Aquaspirillum sp. LM1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Chromobacteriaceae; Aquaspirillum; unclassified Aquaspirillum

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3268 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1U9JLK1|A0A1U9JLK1_9NEIS Uncharacterized protein OS=Aquaspirillum sp. LM1 OX=1938604 GN=BXU06_07570 PE=4 SV=1
MM1 pKa = 7.89DD2 pKa = 4.9KK3 pKa = 10.82SYY5 pKa = 10.0TCILIADD12 pKa = 4.35ATLQDD17 pKa = 4.69LPVLLADD24 pKa = 4.9LPASVHH30 pKa = 5.62LRR32 pKa = 11.84LVHH35 pKa = 6.23SASEE39 pKa = 3.92ALGEE43 pKa = 4.26FWQALASAGLTQLHH57 pKa = 6.25WLSHH61 pKa = 4.51GMPGGVMIAGQALTAADD78 pKa = 4.42FCQRR82 pKa = 11.84FDD84 pKa = 3.47GAAEE88 pKa = 4.06RR89 pKa = 11.84DD90 pKa = 3.42LDD92 pKa = 3.85VAFWSCHH99 pKa = 4.97TGAGEE104 pKa = 3.6PGYY107 pKa = 11.16AFVEE111 pKa = 4.18ALTRR115 pKa = 11.84ATGAQVAASAVEE127 pKa = 4.15VGAGFWQLSPAVNAPFSARR146 pKa = 11.84AQLEE150 pKa = 4.28FAHH153 pKa = 6.24TLPAPAQFASSVTYY167 pKa = 10.17QASQEE172 pKa = 4.11MGGLALGDD180 pKa = 3.95LNGDD184 pKa = 3.59GTLDD188 pKa = 3.29IQTTGIFNDD197 pKa = 3.55ASTLINDD204 pKa = 4.07GDD206 pKa = 3.93GSFANDD212 pKa = 3.51ASGTGQPNGEE222 pKa = 4.41WGCEE226 pKa = 3.65LADD229 pKa = 4.58LNGDD233 pKa = 3.53GHH235 pKa = 7.77LDD237 pKa = 3.55LVSRR241 pKa = 11.84IYY243 pKa = 10.91NAVVVNRR250 pKa = 11.84GSAQNGFTNQQYY262 pKa = 9.78YY263 pKa = 9.46LQNEE267 pKa = 4.36TVYY270 pKa = 10.94DD271 pKa = 4.89AIAAQVNNDD280 pKa = 2.78GRR282 pKa = 11.84FDD284 pKa = 4.38LLVSRR289 pKa = 11.84TIQGQQTLSVLLGQSDD305 pKa = 4.12GTLAAPVNYY314 pKa = 10.24ALASEE319 pKa = 4.67GDD321 pKa = 3.83ANQLHH326 pKa = 5.95ATDD329 pKa = 5.09LNNDD333 pKa = 3.62GKK335 pKa = 10.9QDD337 pKa = 4.13VIALSYY343 pKa = 11.37NNTISVLMNNGDD355 pKa = 3.68GTFANSVSYY364 pKa = 10.86QEE366 pKa = 5.42GDD368 pKa = 3.58YY369 pKa = 11.12QNQLFNLTSADD380 pKa = 3.76VNGDD384 pKa = 3.7GYY386 pKa = 11.33QDD388 pKa = 3.53VVAVDD393 pKa = 3.47IFAGSVSVKK402 pKa = 10.32LNQGNGLLGPASHH415 pKa = 6.83YY416 pKa = 9.74ATGTAPTDD424 pKa = 3.36IVAVDD429 pKa = 4.15LNGDD433 pKa = 3.84GAVDD437 pKa = 4.22LATANTSSVSVLLGNGDD454 pKa = 3.45GSFAAKK460 pKa = 9.89QDD462 pKa = 3.45IQLDD466 pKa = 4.02TLPKK470 pKa = 9.91HH471 pKa = 6.26IRR473 pKa = 11.84AGDD476 pKa = 3.66LNQDD480 pKa = 3.62GKK482 pKa = 11.14QDD484 pKa = 3.95LVVGSYY490 pKa = 10.26SGNSVSVLLNTTGSVDD506 pKa = 3.65SIAPVVSRR514 pKa = 11.84LEE516 pKa = 3.94VAGTSLIVHH525 pKa = 5.36YY526 pKa = 8.96TEE528 pKa = 3.94ITQLDD533 pKa = 4.26AVHH536 pKa = 6.95PPALSQFAAKK546 pKa = 10.11INNSPVALTGAPVVDD561 pKa = 4.52AVHH564 pKa = 5.88HH565 pKa = 5.74TVTLTLGTPVALGQTVSLSYY585 pKa = 11.04TDD587 pKa = 4.8ASTNNDD593 pKa = 2.93VQAVQDD599 pKa = 3.98LAGNDD604 pKa = 3.43AASFTLTTAPMAAVRR619 pKa = 11.84FSADD623 pKa = 2.98TGVSDD628 pKa = 4.41SDD630 pKa = 5.29LITFQPVQTLSGHH643 pKa = 5.97LQTPTVDD650 pKa = 3.37GQTVQVSVNNGMSWEE665 pKa = 4.3TANHH669 pKa = 5.72STGQTYY675 pKa = 9.06WSLDD679 pKa = 3.34QSLLGQSLYY688 pKa = 10.61QGPNVLKK695 pKa = 10.88VRR697 pKa = 11.84LHH699 pKa = 5.48TASSNTEE706 pKa = 3.98VASQNYY712 pKa = 9.52DD713 pKa = 3.57LTWSGRR719 pKa = 11.84IFMEE723 pKa = 4.25SGSVNGNNIKK733 pKa = 10.24VQFSSDD739 pKa = 3.52LPLDD743 pKa = 4.05ASHH746 pKa = 7.36PPSLDD751 pKa = 3.34SFEE754 pKa = 4.67VLADD758 pKa = 3.73NVAVALSGVAVVDD771 pKa = 4.4GNARR775 pKa = 11.84TLTLTLANPVLAGQQVLVKK794 pKa = 10.52YY795 pKa = 9.71NDD797 pKa = 3.45PMPDD801 pKa = 3.47DD802 pKa = 4.15GQFILDD808 pKa = 4.92AMDD811 pKa = 4.5ALQDD815 pKa = 3.46IGGADD820 pKa = 3.75AMDD823 pKa = 3.68GMAMLSNYY831 pKa = 9.05TPDD834 pKa = 2.32ITAPVFRR841 pKa = 11.84SASINGSKK849 pKa = 10.49LVLSYY854 pKa = 11.7ADD856 pKa = 3.95ASGLDD861 pKa = 3.98VNNSPLLSAFSVKK874 pKa = 9.9VDD876 pKa = 3.51NSVVALTGTPVVNSGGKK893 pKa = 8.43TITLTLVNSVLNGQAVTVSYY913 pKa = 10.84TDD915 pKa = 3.44PTSGNDD921 pKa = 3.02SKK923 pKa = 11.41AIQDD927 pKa = 3.68VYY929 pKa = 11.69GNDD932 pKa = 3.65AASLAQVTVNNLTPIDD948 pKa = 3.69ITPPVLTSATINGKK962 pKa = 9.59KK963 pKa = 10.06LVLSYY968 pKa = 11.65ADD970 pKa = 3.82ASALHH975 pKa = 5.77SQIPLLSAFSVNVAGNAVLLSGGVPEE1001 pKa = 4.84VDD1003 pKa = 3.25TAAKK1007 pKa = 9.28TVTLTLINPVLNGQVVTVSYY1027 pKa = 10.81TDD1029 pKa = 3.48PTSGNDD1035 pKa = 3.1SQAIQDD1041 pKa = 3.76VYY1043 pKa = 11.71GNDD1046 pKa = 3.39AASLNNVAVSNITPVDD1062 pKa = 3.43TTPPVLTSATVSGHH1076 pKa = 5.21EE1077 pKa = 4.31VVLSYY1082 pKa = 11.34TDD1084 pKa = 3.63TNVLSSQGPLLSAFSVKK1101 pKa = 10.34LAGSVATLSGAPVVNTTAKK1120 pKa = 10.19TITLTLATPAQNGQAVTLSYY1140 pKa = 11.02TDD1142 pKa = 3.56PTSGNDD1148 pKa = 3.14SQAIQDD1154 pKa = 3.63ASGNDD1159 pKa = 3.35AASLNNIAVSNLTPLGDD1176 pKa = 3.48VTPPVLTSATVNGDD1190 pKa = 3.5QVLLSYY1196 pKa = 10.56TDD1198 pKa = 3.66SSPLKK1203 pKa = 10.27SSMGGLYY1210 pKa = 9.95VIKK1213 pKa = 10.54VDD1215 pKa = 3.6GVVAQTYY1222 pKa = 10.87GMTANPSNNTILITLANAVLAGQVVTFSYY1251 pKa = 10.98TDD1253 pKa = 3.46PTSGNDD1259 pKa = 3.35LLAIQDD1265 pKa = 3.69VAGNDD1270 pKa = 3.42AASVVDD1276 pKa = 5.07FSVSNLTVPTYY1287 pKa = 11.29QMAAGEE1293 pKa = 4.13TSYY1296 pKa = 10.71TVTATQYY1303 pKa = 11.43NVMGNALDD1311 pKa = 3.86NLMTGDD1317 pKa = 4.02SKK1319 pKa = 11.52PNNLMGGAGNDD1330 pKa = 3.56TLLGGGNNGDD1340 pKa = 3.59RR1341 pKa = 11.84LYY1343 pKa = 11.44GEE1345 pKa = 5.05AGNDD1349 pKa = 3.52VLNSGLGGSHH1359 pKa = 6.73RR1360 pKa = 11.84LVGGSGDD1367 pKa = 3.66DD1368 pKa = 3.33RR1369 pKa = 11.84YY1370 pKa = 10.92EE1371 pKa = 3.74IAYY1374 pKa = 9.78LATLLVEE1381 pKa = 5.17QPDD1384 pKa = 3.75EE1385 pKa = 4.95GNDD1388 pKa = 3.39SVYY1391 pKa = 11.33VMPGANLVVSLITLSANIEE1410 pKa = 3.86NLIAKK1415 pKa = 9.86NSEE1418 pKa = 3.89SFNVVGNASDD1428 pKa = 4.04NYY1430 pKa = 11.17LLGNQASGLLKK1441 pKa = 10.68GLAGNDD1447 pKa = 3.52TLISQGGDD1455 pKa = 3.19DD1456 pKa = 4.1TLEE1459 pKa = 4.65GGDD1462 pKa = 4.22GDD1464 pKa = 5.64DD1465 pKa = 4.12VIAPVAHH1472 pKa = 6.13SHH1474 pKa = 4.35MTEE1477 pKa = 3.65NTRR1480 pKa = 11.84VLTGNMQLSGGNGADD1495 pKa = 2.76IFVMAEE1501 pKa = 4.08GFVGDD1506 pKa = 5.78HH1507 pKa = 6.62KK1508 pKa = 11.35DD1509 pKa = 3.0GWNPNEE1515 pKa = 3.99FSGNYY1520 pKa = 8.45YY1521 pKa = 10.66SPSQPVGIQGVYY1533 pKa = 10.02ISDD1536 pKa = 4.07FEE1538 pKa = 4.76RR1539 pKa = 11.84GVDD1542 pKa = 3.98KK1543 pKa = 11.01ISLTLSEE1550 pKa = 4.55AALVPTTVNTLTLTAGMTLASAVDD1574 pKa = 3.75QAASRR1579 pKa = 11.84GSAAAPQVTIILNEE1593 pKa = 4.13NVGCAYY1599 pKa = 10.7LVLDD1603 pKa = 4.33QSDD1606 pKa = 3.61ASTFSSNDD1614 pKa = 3.09LAIKK1618 pKa = 10.38LNYY1621 pKa = 10.27SMPLGIGDD1629 pKa = 4.72LMFTKK1634 pKa = 10.78LSQQNQLLPAEE1645 pKa = 4.33SAPPVFTQASVNGNQLVLSYY1665 pKa = 11.3SDD1667 pKa = 4.79ASPLDD1672 pKa = 4.04SVALPLGAFAVMVNGNAASVSAASVNSLARR1702 pKa = 11.84TVTLTLASPVQNGQAVTVSYY1722 pKa = 10.58TDD1724 pKa = 3.62PSSGNDD1730 pKa = 2.9SAAIQDD1736 pKa = 3.93RR1737 pKa = 11.84YY1738 pKa = 11.38GNDD1741 pKa = 3.34ATSLAATPVNNLTTPADD1758 pKa = 3.46VTPPVLQSASLTDD1771 pKa = 3.43STLVLTYY1778 pKa = 10.86SDD1780 pKa = 4.25TSPLDD1785 pKa = 3.64AAHH1788 pKa = 7.11PPALSAFNVKK1798 pKa = 9.58VAGVTVSLTGTPVVNATARR1817 pKa = 11.84TVTLTLADD1825 pKa = 3.88PVLAGQAVTVSYY1837 pKa = 10.58TDD1839 pKa = 3.64PSSGNDD1845 pKa = 3.2AQAVQDD1851 pKa = 3.95GSGNDD1856 pKa = 3.33AQTVLNRR1863 pKa = 11.84VVSNLGTNTSYY1874 pKa = 11.92VMDD1877 pKa = 4.22ATTVNYY1883 pKa = 7.68TATGTAALTIQGNALNNQITGNAADD1908 pKa = 3.63NVIRR1912 pKa = 11.84GGQGNDD1918 pKa = 2.95RR1919 pKa = 11.84LLGGLGNDD1927 pKa = 3.4QLLGEE1932 pKa = 5.36DD1933 pKa = 4.88GSDD1936 pKa = 3.82SLDD1939 pKa = 3.51GGAGSDD1945 pKa = 3.84TLSGGAGNDD1954 pKa = 3.14AYY1956 pKa = 11.36YY1957 pKa = 10.84VDD1959 pKa = 5.13SVDD1962 pKa = 4.55DD1963 pKa = 4.34QIIEE1967 pKa = 4.03QANDD1971 pKa = 3.54SIADD1975 pKa = 3.68TVYY1978 pKa = 11.43ASINYY1983 pKa = 8.31TLGNDD1988 pKa = 3.79LEE1990 pKa = 4.68HH1991 pKa = 6.64LVLMGQATQGQGNEE2005 pKa = 4.16LANGLTGNALANTLTGLAGDD2025 pKa = 4.51DD2026 pKa = 4.8RR2027 pKa = 11.84IDD2029 pKa = 3.71GKK2031 pKa = 10.93QGADD2035 pKa = 3.02TMLGGTGNDD2044 pKa = 3.52YY2045 pKa = 11.24YY2046 pKa = 11.61YY2047 pKa = 11.12VDD2049 pKa = 3.48QAGDD2053 pKa = 3.89SVIEE2057 pKa = 4.2LANEE2061 pKa = 4.25GNDD2064 pKa = 3.59TIYY2067 pKa = 10.86AYY2069 pKa = 11.09LDD2071 pKa = 3.42YY2072 pKa = 11.03TLGANQEE2079 pKa = 4.18GLNLLGTLAVVGQGNEE2095 pKa = 4.18LGNMLNGNALDD2106 pKa = 3.82NTLMGLAGNDD2116 pKa = 3.44RR2117 pKa = 11.84LDD2119 pKa = 3.97GKK2121 pKa = 10.7QGADD2125 pKa = 3.39TLIGGLGNDD2134 pKa = 3.81TYY2136 pKa = 11.54YY2137 pKa = 11.13VDD2139 pKa = 3.45HH2140 pKa = 6.76TADD2143 pKa = 3.25VVTEE2147 pKa = 3.96QANEE2151 pKa = 4.25GVDD2154 pKa = 3.42TVYY2157 pKa = 11.4ASVSHH2162 pKa = 6.25TLADD2166 pKa = 3.56NVEE2169 pKa = 4.46TLRR2172 pKa = 11.84MTADD2176 pKa = 3.27GQTAIGNAANNVIYY2190 pKa = 10.42GSAGQDD2196 pKa = 3.32VLIDD2200 pKa = 3.81GGEE2203 pKa = 4.26GNDD2206 pKa = 3.89TIYY2209 pKa = 10.95TLGDD2213 pKa = 3.11KK2214 pKa = 10.95GGEE2217 pKa = 3.98QHH2219 pKa = 5.7VHH2221 pKa = 6.25GGLGSDD2227 pKa = 3.5TLYY2230 pKa = 11.4VSGTLANGDD2239 pKa = 3.61RR2240 pKa = 11.84GSYY2243 pKa = 10.51VYY2245 pKa = 11.14GDD2247 pKa = 4.41AGDD2250 pKa = 4.08DD2251 pKa = 3.79TLNATQGDD2259 pKa = 4.27VKK2261 pKa = 10.94SDD2263 pKa = 3.54LMGGAGNDD2271 pKa = 3.23TYY2273 pKa = 11.52VINNIHH2279 pKa = 6.04NRR2281 pKa = 11.84VIEE2284 pKa = 4.09NVNDD2288 pKa = 4.83GYY2290 pKa = 10.75DD2291 pKa = 3.43TVQTSVSFSLQAGEE2305 pKa = 4.18MFIPDD2310 pKa = 3.88SVDD2313 pKa = 3.4VPVTTGNVEE2322 pKa = 4.07RR2323 pKa = 11.84LLMTGTANIDD2333 pKa = 3.34GFGNEE2338 pKa = 4.27LVNYY2342 pKa = 10.33LLGNAGNNVLLGRR2355 pKa = 11.84DD2356 pKa = 3.63GADD2359 pKa = 3.04TLVGQGGNDD2368 pKa = 3.82LLDD2371 pKa = 4.16GGNGNDD2377 pKa = 3.25TLAPVTHH2384 pKa = 7.0ASSLGTPNVHH2394 pKa = 6.56TGQITLTGGNGSDD2407 pKa = 4.2VFWMTQGYY2415 pKa = 9.88VGDD2418 pKa = 4.45HH2419 pKa = 6.27SSGGNTLTLSDD2430 pKa = 4.6FVHH2433 pKa = 6.57GQDD2436 pKa = 3.31KK2437 pKa = 11.4VKK2439 pKa = 9.72FTLSLSATLPTTLTTLTPSGGATLASLLNQAAVAGSASAPKK2480 pKa = 8.85VTAFAFAGDD2489 pKa = 3.93TYY2491 pKa = 11.47LVLDD2495 pKa = 3.76QTSASSFTSDD2505 pKa = 3.92DD2506 pKa = 3.61LAIKK2510 pKa = 10.73LSGTPAFTFSDD2521 pKa = 3.73LAFVKK2526 pKa = 9.84MM2527 pKa = 4.41

Molecular weight:
259.32 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1U9JK21|A0A1U9JK21_9NEIS Lon protease OS=Aquaspirillum sp. LM1 OX=1938604 GN=lon PE=2 SV=1
MM1 pKa = 7.45KK2 pKa = 10.34KK3 pKa = 10.09SALHH7 pKa = 6.09AGKK10 pKa = 8.35TGKK13 pKa = 7.04TQRR16 pKa = 11.84SRR18 pKa = 11.84LARR21 pKa = 11.84PTAKK25 pKa = 9.28ATKK28 pKa = 8.76PLKK31 pKa = 10.38KK32 pKa = 9.81SVRR35 pKa = 11.84HH36 pKa = 5.21PQVKK40 pKa = 9.8SSKK43 pKa = 9.01PRR45 pKa = 11.84QGKK48 pKa = 10.33GNVAKK53 pKa = 9.66TKK55 pKa = 10.69ASTRR59 pKa = 11.84QHH61 pKa = 4.9TQRR64 pKa = 11.84QRR66 pKa = 11.84KK67 pKa = 7.63RR68 pKa = 11.84QQQ70 pKa = 2.66

Molecular weight:
7.88 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3268

0

3268

1103201

26

3224

337.6

36.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.418 ± 0.063

1.058 ± 0.017

5.467 ± 0.033

5.086 ± 0.039

3.323 ± 0.023

7.747 ± 0.047

2.578 ± 0.021

4.313 ± 0.031

3.144 ± 0.036

11.643 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.323 ± 0.021

2.847 ± 0.035

5.063 ± 0.044

4.813 ± 0.043

6.511 ± 0.051

5.628 ± 0.039

5.079 ± 0.054

7.071 ± 0.039

1.511 ± 0.021

2.376 ± 0.025

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski