Proteome-pI 2.0 - Proteome Isoelectric Point Database

Carnobacterium divergens DSM 20623

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae; Carnobacterium; Carnobacterium divergens

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 2398 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A0R2HPM2|A0A0R2HPM2_CARDV Uncharacterized protein OS=Carnobacterium divergens DSM 20623 OX=1449336 GN=IV74_GL002397 PE=4 SV=1

MM1 pKa = 6.96THH3 pKa = 6.7DD4 pKa = 4.16HH5 pKa = 5.71NHH7 pKa = 6.34EE8 pKa = 4.26EE9 pKa = 4.0DD10 pKa = 3.69HH11 pKa = 6.99HH12 pKa = 7.61DD13 pKa = 3.97HH14 pKa = 5.86EE15 pKa = 6.46HH16 pKa = 5.6ITIIDD21 pKa = 3.61EE22 pKa = 4.39NGNEE26 pKa = 4.05EE27 pKa = 4.39LYY29 pKa = 10.83EE30 pKa = 3.88ILFTFDD36 pKa = 2.93SDD38 pKa = 5.21DD39 pKa = 3.61YY40 pKa = 10.94AKK42 pKa = 10.64SYY44 pKa = 10.36VLVYY48 pKa = 9.66PAGIPEE54 pKa = 4.27GEE56 pKa = 4.32EE57 pKa = 3.73IEE59 pKa = 4.44LQAFSYY65 pKa = 10.92VEE67 pKa = 3.98QEE69 pKa = 4.94DD70 pKa = 4.61GNQGDD75 pKa = 4.79LKK77 pKa = 10.72PIEE80 pKa = 4.53TNEE83 pKa = 3.76EE84 pKa = 3.18WDD86 pKa = 3.71MIEE89 pKa = 4.21EE90 pKa = 4.12VLNTFMADD98 pKa = 3.7DD99 pKa = 3.96EE100 pKa = 4.7EE101 pKa = 4.55EE102 pKa = 3.97

Molecular weight: 11.99 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A0R2HXL9|A0A0R2HXL9_CARDV GntR family transcriptional regulator OS=Carnobacterium divergens DSM 20623 OX=1449336 GN=IV74_GL000023 PE=4 SV=1

MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.19NGRR28 pKa = 11.84RR29 pKa = 11.84VLQSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.98GRR39 pKa = 11.84NVLSAA44 pKa = 3.9

Molecular weight: 5.41 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
2398	0	2398	753840	44	3404	314.4	35.19

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
6.979 ± 0.047	0.56 ± 0.013	5.136 ± 0.042	7.362 ± 0.063	4.53 ± 0.042	6.597 ± 0.051	1.751 ± 0.02	7.898 ± 0.046	7.432 ± 0.047	9.896 ± 0.054

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.701 ± 0.025	4.871 ± 0.041	3.419 ± 0.025	3.922 ± 0.033	3.316 ± 0.037	6.101 ± 0.044	6.208 ± 0.046	6.93 ± 0.04	0.861 ± 0.017	3.525 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski