Pseudomonas phage PaMx42

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Septimatrevirus; unclassified Septimatrevirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S0MV37|A0A0S0MV37_9CAUD Uncharacterized protein OS=Pseudomonas phage PaMx42 OX=1175662 GN=PaMx42_46 PE=4 SV=1
MM1 pKa = 7.36GAYY4 pKa = 8.0TVTTEE9 pKa = 4.8FEE11 pKa = 4.38MNDD14 pKa = 3.18GRR16 pKa = 11.84ILSCEE21 pKa = 4.05YY22 pKa = 10.69GVSFTPGNYY31 pKa = 9.59SGLPEE36 pKa = 3.95NCYY39 pKa = 9.96PDD41 pKa = 3.68EE42 pKa = 5.41SEE44 pKa = 4.73AGEE47 pKa = 3.85PTYY50 pKa = 10.98YY51 pKa = 9.7IDD53 pKa = 5.4GEE55 pKa = 4.3EE56 pKa = 3.99VDD58 pKa = 5.38YY59 pKa = 11.6KK60 pKa = 11.02DD61 pKa = 5.12LPKK64 pKa = 11.02GLDD67 pKa = 3.34KK68 pKa = 10.99IADD71 pKa = 3.8KK72 pKa = 10.79LYY74 pKa = 9.4EE75 pKa = 4.62AGPGEE80 pKa = 4.26YY81 pKa = 9.83GYY83 pKa = 10.97SEE85 pKa = 4.61TEE87 pKa = 3.67PDD89 pKa = 3.47YY90 pKa = 11.36DD91 pKa = 4.2GPDD94 pKa = 3.71YY95 pKa = 11.4EE96 pKa = 5.96PDD98 pKa = 3.39DD99 pKa = 4.41YY100 pKa = 11.83YY101 pKa = 11.96

Molecular weight: 11.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S0MWU0|A0A0S0MWU0_9CAUD Virion structural protein OS=Pseudomonas phage PaMx42 OX=1175662 GN=PaMx42_23 PE=4 SV=1
MM1 pKa = 7.37NKK3 pKa = 9.02QRR5 pKa = 11.84PFGAIFQGVQQMFAHH20 pKa = 6.2YY21 pKa = 8.92FASKK25 pKa = 8.23KK26 pKa = 8.8TKK28 pKa = 9.75FVVISEE34 pKa = 4.43TNRR37 pKa = 11.84PEE39 pKa = 4.06GQRR42 pKa = 11.84IAVSGKK48 pKa = 9.12AEE50 pKa = 3.73ARR52 pKa = 11.84RR53 pKa = 11.84VAKK56 pKa = 9.88EE57 pKa = 3.85RR58 pKa = 11.84NAQPWNFF65 pKa = 3.44

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13720

57

1275

245.0

26.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.385 ± 0.865

1.05 ± 0.166

5.204 ± 0.263

6.159 ± 0.346

3.79 ± 0.189

7.748 ± 0.25

1.334 ± 0.169

5.313 ± 0.177

5.554 ± 0.322

7.566 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.23 ± 0.125

4.708 ± 0.24

5.109 ± 0.425

4.3 ± 0.628

5.204 ± 0.238

5.62 ± 0.255

6.188 ± 0.364

6.873 ± 0.252

1.378 ± 0.133

3.287 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski