Escherichia phage vB_EcoS_PHB17

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Changchunvirus; Escherichia virus PHB17

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DKT0|A0A514DKT0_9CAUD Putative spanin OS=Escherichia phage vB_EcoS_PHB17 OX=2591407 PE=4 SV=1
MM1 pKa = 6.83VQDD4 pKa = 3.42EE5 pKa = 4.76FFARR9 pKa = 11.84LHH11 pKa = 5.41EE12 pKa = 4.57AEE14 pKa = 4.21SAGLNKK20 pKa = 9.58EE21 pKa = 3.8AALEE25 pKa = 4.0VAYY28 pKa = 9.88KK29 pKa = 10.64LISLEE34 pKa = 4.19DD35 pKa = 3.5ALGAMDD41 pKa = 4.65MDD43 pKa = 4.17QEE45 pKa = 4.38SGAIFADD52 pKa = 3.5PTTIINDD59 pKa = 3.92CGCNFDD65 pKa = 4.8PSCKK69 pKa = 9.76RR70 pKa = 11.84CFPFF74 pKa = 5.75

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DKM4|A0A514DKM4_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_PHB17 OX=2591407 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.55KK3 pKa = 10.36EE4 pKa = 3.74ILKK7 pKa = 10.32LGKK10 pKa = 10.07GSVMRR15 pKa = 11.84CINSDD20 pKa = 3.8LNCFKK25 pKa = 11.0VGEE28 pKa = 4.12NYY30 pKa = 8.55ITGKK34 pKa = 10.36RR35 pKa = 11.84LFIGNRR41 pKa = 11.84HH42 pKa = 5.37IGIGRR47 pKa = 11.84FIVNHH52 pKa = 6.24HH53 pKa = 6.21KK54 pKa = 10.69NGYY57 pKa = 9.63FGIEE61 pKa = 4.27DD62 pKa = 3.91LSGVIARR69 pKa = 11.84FVLVKK74 pKa = 10.54DD75 pKa = 3.55SKK77 pKa = 11.28GRR79 pKa = 11.84YY80 pKa = 8.34AKK82 pKa = 10.37HH83 pKa = 5.52DD84 pKa = 3.84TKK86 pKa = 11.04PNPHH90 pKa = 6.89KK91 pKa = 10.52DD92 pKa = 3.09RR93 pKa = 11.84RR94 pKa = 11.84NGKK97 pKa = 8.72RR98 pKa = 11.84DD99 pKa = 2.89ARR101 pKa = 11.84RR102 pKa = 11.84FRR104 pKa = 11.84RR105 pKa = 11.84YY106 pKa = 9.09ARR108 pKa = 11.84QMLKK112 pKa = 10.09CDD114 pKa = 3.27KK115 pKa = 10.53PGTLEE120 pKa = 3.91YY121 pKa = 10.82KK122 pKa = 10.55VISNLARR129 pKa = 11.84NVGKK133 pKa = 10.43

Molecular weight: 15.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

14973

33

1186

176.2

19.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.589 ± 0.522

1.369 ± 0.215

5.824 ± 0.209

6.973 ± 0.265

4.101 ± 0.23

7.4 ± 0.225

1.863 ± 0.211

6.131 ± 0.213

7.286 ± 0.362

6.879 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.899 ± 0.195

4.702 ± 0.22

3.159 ± 0.222

3.867 ± 0.277

5.149 ± 0.208

6.198 ± 0.293

5.483 ± 0.267

7.126 ± 0.267

1.496 ± 0.129

3.506 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski