Lake Sarah-associated circular virus-37

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQP5|A0A140AQP5_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-37 OX=1685765 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.79SFRR5 pKa = 11.84QTKK8 pKa = 7.3GTWWSVTAYY17 pKa = 9.99GDD19 pKa = 4.08NIIKK23 pKa = 10.75LEE25 pKa = 4.58DD26 pKa = 3.38KK27 pKa = 10.93DD28 pKa = 5.47AIPDD32 pKa = 3.92YY33 pKa = 10.96IRR35 pKa = 11.84TIYY38 pKa = 10.17GGRR41 pKa = 11.84EE42 pKa = 3.66QCPTTGRR49 pKa = 11.84LHH51 pKa = 5.97FQGALQCASQCRR63 pKa = 11.84AGRR66 pKa = 11.84ILDD69 pKa = 3.71WLPGVHH75 pKa = 6.92LEE77 pKa = 4.29KK78 pKa = 10.77AKK80 pKa = 10.82NSHH83 pKa = 5.9ALKK86 pKa = 10.09KK87 pKa = 10.52YY88 pKa = 9.23VMKK91 pKa = 10.75EE92 pKa = 3.41EE93 pKa = 3.94TAAGEE98 pKa = 4.03KK99 pKa = 10.12TEE101 pKa = 4.28VINKK105 pKa = 9.46NEE107 pKa = 3.84YY108 pKa = 7.15TTLQKK113 pKa = 11.14AMEE116 pKa = 4.53LLAEE120 pKa = 4.19EE121 pKa = 5.4NIVQTDD127 pKa = 3.11IQTDD131 pKa = 3.46KK132 pKa = 10.72LTAKK136 pKa = 10.41QIASHH141 pKa = 5.88QYY143 pKa = 6.35WQRR146 pKa = 11.84VRR148 pKa = 11.84QVLEE152 pKa = 3.78KK153 pKa = 10.52RR154 pKa = 11.84PEE156 pKa = 3.96LVSIYY161 pKa = 10.6ASPLAKK167 pKa = 9.54TAWTNTCSVWMKK179 pKa = 10.31KK180 pKa = 9.46RR181 pKa = 11.84AIVLQPASEE190 pKa = 4.09EE191 pKa = 4.31FISGEE196 pKa = 4.19EE197 pKa = 3.89KK198 pKa = 10.9SLL200 pKa = 3.65

Molecular weight: 22.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQP5|A0A140AQP5_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-37 OX=1685765 PE=4 SV=1
MM1 pKa = 7.66AFIPKK6 pKa = 9.8RR7 pKa = 11.84KK8 pKa = 9.0YY9 pKa = 9.85ASKK12 pKa = 10.67RR13 pKa = 11.84YY14 pKa = 9.27AKK16 pKa = 10.25KK17 pKa = 10.4SAIKK21 pKa = 10.38RR22 pKa = 11.84MASGPSSQIKK32 pKa = 8.3TLAKK36 pKa = 10.05AVRR39 pKa = 11.84TLQKK43 pKa = 10.22KK44 pKa = 10.43DD45 pKa = 3.35KK46 pKa = 10.8TDD48 pKa = 3.13AQYY51 pKa = 11.79LNFTQQVFEE60 pKa = 4.66QNVQSPVYY68 pKa = 9.11TVNLSDD74 pKa = 3.84YY75 pKa = 10.59SGMISTFGTSANDD88 pKa = 3.37NVDD91 pKa = 2.57NRR93 pKa = 11.84IIHH96 pKa = 6.37KK97 pKa = 10.19SVGMDD102 pKa = 3.28CRR104 pKa = 11.84VTLEE108 pKa = 3.7NAINNEE114 pKa = 3.62EE115 pKa = 4.16STIGFTAFLVSLKK128 pKa = 10.75DD129 pKa = 3.55AVGNVYY135 pKa = 11.14NNLTGGLTLTLGATHH150 pKa = 6.64EE151 pKa = 4.62MIQGMVLLNKK161 pKa = 10.2QIFNIHH167 pKa = 5.0KK168 pKa = 9.72VKK170 pKa = 10.78RR171 pKa = 11.84FTLTNYY177 pKa = 9.32NQALISPAAQSQYY190 pKa = 9.91GTDD193 pKa = 3.44HH194 pKa = 6.36RR195 pKa = 11.84WYY197 pKa = 9.55WRR199 pKa = 11.84LPINKK204 pKa = 8.79TIVNPRR210 pKa = 11.84GDD212 pKa = 3.64WKK214 pKa = 11.11QLGCALDD221 pKa = 3.9PSKK224 pKa = 10.87QYY226 pKa = 11.9YY227 pKa = 11.05LMIFNDD233 pKa = 3.5NSTSDD238 pKa = 3.8LEE240 pKa = 4.4SPAFSFVNVHH250 pKa = 5.25TFKK253 pKa = 10.7TLGG256 pKa = 3.36

Molecular weight: 28.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

456

200

256

228.0

25.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.895 ± 0.685

1.316 ± 0.424

4.167 ± 0.413

5.263 ± 2.315

3.289 ± 1.109

5.263 ± 0.163

1.974 ± 0.016

6.14 ± 0.223

8.333 ± 0.413

8.114 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.255

5.921 ± 1.809

3.07 ± 0.043

5.921 ± 0.359

4.386 ± 0.38

6.798 ± 0.804

8.114 ± 0.071

5.702 ± 0.435

1.974 ± 0.636

3.947 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski