Aeromonas phage D3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514TVI8|A0A514TVI8_9CAUD Uncharacterized protein OS=Aeromonas phage D3 OX=2593327 GN=D3_0044 PE=4 SV=1
MM1 pKa = 7.61CGSDD5 pKa = 3.66TPFVLQDD12 pKa = 3.84TIVHH16 pKa = 6.83LDD18 pKa = 3.65LLIDD22 pKa = 3.74EE23 pKa = 4.81VDD25 pKa = 3.5RR26 pKa = 11.84SKK28 pKa = 11.67DD29 pKa = 3.13LANFYY34 pKa = 11.14FEE36 pKa = 4.86GNIFIIVEE44 pKa = 4.12DD45 pKa = 4.26PYY47 pKa = 11.4GTVV50 pKa = 2.95

Molecular weight: 5.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514TVK5|A0A514TVK5_9CAUD Uncharacterized protein OS=Aeromonas phage D3 OX=2593327 GN=D3_0058 PE=4 SV=1
MM1 pKa = 7.34FEE3 pKa = 5.02AMSNSQAFGFAFFGAIGVIAVIAIGANEE31 pKa = 3.83YY32 pKa = 10.62FKK34 pKa = 11.16AKK36 pKa = 10.0KK37 pKa = 9.68ALKK40 pKa = 10.21RR41 pKa = 11.84PMATRR46 pKa = 11.84NKK48 pKa = 10.12RR49 pKa = 3.23

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

267

0

267

82310

30

2794

308.3

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.488 ± 0.156

0.914 ± 0.051

6.386 ± 0.091

6.694 ± 0.17

4.615 ± 0.117

6.824 ± 0.141

2.023 ± 0.079

6.296 ± 0.144

6.275 ± 0.124

8.178 ± 0.172

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.201 ± 0.094

5.437 ± 0.153

4.047 ± 0.125

3.303 ± 0.1

4.775 ± 0.116

5.988 ± 0.101

6.333 ± 0.203

7.183 ± 0.157

1.189 ± 0.072

3.851 ± 0.098
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski