetAla: 1.929 ± 0.045
0.224MetCys: 0.224 ± 0.015
1.49MetAsp: 1.49 ± 0.036
1.475MetGlu: 1.475 ± 0.042
0.76MetPhe: 0.76 ± 0.031
1.849MetGly: 1.849 ± 0.05
0.469MetHis: 0.469 ± 0.019
2.198MetIle: 2.198 ± 0.05
1.675MetLys: 1.675 ± 0.047
1.941MetLeu: 1.941 ± 0.045
0.762MetMet: 0.762 ± 0.032
1.449MetAsn: 1.449 ± 0.037
1.043MetPro: 1.043 ± 0.032
0.913MetGln: 0.913 ± 0.031
1.168MetArg: 1.168 ± 0.037
1.624MetSer: 1.624 ± 0.042
1.616MetThr: 1.616 ± 0.039
1.849MetVal: 1.849 ± 0.05
0.177MetTrp: 0.177 ± 0.017
0.693MetTyr: 0.693 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
2.824AsnAla: 2.824 ± 0.059
0.501AsnCys: 0.501 ± 0.024
1.799AsnAsp: 1.799 ± 0.043
2.426AsnGlu: 2.426 ± 0.055
1.397AsnPhe: 1.397 ± 0.038
2.988AsnGly: 2.988 ± 0.065
0.878AsnHis: 0.878 ± 0.03
3.301AsnIle: 3.301 ± 0.072
1.836AsnLys: 1.836 ± 0.048
4.367AsnLeu: 4.367 ± 0.076
0.899AsnMet: 0.899 ± 0.028
2.123AsnAsn: 2.123 ± 0.075
2.848AsnPro: 2.848 ± 0.061
1.888AsnGln: 1.888 ± 0.045
2.087AsnArg: 2.087 ± 0.049
2.901AsnSer: 2.901 ± 0.072
2.53AsnThr: 2.53 ± 0.06
2.409AsnVal: 2.409 ± 0.05
0.405AsnTrp: 0.405 ± 0.021
1.263AsnTyr: 1.263 ± 0.039
0.0AsnXaa: 0.0 ± 0.0
Pro
3.145ProAla: 3.145 ± 0.062
0.533ProCys: 0.533 ± 0.024
3.929ProAsp: 3.929 ± 0.058
3.491ProGlu: 3.491 ± 0.059
1.836ProPhe: 1.836 ± 0.044
3.889ProGly: 3.889 ± 0.078
0.866ProHis: 0.866 ± 0.029
3.076ProIle: 3.076 ± 0.058
1.675ProLys: 1.675 ± 0.041
3.909ProLeu: 3.909 ± 0.067
0.898ProMet: 0.898 ± 0.031
1.394ProAsn: 1.394 ± 0.038
1.714ProPro: 1.714 ± 0.047
1.145ProGln: 1.145 ± 0.031
1.51ProArg: 1.51 ± 0.04
2.997ProSer: 2.997 ± 0.064
2.343ProThr: 2.343 ± 0.078
4.582ProVal: 4.582 ± 0.074
0.448ProTrp: 0.448 ± 0.021
1.498ProTyr: 1.498 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
2.434GlnAla: 2.434 ± 0.052
0.316GlnCys: 0.316 ± 0.02
1.735GlnAsp: 1.735 ± 0.037
2.189GlnGlu: 2.189 ± 0.052
1.314GlnPhe: 1.314 ± 0.042
2.108GlnGly: 2.108 ± 0.045
0.538GlnHis: 0.538 ± 0.022
3.109GlnIle: 3.109 ± 0.054
2.269GlnLys: 2.269 ± 0.05
2.149GlnLeu: 2.149 ± 0.044
1.05GlnMet: 1.05 ± 0.03
1.715GlnAsn: 1.715 ± 0.044
1.104GlnPro: 1.104 ± 0.034
1.134GlnGln: 1.134 ± 0.039
1.339GlnArg: 1.339 ± 0.034
2.003GlnSer: 2.003 ± 0.046
2.035GlnThr: 2.035 ± 0.049
2.551GlnVal: 2.551 ± 0.05
0.311GlnTrp: 0.311 ± 0.018
1.359GlnTyr: 1.359 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
2.844ArgAla: 2.844 ± 0.061
0.695ArgCys: 0.695 ± 0.027
2.858ArgAsp: 2.858 ± 0.055
3.632ArgGlu: 3.632 ± 0.076
2.149ArgPhe: 2.149 ± 0.045
2.785ArgGly: 2.785 ± 0.053
0.948ArgHis: 0.948 ± 0.029
4.34ArgIle: 4.34 ± 0.081
2.615ArgLys: 2.615 ± 0.056
3.999ArgLeu: 3.999 ± 0.065
1.368ArgMet: 1.368 ± 0.035
1.949ArgAsn: 1.949 ± 0.052
1.54ArgPro: 1.54 ± 0.04
1.487ArgGln: 1.487 ± 0.042
2.392ArgArg: 2.392 ± 0.054
3.171ArgSer: 3.171 ± 0.067
2.565ArgThr: 2.565 ± 0.053
3.159ArgVal: 3.159 ± 0.049
0.524ArgTrp: 0.524 ± 0.025
2.058ArgTyr: 2.058 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
4.74SerAla: 4.74 ± 0.085
1.003SerCys: 1.003 ± 0.031
4.644SerAsp: 4.644 ± 0.072
4.425SerGlu: 4.425 ± 0.075
2.939SerPhe: 2.939 ± 0.058
6.803SerGly: 6.803 ± 0.104
1.665SerHis: 1.665 ± 0.041
5.611SerIle: 5.611 ± 0.083
2.76SerLys: 2.76 ± 0.067
6.911SerLeu: 6.911 ± 0.104
1.766SerMet: 1.766 ± 0.042
2.575SerAsn: 2.575 ± 0.06
3.336SerPro: 3.336 ± 0.066
2.29SerGln: 2.29 ± 0.053
3.568SerArg: 3.568 ± 0.06
5.99SerSer: 5.99 ± 0.116
3.887SerThr: 3.887 ± 0.073
5.003SerVal: 5.003 ± 0.072
0.814SerTrp: 0.814 ± 0.029
2.31SerTyr: 2.31 ± 0.046
0.0SerXaa: 0.0 ± 0.0
Thr
4.162ThrAla: 4.162 ± 0.064
0.767ThrCys: 0.767 ± 0.027
3.448ThrAsp: 3.448 ± 0.07
3.171ThrGlu: 3.171 ± 0.052
2.224ThrPhe: 2.224 ± 0.043
5.896ThrGly: 5.896 ± 0.085
1.167ThrHis: 1.167 ± 0.042
5.179ThrIle: 5.179 ± 0.078
2.466ThrLys: 2.466 ± 0.061
5.536ThrLeu: 5.536 ± 0.071
1.266ThrMet: 1.266 ± 0.037
2.261ThrAsn: 2.261 ± 0.052
3.389ThrPro: 3.389 ± 0.081
1.593ThrGln: 1.593 ± 0.042
2.739ThrArg: 2.739 ± 0.055
4.337ThrSer: 4.337 ± 0.078
3.513ThrThr: 3.513 ± 0.079
4.316ThrVal: 4.316 ± 0.09
0.686ThrTrp: 0.686 ± 0.027
1.947ThrTyr: 1.947 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
4.664ValAla: 4.664 ± 0.082
1.085ValCys: 1.085 ± 0.032
3.447ValAsp: 3.447 ± 0.06
3.598ValGlu: 3.598 ± 0.068
2.794ValPhe: 2.794 ± 0.053
4.119ValGly: 4.119 ± 0.074
1.411ValHis: 1.411 ± 0.034
6.166ValIle: 6.166 ± 0.075
3.424ValLys: 3.424 ± 0.066
6.225ValLeu: 6.225 ± 0.076
1.87ValMet: 1.87 ± 0.046
2.643ValAsn: 2.643 ± 0.068
3.184ValPro: 3.184 ± 0.066
2.177ValGln: 2.177 ± 0.051
3.41ValArg: 3.41 ± 0.072
5.587ValSer: 5.587 ± 0.078
4.766ValThr: 4.766 ± 0.074
4.84ValVal: 4.84 ± 0.089
0.672ValTrp: 0.672 ± 0.027
2.166ValTyr: 2.166 ± 0.051
0.0ValXaa: 0.0 ± 0.0
Trp
0.604TrpAla: 0.604 ± 0.028
0.103TrpCys: 0.103 ± 0.01
0.632TrpAsp: 0.632 ± 0.031
0.546TrpGlu: 0.546 ± 0.024
0.481TrpPhe: 0.481 ± 0.024
0.599TrpGly: 0.599 ± 0.025
0.195TrpHis: 0.195 ± 0.011
0.976TrpIle: 0.976 ± 0.032
0.674TrpLys: 0.674 ± 0.028
0.827TrpLeu: 0.827 ± 0.033
0.303TrpMet: 0.303 ± 0.019
0.758TrpAsn: 0.758 ± 0.03
0.266TrpPro: 0.266 ± 0.016
0.392TrpGln: 0.392 ± 0.025
0.501TrpArg: 0.501 ± 0.023
0.609TrpSer: 0.609 ± 0.025
0.528TrpThr: 0.528 ± 0.022
0.671TrpVal: 0.671 ± 0.023
0.169TrpTrp: 0.169 ± 0.013
0.468TrpTyr: 0.468 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.344TyrAla: 2.344 ± 0.048
0.467TyrCys: 0.467 ± 0.021
1.868TyrAsp: 1.868 ± 0.042
1.873TyrGlu: 1.873 ± 0.052
1.244TyrPhe: 1.244 ± 0.037
2.397TyrGly: 2.397 ± 0.056
0.832TyrHis: 0.832 ± 0.031
2.28TyrIle: 2.28 ± 0.047
1.22TyrLys: 1.22 ± 0.033
3.592TyrLeu: 3.592 ± 0.061
0.664TyrMet: 0.664 ± 0.027
1.408TyrAsn: 1.408 ± 0.038
1.688TyrPro: 1.688 ± 0.041
1.527TyrGln: 1.527 ± 0.039
1.742TyrArg: 1.742 ± 0.043
2.441TyrSer: 2.441 ± 0.056
2.009TyrThr: 2.009 ± 0.046
1.787TyrVal: 1.787 ± 0.044
0.377TyrTrp: 0.377 ± 0.021
1.262TyrTyr: 1.262 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3377 proteins (1080887 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski