Pseudomonas phage PRR1 (Bacteriophage PRR1)

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Leviviricetes; Norzivirales; Fiersviridae; Perrunavirus; Perrunavirus olsenii

Average proteome isoelectric point is 8.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0PH28|Q0PH28_BPPRR Maturation protein OS=Pseudomonas phage PRR1 OX=12024 PE=4 SV=1
MM1 pKa = 7.48AQLQNLVLKK10 pKa = 10.67DD11 pKa = 4.12RR12 pKa = 11.84EE13 pKa = 4.46ATPNDD18 pKa = 3.45HH19 pKa = 6.49TFVPRR24 pKa = 11.84DD25 pKa = 3.08IRR27 pKa = 11.84DD28 pKa = 3.51NVGEE32 pKa = 4.28VVEE35 pKa = 4.52STGVPIGEE43 pKa = 4.2SRR45 pKa = 11.84FTISLRR51 pKa = 11.84KK52 pKa = 7.92TSNGRR57 pKa = 11.84YY58 pKa = 9.42KK59 pKa = 10.05STLKK63 pKa = 10.84LVVPVVQSQTVNGIVTPVVVRR84 pKa = 11.84TSYY87 pKa = 9.28VTIDD91 pKa = 3.53FDD93 pKa = 4.16YY94 pKa = 11.1DD95 pKa = 3.25ARR97 pKa = 11.84STTKK101 pKa = 10.06EE102 pKa = 3.69RR103 pKa = 11.84NNFVGMIADD112 pKa = 3.8ALKK115 pKa = 9.83ADD117 pKa = 3.69KK118 pKa = 10.4MLVHH122 pKa = 6.38DD123 pKa = 5.3TIVNLQGVYY132 pKa = 10.29

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0PH27|Q0PH27_BPPRR Coat protein OS=Pseudomonas phage PRR1 OX=12024 PE=4 SV=1
MM1 pKa = 7.55CKK3 pKa = 10.37VSTKK7 pKa = 10.14VDD9 pKa = 3.41SKK11 pKa = 10.25LTEE14 pKa = 3.99SVGQLTIRR22 pKa = 11.84SYY24 pKa = 11.19LWLRR28 pKa = 11.84NILALAGLLFVILLATNHH46 pKa = 6.19LSIAIYY52 pKa = 10.54SPP54 pKa = 3.4

Molecular weight: 5.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1143

54

540

285.8

32.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.512 ± 0.328

0.962 ± 0.439

5.424 ± 0.776

4.374 ± 0.437

4.287 ± 0.534

6.999 ± 0.677

1.487 ± 0.05

5.249 ± 0.519

4.899 ± 0.182

9.974 ± 1.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.273

4.637 ± 0.383

4.987 ± 0.655

2.1 ± 0.377

7.962 ± 0.717

9.099 ± 0.558

6.474 ± 1.033

7.524 ± 1.861

2.1 ± 0.466

3.85 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski